LOCUS       sequence-391055-       15810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutated SMG1 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15810)
  AUTHORS   McCann JJ, Fleenor DE, Chen J, Lai CH, Bass TE, Kastan MB
  TITLE     Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced 
            Alternative Splicing Pathway.
  JOURNAL   Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1.
  PUBMED    36921295
REFERENCE   2  (bases 1 to 15810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1667/RADE-22-00219"; journalName: "Radiat Res"; date: 
            "2023-04-1- 1"; volume: "199"; issue: "4"; pages: "406-421"
COMMENT     Sequence Label: GFP SMG1 T3550A/S3556A
FEATURES             Location/Qualifiers
     source          1..15810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        26..329
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        330..533
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     484..504
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      572..581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(578..580,581..583,584..1294)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(578..580,581..583,584..1294)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(623..644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(884..903)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1231..1252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    6626..6637
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    12387..12439
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    12563..12684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12600..12619)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12654..12673
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(12691..13146)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12828..12849)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13040..13059
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        13173..13277
                     /gene="bla"
                     /label=AmpR promoter
     promoter        13279..13636
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        13279..13470
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(13302..13322)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      13487..13622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13671..14465
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             13671..14465
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13725..13744)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14335..14354
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(14653..14672)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14697..14744
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      15073..15661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15562..15581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
       61 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      121 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      181 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      241 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      301 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      361 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      421 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      481 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      541 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
