LOCUS       sequence-391056-       15810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses WT SMG1 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15810)
  AUTHORS   McCann JJ, Fleenor DE, Chen J, Lai CH, Bass TE, Kastan MB
  TITLE     Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced 
            Alternative Splicing Pathway.
  JOURNAL   Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1.
  PUBMED    36921295
REFERENCE   2  (bases 1 to 15810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1667/RADE-22-00219"; journalName: "Radiat Res"; date: 
            "2023-04-1- 1"; volume: "199"; issue: "4"; pages: "406-421"
COMMENT     Sequence Label: GFP SMG1 WT
FEATURES             Location/Qualifiers
     source          1..15810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        143..446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        447..650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     601..621
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      689..698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(695..697,698..700,701..1411)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(695..697,698..700,701..1411)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(740..761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1001..1020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1348..1369
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    6743..6754
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    12504..12556
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    12680..12801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12717..12736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12771..12790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(12808..13263)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12945..12966)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13157..13176
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        13290..13394
                     /gene="bla"
                     /label=AmpR promoter
     promoter        13396..13753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        13396..13587
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(13419..13439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      13604..13739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13788..14582
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             13788..14582
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13842..13861)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14452..14471
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(14770..14789)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14814..14861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      15190..15778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15679..15698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
       61 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
      121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
      661 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
      721 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      781 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      841 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      901 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      961 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1021 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1081 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1141 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1201 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1261 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1321 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1381 gatcactctc ggcatggacg agctgtacaa gtactcagat ctcgagatcc atgtcgtgtc