      601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      661 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      781 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      841 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1021 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1081 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1261 cgggatcact ctcggcatgg acgagctgta caagtactca gatctcgaga tccatgtcgt
     1321 gtcggggaag gtaatgagcc gcagagcccc ggggtctcgg ctgagcagcg gcggcggcgg
     1381 cggcggcacc aagtatccgc ggagctggaa tgactggcaa cccagaactg atagtgcatc
     1441 agccgaccca gataatttaa aatattcttc atccagagat agaggtggtt cttcctctta
     1501 cggactgcaa ccttcaaatt cagctgtggt gtctcggcaa aggcacgatg ataccagagt
     1561 ccacgctgac atacagaatg acgaaaaggg tggctacagt gtcaatggag gatctgggga
     1621 aaatacttat ggtcggaagt cgttggggca agagctgagg gttaacaatg tgaccagccc
     1681 tgagttcacc agtgttcagc atggcagtcg tgctttagcc accaaagaca tgaggaaatc
     1741 acaggagaga tcgatgtctt attctgatga gtctcgactg tcgaatcttc ttcggaggat
     1801 cacccgggaa gacgacagag accgaagatt ggctactgta aagcagttga aagaatttat
     1861 tcagcaacca gaaaataagc tggtactagt taaacaattg gataatatct tggctgctgt
     1921 acatgacgtg cttaatgaaa gtagcaaatt gcttcaggag ttgagacagg agggagcttg
     1981 ctgtcttggc cttctttgtg cttctctgag ctatgaggct gagaagatct tcaagtggat
     2041 ttttagcaaa tttagctcat ctgcaaaaga tgaagttaaa ctcctctact tatgtgccac
     2101 ctacaaagca ctagagactg taggagaaaa gaaagccttt tcatctgtaa tgcagcttgt
     2161 aatgaccagc ctgcagtcta ttcttgaaaa tgtggataca ccagaattgc tttgtaaatg
     2221 tgttaagtgc attcttttgg tggctcgatg ttaccctcat attttcagca ctaattttag
     2281 ggatacagtt gatatattag ttggatggca tatagatcat actcagaaac cttcgctcac
     2341 gcagcaggta tctgggtggt tgcagagttt ggagccattt tgggtagctg atcttgcatt
     2401 ttctactact cttcttggtc agtttctgga agacatggaa gcatatgctg aggacctcag
     2461 ccatgtggcc tctggggaat cagtggatga agatgtccct cctccatcag tgtcattacc
     2521 aaagctggct gcacttctcc gggtatttag tactgtggtg aggagcattg gggaacgctt
     2581 cagcccaatt cggggtcctc caattactga ggcatatgta acagatgttc tgtacagagt
     2641 aatgagatgt gtgacggctg caaaccaggt gtttttttct gaggctgtgt tgacagctgc
     2701 taatgagtgt gttggtgttt tgctcggcag cttggatcct agcatgacta tacattgtga
     2761 catggtcatt acatatggat tagaccaact ggagaattgc cagacttgtg gtaccgatta
     2821 tatcatctca gtcttgaatt tactcacgct gattgttgaa cagataaata cgaaactgcc
     2881 atcatcattt gtagaaaaac tgtttatacc atcatctaaa ctactattct tgcgttatca
     2941 taaagaaaaa gaggttgttg ctgtagccca tgctgtttat caagcagtgc tcagcttgaa
     3001 gaatattcct gttttggaga ctgcctataa gttaatattg ggagaaatga cttgtgccct
     3061 aaacaacctc ctacacagtc tacaacttcc tgaggcctgt tctgaaataa aacatgaggc
     3121 ttttaagaat catgtgttca atgtagacaa tgcaaaattt gtagttatat ttgacctcag
     3181 tgccctgact acaattggaa atgccaaaaa ctcactaata gggatgtggg cgctatctcc
     3241 aactgtcttt gcacttctga gtaagaatct gatgattgtg cacagtgacc tggctgttca
     3301 cttccctgcc attcagtatg ctgtgctcta cacattgtat tctcattgta ccaggcatga
     3361 tcactttatc tctagtagcc tcagttcttc ctctccttct ttgtttgatg gagctgtgat
     3421 tagcactgta actacggcta caaagaaaca tttctcaatt atattaaatc ttctgggaat
     3481 attacttaag aaagataacc ttaaccagga cacgaggaaa ctgttaatga cttgggcttt
     3541 ggaagcagct gttttaatga agaagtctga aacatacgca cctttattct ctcttccgtc
     3601 tttccataaa ttttgcaaag gccttttagc caacactctc gttgaagatg tgaatatctg
     3661 tctgcaggca tgcagcagtc tacatgctct gtcctcttcc ttgccagatg atcttttaca
     3721 gagatgtgtc gatgtttgcc gtgttcaact agtgcacagt ggaactcgta ttcgacaagc
     3781 atttggaaaa ctgttgaaat caattccttt agatgttgtc ctaagcaata acaatcacac
     3841 agaaattcaa gaaatttctt tagcattaag aagtcacatg agtaaagcac caagtaatac