     1441 ggggaaggta atgagccgca gagccccggg gtctcggctg agcagcggcg gcggcggcgg
     1501 cggcaccaag tatccgcgga gctggaatga ctggcaaccc agaactgata gtgcatcagc
     1561 cgacccagat aatttaaaat attcttcatc cagagataga ggtggttctt cctcttacgg
     1621 actgcaacct tcaaattcag ctgtggtgtc tcggcaaagg cacgatgata ccagagtcca
     1681 cgctgacata cagaatgacg aaaagggtgg ctacagtgtc aatggaggat ctggggaaaa
     1741 tacttatggt cggaagtcgt tggggcaaga gctgagggtt aacaatgtga ccagccctga
     1801 gttcaccagt gttcagcatg gcagtcgtgc tttagccacc aaagacatga ggaaatcaca
     1861 ggagagatcg atgtcttatt ctgatgagtc tcgactgtcg aatcttcttc ggaggatcac
     1921 ccgggaagac gacagagacc gaagattggc tactgtaaag cagttgaaag aatttattca
     1981 gcaaccagaa aataagctgg tactagttaa acaattggat aatatcttgg ctgctgtaca
     2041 tgacgtgctt aatgaaagta gcaaattgct tcaggagttg agacaggagg gagcttgctg
     2101 tcttggcctt ctttgtgctt ctctgagcta tgaggctgag aagatcttca agtggatttt
     2161 tagcaaattt agctcatctg caaaagatga agttaaactc ctctacttat gtgccaccta
     2221 caaagcacta gagactgtag gagaaaagaa agccttttca tctgtaatgc agcttgtaat
     2281 gaccagcctg cagtctattc ttgaaaatgt ggatacacca gaattgcttt gtaaatgtgt
     2341 taagtgcatt cttttggtgg ctcgatgtta ccctcatatt ttcagcacta attttaggga
     2401 tacagttgat atattagttg gatggcatat agatcatact cagaaacctt cgctcacgca
     2461 gcaggtatct gggtggttgc agagtttgga gccattttgg gtagctgatc ttgcattttc
     2521 tactactctt cttggtcagt ttctggaaga catggaagca tatgctgagg acctcagcca
     2581 tgtggcctct ggggaatcag tggatgaaga tgtccctcct ccatcagtgt cattaccaaa
     2641 gctggctgca cttctccggg tatttagtac tgtggtgagg agcattgggg aacgcttcag
     2701 cccaattcgg ggtcctccaa ttactgaggc atatgtaaca gatgttctgt acagagtaat
     2761 gagatgtgtg acggctgcaa accaggtgtt tttttctgag gctgtgttga cagctgctaa
     2821 tgagtgtgtt ggtgttttgc tcggcagctt ggatcctagc atgactatac attgtgacat
     2881 ggtcattaca tatggattag accaactgga gaattgccag acttgtggta ccgattatat
     2941 catctcagtc ttgaatttac tcacgctgat tgttgaacag ataaatacga aactgccatc
     3001 atcatttgta gaaaaactgt ttataccatc atctaaacta ctattcttgc gttatcataa
     3061 agaaaaagag gttgttgctg tagcccatgc tgtttatcaa gcagtgctca gcttgaagaa
     3121 tattcctgtt ttggagactg cctataagtt aatattggga gaaatgactt gtgccctaaa
     3181 caacctccta cacagtctac aacttcctga ggcctgttct gaaataaaac atgaggcttt
     3241 taagaatcat gtgttcaatg tagacaatgc aaaatttgta gttatatttg acctcagtgc
     3301 cctgactaca attggaaatg ccaaaaactc actaataggg atgtgggcgc tatctccaac
     3361 tgtctttgca cttctgagta agaatctgat gattgtgcac agtgacctgg ctgttcactt
     3421 ccctgccatt cagtatgctg tgctctacac attgtattct cattgtacca ggcatgatca
     3481 ctttatctct agtagcctca gttcttcctc tccttctttg tttgatggag ctgtgattag
     3541 cactgtaact acggctacaa agaaacattt ctcaattata ttaaatcttc tgggaatatt
     3601 acttaagaaa gataacctta accaggacac gaggaaactg ttaatgactt gggctttgga
     3661 agcagctgtt ttaatgaaga agtctgaaac atacgcacct ttattctctc ttccgtcttt
     3721 ccataaattt tgcaaaggcc ttttagccaa cactctcgtt gaagatgtga atatctgtct
     3781 gcaggcatgc agcagtctac atgctctgtc ctcttccttg ccagatgatc ttttacagag
     3841 atgtgtcgat gtttgccgtg ttcaactagt gcacagtgga actcgtattc gacaagcatt
     3901 tggaaaactg ttgaaatcaa ttcctttaga tgttgtccta agcaataaca atcacacaga
     3961 aattcaagaa atttctttag cattaagaag tcacatgagt aaagcaccaa gtaatacatt