     3901 attccacccc caagatttct ctgatgttat tagttttatt ttgtatggga actctcatag
     3961 aacagggaag gacaattggt tggaaagact gttctatagc tgccagagac tggataagcg
     4021 tgaccagtca acaattccac gcaatctcct gaagacagat gctgtccttt ggcagtgggc
     4081 catatgggaa gctgcacaat tcactgttct ttctaagctg agaaccccac tgggcagagc
     4141 tcaagacacc ttccagacaa ttgaaggtat cattcgaagt ctcgcagctc acacattaaa
     4201 ccctgatcag gatgttagtc agtggacaac tgcagacaat gatgaaggcc atggtaacaa
     4261 ccaacttaga cttgttcttc ttctgcagta tctggaaaat ctggagaaat taatgtataa
     4321 tgcatacgag ggatgtgcta atgcattaac ttcacctccc aaggtcatta gaactttttt
     4381 ctataccaat cgccaaactt gtcaggactg gctaacgcgg attcgactct ccatcatgag
     4441 ggtaggattg ttggcaggcc agcctgcagt gacagtgaga catggctttg acttgcttac
     4501 agagatgaaa acaaccagcc tatctcaggg gaatgaattg gaagtaacca ttatgatggt
     4561 ggtagaagca ttatgtgaac ttcattgtcc tgaagctata cagggaattg ctgtctggtc
     4621 atcatctatt gttggaaaaa atcttctgtg gattaactca gtggctcaac aggctgaagg
     4681 gaggtttgaa aaggcctctg tggagtacca ggaacacctg tgtgccatga caggtgttga
     4741 ttgctgcatc tccagctttg acaaatcggt gctcacctta gccaatgctg ggcgtaacag
     4801 tgccagcccg aaacattctc tgaatggtga atccagaaaa actgtgctgt ccaaaccgac
     4861 tgactcttcc cctgaggtta taaattattt aggaaataaa gcatgtgagt gctacatctc
     4921 aattgccgat tgggctgctg tgcaggaatg gcagaacgct atccatgact tgaaaaagag
     4981 taccagtagc acttccctca acctgaaagc tgacttcaac tatataaaat cattaagcag
     5041 ctttgagtct ggaaaatttg ttgaatgtac cgagcagtta gaattgttac caggagaaaa
     5101 tatcaatcta cttgctggag gatcaaaaga aaaaatagac atgaaaaaac tgcttcctaa
     5161 catgttaagt ccggatccga gggaacttca gaaatccatt gaagttcaat tgttaagaag
     5221 ttctgtttgt ttggcaactg ctttaaaccc gatagaacaa gatcagaagt ggcagtctat
     5281 aactgaaaat gtggtaaagt acttgaagca aacatcccgc atcgctattg gacctctgag
     5341 actttctact ttaacagttt cacagtcttt gccagttcta agtaccttgc agctgtattg
     5401 ctcatctgct ttggagaaca cagtttctaa cagactttca acagaggact gtcttattcc
     5461 actcttcagt gaagctttac gttcatgtaa acagcatgac gtgaggccat ggatgcaggc
     5521 attaaggtat actatgtacc agaatcagtt gttggagaaa attaaagaac aaacagtccc
     5581 aattagaagc catctcatgg aattaggtct aacagcagca aaatttgcta gaaaacgagg
     5641 gaatgtgtcc cttgcaacaa gactgctggc acagtgcagt gaagttcagc tgggaaagac
     5701 caccactgca caggatttag tccaacattt taaaaaacta tcaacccaag gtcaagtgga
     5761 tgaaaaatgg gggcccgaac ttgatattga aaaaaccaaa ttgctttata cagcaggcca
     5821 gtcaacacat gcaatggaaa tgttgagttc ttgtgccata tctttctgca agtctgtgaa
     5881 agctgaatat gcagttgcta aatcaattct gacactggct aaatggatcc aggcagaatg
     5941 gaaagagatt tcaggacagc tgaaacaggt ttacagagct cagcaccaac agaacttcac
     6001 aggtctttct actttgtcta aaaacatact cactctaata gaactgccat ctgttaatac
     6061 gatggaagaa gagtatcctc ggatcgagag tgaatctaca gtgcatattg gagttggaga
     6121 acctgacttc attttgggac agttatatca cctgtcttca gtacaggcac ctgaagtagc
     6181 caaatcttgg gcagcgttgg ccagctgggc ttataggtgg ggcagaaagg tggttgacaa
     6241 tgccagtcag ggagaaggtg ttcgtctgct gcctagagaa aaatctgaag ttcagaatct
     6301 acttccagac actataactg aggaagagaa agagagaata tatggtattc ttggacaggc
     6361 tgtgtgtcgg ccggcgggga ttcaggatga agatataaca cttcagataa ctgagagtga
     6421 agacaacgaa gaagatgaca tggttgatgt tatctggcgt cagttgatat caagctgccc
     6481 atggctttca gaacttgatg aaagtgcaac tgaaggagtt attaaagtgt ggaggaaagt
     6541 tgtagataga atattcagcc tgtacaaact ctcttgcagt gcatacttta ctttccttaa
     6601 actcaacgct ggtcaaattc ctttagatga ggatgaccct aggctgcatt taagtcacag
     6661 agtggaacag agcactgatg acatgattgt gatggccaca ttgcgcctgc tgcggttgct