     4021 ccacccccaa gatttctctg atgttattag ttttattttg tatgggaact ctcatagaac
     4081 agggaaggac aattggttgg aaagactgtt ctatagctgc cagagactgg ataagcgtga
     4141 ccagtcaaca attccacgca atctcctgaa gacagatgct gtcctttggc agtgggccat
     4201 atgggaagct gcacaattca ctgttctttc taagctgaga accccactgg gcagagctca
     4261 agacaccttc cagacaattg aaggtatcat tcgaagtctc gcagctcaca cattaaaccc
     4321 tgatcaggat gttagtcagt ggacaactgc agacaatgat gaaggccatg gtaacaacca
     4381 acttagactt gttcttcttc tgcagtatct ggaaaatctg gagaaattaa tgtataatgc
     4441 atacgaggga tgtgctaatg cattaacttc acctcccaag gtcattagaa cttttttcta
     4501 taccaatcgc caaacttgtc aggactggct aacgcggatt cgactctcca tcatgagggt
     4561 aggattgttg gcaggccagc ctgcagtgac agtgagacat ggctttgact tgcttacaga
     4621 gatgaaaaca accagcctat ctcaggggaa tgaattggaa gtaaccatta tgatggtggt
     4681 agaagcatta tgtgaacttc attgtcctga agctatacag ggaattgctg tctggtcatc
     4741 atctattgtt ggaaaaaatc ttctgtggat taactcagtg gctcaacagg ctgaagggag
     4801 gtttgaaaag gcctctgtgg agtaccagga acacctgtgt gccatgacag gtgttgattg
     4861 ctgcatctcc agctttgaca aatcggtgct caccttagcc aatgctgggc gtaacagtgc
     4921 cagcccgaaa cattctctga atggtgaatc cagaaaaact gtgctgtcca aaccgactga
     4981 ctcttcccct gaggttataa attatttagg aaataaagca tgtgagtgct acatctcaat
     5041 tgccgattgg gctgctgtgc aggaatggca gaacgctatc catgacttga aaaagagtac
     5101 cagtagcact tccctcaacc tgaaagctga cttcaactat ataaaatcat taagcagctt
     5161 tgagtctgga aaatttgttg aatgtaccga gcagttagaa ttgttaccag gagaaaatat
     5221 caatctactt gctggaggat caaaagaaaa aatagacatg aaaaaactgc ttcctaacat
     5281 gttaagtccg gatccgaggg aacttcagaa atccattgaa gttcaattgt taagaagttc
     5341 tgtttgtttg gcaactgctt taaacccgat agaacaagat cagaagtggc agtctataac
     5401 tgaaaatgtg gtaaagtact tgaagcaaac atcccgcatc gctattggac ctctgagact
     5461 ttctacttta acagtttcac agtctttgcc agttctaagt accttgcagc tgtattgctc
     5521 atctgctttg gagaacacag tttctaacag actttcaaca gaggactgtc ttattccact
     5581 cttcagtgaa gctttacgtt catgtaaaca gcatgacgtg aggccatgga tgcaggcatt
     5641 aaggtatact atgtaccaga atcagttgtt ggagaaaatt aaagaacaaa cagtcccaat
     5701 tagaagccat ctcatggaat taggtctaac agcagcaaaa tttgctagaa aacgagggaa
     5761 tgtgtccctt gcaacaagac tgctggcaca gtgcagtgaa gttcagctgg gaaagaccac
     5821 cactgcacag gatttagtcc aacattttaa aaaactatca acccaaggtc aagtggatga
     5881 aaaatggggg cccgaacttg atattgaaaa aaccaaattg ctttatacag caggccagtc
     5941 aacacatgca atggaaatgt tgagttcttg tgccatatct ttctgcaagt ctgtgaaagc
     6001 tgaatatgca gttgctaaat caattctgac actggctaaa tggatccagg cagaatggaa
     6061 agagatttca ggacagctga aacaggttta cagagctcag caccaacaga acttcacagg
     6121 tctttctact ttgtctaaaa acatactcac tctaatagaa ctgccatctg ttaatacgat
     6181 ggaagaagag tatcctcgga tcgagagtga atctacagtg catattggag ttggagaacc
     6241 tgacttcatt ttgggacagt tatatcacct gtcttcagta caggcacctg aagtagccaa
     6301 atcttgggca gcgttggcca gctgggctta taggtggggc agaaaggtgg ttgacaatgc
     6361 cagtcaggga gaaggtgttc gtctgctgcc tagagaaaaa tctgaagttc agaatctact
     6421 tccagacact ataactgagg aagagaaaga gagaatatat ggtattcttg gacaggctgt
     6481 gtgtcggccg gcggggattc aggatgaaga tataacactt cagataactg agagtgaaga
     6541 caacgaagaa gatgacatgg ttgatgttat ctggcgtcag ttgatatcaa gctgcccatg
     6601 gctttcagaa cttgatgaaa gtgcaactga aggagttatt aaagtgtgga ggaaagttgt
     6661 agatagaata ttcagcctgt acaaactctc ttgcagtgca tactttactt tccttaaact
     6721 caacgctggt caaattcctt tagatgagga tgaccctagg ctgcatttaa gtcacagagt
     6781 ggaacagagc actgatgaca tgattgtgat ggccacattg cgcctgctgc ggttgctcgt
     6841 gaagcatgct ggtgagcttc ggcagtatct ggagcacggc ttggagacaa cacccactgc
     6901 accatggagg ggaattattc cgcaactttt ctcacgctta aaccaccctg aagtgtatgt
     6961 gcgccaaagt atttgtaacc ttctctgccg tgtggctcaa gattccccac atctcatatt
     7021 gtatcctgca atagtgggta ccatatcgct tagtagtgaa tcccaggctt caggaaataa
     7081 attttccact gcaattccaa ctttacttgg caatattcaa ggagaagaat tgctggtttc
     7141 tgaatgtgag ggaggaagtc ctcctgcatc tcaggatagc aataaggatg aacctaaaag
     7201 tggattaaat gaagaccaag ccatgatgca ggattgttac agcaaaattg tagataagct
     7261 gtcctctgca aaccccacca tggtattaca ggttcagatg ctcgtggctg aactgcgcag
     7321 ggtcactgtg ctctgggatg agctctggct gggagttttg ctgcaacaac acatgtatgt
     7381 cctgagacga attcagcagc ttgaagatga ggtgaagaga gtccagaaca acaacacctt
     7441 acgcaaagaa gagaaaattg caatcatgag ggagaagcac acagctttga tgaagcccat
     7501 cgtatttgct ttggagcatg tgaggagtat cacagcggct cctgcagaaa cacctcatga
     7561 aaaatggttt caggataact atggtgatgc cattgaaaat gccctagaaa aactgaagac
     7621 tccattgaac cctgcaaagc ctgggagcag ctggattcca tttaaagaga taatgctaag
     7681 tttgcaacag agagcacaga aacgtgcaag ttacatcttg cgtcttgaag aaatcagtcc
     7741 atggttggct gccatgacta acactgaaat tgctcttcct ggggaagtct cagccagaga
     7801 cactgtcaca atccatagtg tgggcggaac catcacaatc ttaccgacta aaaccaagcc
     7861 aaagaaactt ctctttcttg gatcagatgg gaagagctat ccttatcttt tcaaaggact
     7921 ggaggattta catctggatg agagaataat gcagttccta tctattgtga ataccatgtt
     7981 tgctacaatt aatcgccaag aaacaccccg gttccatgct cgacactatt ctgtaacacc
     8041 actaggaaca agatcaggac taatccagtg ggtagatgga gccacaccct tatttggtct
     8101 ttacaaacga tggcaacaac gggaagctgc cttacaagca caaaaggccc aagattccta
     8161 ccaaactcct cagaatcctg gaattgtacc ccgtcctagt gaactttatt acagtaaaat
     8221 tggccctgct ttgaaaacag ttgggcttag cctggatgtg tcccgtcggg attggcctct
     8281 tcatgtaatg aaggcagtat tggaagagtt aatggaggcc acacccccga atctccttgc
     8341 caaagagctc tggtcatctt gcacaacacc tgatgaatgg tggagagtta cgcagtctta
     8401 tgcaagatct actgcagtca tgtctatggt tggatacata attggccttg gagacagaca
     8461 tctggataat gttcttatag atatgacgac tggagaagtt gttcacatag attacaatgt
     8521 ttgctttgaa aaaggtaaaa gccttagagt tcctgagaaa gtaccttttc gaatgacaca
     8581 aaacattgaa acagcactgg gtgtaactgg agtagaaggt gtatttaggc tttcatgtga
     8641 gcaggtttta cacattatgc ggcgtggcag agagaccctg ctgacgctgc tggaggcctt
     8701 tgtgtacgac cctctggtgg actggacagc aggaggcgag gctgggtttg ctggtgctgt
     8761 ctatggtgga ggtggccagc aggccgagag caagcagagc aagagagaga tggagcgaga
     8821 gatcacccgc agcctgtttt cttctagagt agctgagatt aaggtgaact ggtttaagaa
     8881 tagagatgag atgctggttg tgcttcccaa gttggacggt agcttagatg aatacctaag
     8941 cttgcaagag caactgacag atgtggaaaa actgcagggc aaactactgg aggaaataga
     9001 gtttctagaa ggagctgaag gggtggatca tccttctcat actctgcaac acaggtattc
     9061 tgagcacacc caactacaga ctcagcaaag agctgttcag gaagcaatcc aggtgaagct
     9121 gaatgaattt gaacaatgga taacacatta tcaggctgca ttcaataatt tagaagcaac
     9181 acagcttgca agcttgcttc aagagataag cacacaaatg gaccttggtc ctccaagtta
     9241 cgtgccagca acagcctttc tgcagaatgc tggtcaggcc cacttgatta gccagtgcga
     9301 gcagctggag ggggaggttg gtgctctcct gcagcagagg cgctccgtgc tccgtggctg
     9361 tctggagcaa ctgcatcact atgcaaccgt ggccctgcag tatccgaagg ccatatttca
     9421 gaaacatcga attgaacagt ggaagacctg gatggaagag ctcatctgta acaccacagt
     9481 agagcgttgt caagagctct ataggaaata tgaaatgcaa tatgctcccc agccaccccc