     6721 cgtgaagcat gctggtgagc ttcggcagta tctggagcac ggcttggaga caacacccac
     6781 tgcaccatgg aggggaatta ttccgcaact tttctcacgc ttaaaccacc ctgaagtgta
     6841 tgtgcgccaa agtatttgta accttctctg ccgtgtggct caagattccc cacatctcat
     6901 attgtatcct gcaatagtgg gtaccatatc gcttagtagt gaatcccagg cttcaggaaa
     6961 taaattttcc actgcaattc caactttact tggcaatatt caaggagaag aattgctggt
     7021 ttctgaatgt gagggaggaa gtcctcctgc atctcaggat agcaataagg atgaacctaa
     7081 aagtggatta aatgaagacc aagccatgat gcaggattgt tacagcaaaa ttgtagataa
     7141 gctgtcctct gcaaacccca ccatggtatt acaggttcag atgctcgtgg ctgaactgcg
     7201 cagggtcact gtgctctggg atgagctctg gctgggagtt ttgctgcaac aacacatgta
     7261 tgtcctgaga cgaattcagc agcttgaaga tgaggtgaag agagtccaga acaacaacac
     7321 cttacgcaaa gaagagaaaa ttgcaatcat gagggagaag cacacagctt tgatgaagcc
     7381 catcgtattt gctttggagc atgtgaggag tatcacagcg gctcctgcag aaacacctca
     7441 tgaaaaatgg tttcaggata actatggtga tgccattgaa aatgccctag aaaaactgaa
     7501 gactccattg aaccctgcaa agcctgggag cagctggatt ccatttaaag agataatgct
     7561 aagtttgcaa cagagagcac agaaacgtgc aagttacatc ttgcgtcttg aagaaatcag
     7621 tccatggttg gctgccatga ctaacactga aattgctctt cctggggaag tctcagccag
     7681 agacactgtc acaatccata gtgtgggcgg aaccatcaca atcttaccga ctaaaaccaa
     7741 gccaaagaaa cttctctttc ttggatcaga tgggaagagc tatccttatc ttttcaaagg
     7801 actggaggat ttacatctgg atgagagaat aatgcagttc ctatctattg tgaataccat
     7861 gtttgctaca attaatcgcc aagaaacacc ccggttccat gctcgacact attctgtaac
     7921 accactagga acaagatcag gactaatcca gtgggtagat ggagccacac ccttatttgg
     7981 tctttacaaa cgatggcaac aacgggaagc tgccttacaa gcacaaaagg cccaagattc
     8041 ctaccaaact cctcagaatc ctggaattgt accccgtcct agtgaacttt attacagtaa
     8101 aattggccct gctttgaaaa cagttgggct tagcctggat gtgtcccgtc gggattggcc
     8161 tcttcatgta atgaaggcag tattggaaga gttaatggag gccacacccc cgaatctcct
     8221 tgccaaagag ctctggtcat cttgcacaac acctgatgaa tggtggagag ttacgcagtc
     8281 ttatgcaaga tctactgcag tcatgtctat ggttggatac ataattggcc ttggagacag
     8341 acatctggat aatgttctta tagatatgac gactggagaa gttgttcaca tagattacaa
     8401 tgtttgcttt gaaaaaggta aaagccttag agttcctgag aaagtacctt ttcgaatgac
     8461 acaaaacatt gaaacagcac tgggtgtaac tggagtagaa ggtgtattta ggctttcatg
     8521 tgagcaggtt ttacacatta tgcggcgtgg cagagagacc ctgctgacgc tgctggaggc
     8581 ctttgtgtac gaccctctgg tggactggac agcaggaggc gaggctgggt ttgctggtgc
     8641 tgtctatggt ggaggtggcc agcaggccga gagcaagcag agcaagagag agatggagcg
     8701 agagatcacc cgcagcctgt tttcttctag agtagctgag attaaggtga actggtttaa
     8761 gaatagagat gagatgctgg ttgtgcttcc caagttggac ggtagcttag atgaatacct
     8821 aagcttgcaa gagcaactga cagatgtgga aaaactgcag ggcaaactac tggaggaaat
     8881 agagtttcta gaaggagctg aaggggtgga tcatccttct catactctgc aacacaggta
     8941 ttctgagcac acccaactac agactcagca aagagctgtt caggaagcaa tccaggtgaa
     9001 gctgaatgaa tttgaacaat ggataacaca ttatcaggct gcattcaata atttagaagc
     9061 aacacagctt gcaagcttgc ttcaagagat aagcacacaa atggaccttg gtcctccaag
     9121 ttacgtgcca gcaacagcct ttctgcagaa tgctggtcag gcccacttga ttagccagtg
     9181 cgagcagctg gagggggagg ttggtgctct cctgcagcag aggcgctccg tgctccgtgg
     9241 ctgtctggag caactgcatc actatgcaac cgtggccctg cagtatccga aggccatatt
     9301 tcagaaacat cgaattgaac agtggaagac ctggatggaa gagctcatct gtaacaccac
     9361 agtagagcgt tgtcaagagc tctataggaa atatgaaatg caatatgctc cccagccacc
     9421 cccaacagtg tgtcagttca tcactgccac tgaaatgacc ctgcagcgat acgcagcaga
     9481 catcaacagc agacttatta gacaagtgga acgcttgaaa caggaagctg tcactgtgcc
     9541 agtttgtgaa gatcagttga aagaaattga acgttgcatt aaagttttcc ttcatgagaa
     9601 tggagaagaa ggatctttga gtctagcaag tgttattatt tctgcccttt gtacccttac
     9661 aaggcgtaac ctgatgatgg aaggtgcagc gtcaagtgct ggagaacagc tggttgatct
     9721 gacttctcgg gatggagcct ggttcttgga ggaactctgc agtatgagcg gaaacgtcac
     9781 ctgcttggtt cagttactga agcagtgcca cctggtgcca caggacttag atatcccgaa
     9841 ccccatggaa gcgtctgaga cagttcactt agccaatgga gtgtatacct cacttcagga
     9901 attgaattcg aatttccggc aaatcatatt tccagaagca cttcgatgtt taatgaaagg
     9961 ggaatacacg ttagaaagta tgctgcatga actggacggt cttattgagc agaccaccga
    10021 tggcgttccc ctgcagactc tagtggaatc tcttcaggcc tacttaagaa acgcagctat
    10081 gggactggaa gaagaaacac atgctcatta catcgatgtt gccagactac tacatgctca
    10141 gtacggtgaa ttaatccaac cgagaaatgg ttcagttgat gaaacaccca aaatgtcagc
    10201 tggccagatg cttttggtag cattcgatgg catgtttgct caagttgaaa ctgctttcag
    10261 cttattagtt gaaaagttga acaagatgga aattcccata gcttggcgaa agattgacat
    10321 cataagggaa gccaggagta ctcaagttaa tttttttgat gatgataatc accggcaggt
    10381 gctagaagag attttctttc taaaaagact acagactatt aaggagttct tcaggctctg
    10441 tggtaccttt tctaaaacat tgtcaggatc aagttcactt gaagatcaga atactgtgaa
    10501 tgggcctgta cagattgtca atgtgaaaac cctttttaga aactcttgtt tcagtgaaga
    10561 ccaaatggcc aaacctatca aggcattcac agctgacttt gtgaggcagc tcttgatagg
    10621 gctacccaac caagccctcg gactcacact gtgcagtttt atcagtgctc tgggtgtaga
    10681 catcattgct caagtagagg caaaggactt tggtgccgaa agcaaagttt ctgttgatga
    10741 tctctgtaag aaagcggtgg aacataacat ccagataggg aagttctctc agctggttat
    10801 gaacagggca actgtgttag caagttctta cgacactgcc tggaagaagc atgacttggt
    10861 gcgaaggcta gaaaccagta tttcttcttg taagacaagc ctgcagcggg ttcagctgca
    10921 tattgccatg tttcagtggc aacatgaaga tctacttatc aatagaccac aagccatgtc
    10981 agtcacacct cccccacggt ctgctatcct aaccagcatg aaaaagaagc tgcataccct
    11041 gagccagatt gaaacttcta ttgcgacagt tcaggagaag ctagctgcac ttgaatcaag
    11101 tattgaacag cgactcaagt gggcaggtgg tgccaaccct gcattggccc ctgtactaca
    11161 agattttgaa gcaacgatag ctgaaagaag aaatcttgtc cttaaagaga gccaaagagc
    11221 aagtcaggtc acatttctct gcagcaatat cattcatttt gaaagtttac gaacaagaac
    11281 tgcagaagcc ttaaacctgg atgcggcgtt atttgaacta atcaagcgat gtcagcagat
    11341 gtgttcgttt gcatcacagt ttaacagttc agtgtctgag ttagagcttc gtttattaca
    11401 gagagtggac actggtcttg aacatcctat tggcagctct gaatggcttt tgtcagcaca
    11461 caaacagttg acccaggata tgtctactca gagggcaatt cagacagaga aagagcagca
    11521 gatagaaacg gtctgtgaaa caattcagaa tctggttgat aatataaaga ctgtgctcac
    11581 tggtcataac cgacagcttg gagatgtcaa acatctcttg aaagctatgg ctaaggatga
    11641 agaagctgct ctggcagatg gtgaagatgt tccctatgag aacagtgtta ggcagttttt
    11701 gggtgaatat aaatcatggc aagacaacat tcaaacagtt ctatttacat tagtccaggc
    11761 tatgggtcag gttcgaagtc aagaacacgt tgaaatgctc caggaaatca ctcccacctt
    11821 gaaagaactg aaaacacaaa gtcagagtat ctataataat ttagtgagtt ttgcatcacc
    11881 cttagtcacc gatgcaacaa atgaatgttc gagtccaacg tcatctgcta cttatcagcc
    11941 atccttcgct gcagcagtcc ggagtaacac tggccagaag gctcagcctg atgtcatggc
    12001 acagaatgct agaaagctga tccagaaaaa tcttgctaca tcagctgata ctccaccaag
    12061 caccgttcca ggaactggca agagtgttgc ttgtagtcct aaaaaggcag tcagagaccc
    12121 taaaactggg aaagcggtgc aagagagaaa ctcctatgca gtgagtgtgt ggaagagagt
    12181 gaaagccaag ttagagggcc gagatgttga tccgaatagg aggatgtcag ttgctgaaca
    12241 ggttgactat gtcattaagg aagcaactaa tctagataac ttggctcagc tgtatgaagg
    12301 ttggacagcc tgggtgtgaa tggcaagaca gtagatgagt ctggttaagc gaggtcagac
    12361 atccaccaga atcaactcga cctcgactcg agctcaagct tcgaattctg cagtcgacgg
    12421 taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
    12481 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    12541 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    12601 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12661 gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat
    12721 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
    12781 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
    12841 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
    12901 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
    12961 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
    13021 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
    13081 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
    13141 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    13201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    13261 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
    13321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    13381 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    13441 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    13501 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    13561 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    13621 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
    13681 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
    13741 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
    13801 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
    13861 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
    13921 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
    13981 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
    14041 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
    14101 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    14161 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
    14221 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    14281 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    14341 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    14401 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    14461 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
    14521 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    14581 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
    14641 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
    14701 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
    14761 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
    14821 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
    14881 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
    14941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    15001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    15061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    15121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    15181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    15241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    15301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    15361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    15421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    15481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    15541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    15601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    15661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    15721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt
    15781 attaatagta atcaattacg gggtcattag
//