     9541 aacagtgtgt cagttcatca ctgccactga aatgaccctg cagcgatacg cagcagacat
     9601 caacagcaga cttattagac aagtggaacg cttgaaacag gaagctgtca ctgtgccagt
     9661 ttgtgaagat cagttgaaag aaattgaacg ttgcattaaa gttttccttc atgagaatgg
     9721 agaagaagga tctttgagtc tagcaagtgt tattatttct gccctttgta cccttacaag
     9781 gcgtaacctg atgatggaag gtgcagcgtc aagtgctgga gaacagctgg ttgatctgac
     9841 ttctcgggat ggagcctggt tcttggagga actctgcagt atgagcggaa acgtcacctg
     9901 cttggttcag ttactgaagc agtgccacct ggtgccacag gacttagata tcccgaaccc
     9961 catggaagcg tctgagacag ttcacttagc caatggagtg tatacctcac ttcaggaatt
    10021 gaattcgaat ttccggcaaa tcatatttcc agaagcactt cgatgtttaa tgaaagggga
    10081 atacacgtta gaaagtatgc tgcatgaact ggacggtctt attgagcaga ccaccgatgg
    10141 cgttcccctg cagactctag tggaatctct tcaggcctac ttaagaaacg cagctatggg
    10201 actggaagaa gaaacacatg ctcattacat cgatgttgcc agactactac atgctcagta
    10261 cggtgaatta atccaaccga gaaatggttc agttgatgaa acacccaaaa tgtcagctgg
    10321 ccagatgctt ttggtagcat tcgatggcat gtttgctcaa gttgaaactg ctttcagctt
    10381 attagttgaa aagttgaaca agatggaaat tcccatagct tggcgaaaga ttgacatcat
    10441 aagggaagcc aggagtactc aagttaattt ttttgatgat gataatcacc ggcaggtgct
    10501 agaagagatt ttctttctaa aaagactaca gactattaag gagttcttca ggctctgtgg
    10561 taccttttct aaaacattgt caggatcaag ttcacttgaa gatcagaata ctgtgaatgg
    10621 gcctgtacag attgtcaatg tgaaaaccct ttttagaaac tcttgtttca gtgaagacca
    10681 aatggccaaa cctatcaagg cattcacagc tgactttgtg aggcagctct tgatagggct
    10741 acccaaccaa gccctcggac tcacactgtg cagttttatc agtgctctgg gtgtagacat
    10801 cattgctcaa gtagaggcaa aggactttgg tgccgaaagc aaagtttctg ttgatgatct
    10861 ctgtaagaaa gcggtggaac ataacatcca gatagggaag ttctctcagc tggttatgaa
    10921 cagggcaact gtgttagcaa gttcttacga cactgcctgg aagaagcatg acttggtgcg
    10981 aaggctagaa accagtattt cttcttgtaa gacaagcctg cagcgggttc agctgcatat
    11041 tgccatgttt cagtggcaac atgaagatct acttatcaat agaccacaag ccatgtcagt
    11101 cacacctccc ccacggtctg ctatcctaac cagcatgaaa aagaagctgc ataccctgag
    11161 ccagattgaa acttctattg cgacagttca ggagaagcta gctgcacttg aatcaagtat
    11221 tgaacagcga ctcaagtggg caggtggtgc caaccctgca ttggcccctg tactacaaga
    11281 ttttgaagca acgatagctg aaagaagaaa tcttgtcctt aaagagagcc aaagagcaag
    11341 tcaggtcaca tttctctgca gcaatatcat tcattttgaa agtttacgaa caagaactgc
    11401 agaagcctta aacctggatg cggcgttatt tgaactaatc aagcgatgtc agcagatgtg
    11461 ttcgtttgca tcacagttta acagttcagt gtctgagtta gagcttcgtt tattacagag
    11521 agtggacact ggtcttgaac atcctattgg cagctctgaa tggcttttgt cagcacacaa
    11581 acagttgacc caggatatgt ctactcagag ggcaattcag acagagaaag agcagcagat
    11641 agaaacggtc tgtgaaacaa ttcagaatct ggttgataat ataaagactg tgctcactgg
    11701 tcataaccga cagcttggag atgtcaaaca tctcttgaaa gctatggcta aggatgaaga
    11761 agctgctctg gcagatggtg aagatgttcc ctatgagaac agtgttaggc agtttttggg
    11821 tgaatataaa tcatggcaag acaacattca aacagttcta tttacattag tccaggctat
    11881 gggtcaggtt cgaagtcaag aacacgttga aatgctccag gaaatcactc ccaccttgaa
    11941 agaactgaaa acacaaagtc agagtatcta taataattta gtgagttttg catcaccctt
    12001 agtcaccgat gcaacaaatg aatgttcgag tccaacgtca tctgctactt atcagccatc
    12061 cttcgctgca gcagtccgga gtaacactgg ccagaagact cagcctgatg tcatgtcaca
    12121 gaatgctaga aagctgatcc agaaaaatct tgctacatca gctgatactc caccaagcac
    12181 cgttccagga actggcaaga gtgttgcttg tagtcctaaa aaggcagtca gagaccctaa
    12241 aactgggaaa gcggtgcaag agagaaactc ctatgcagtg agtgtgtgga agagagtgaa
    12301 agccaagtta gagggccgag atgttgatcc gaataggagg atgtcagttg ctgaacaggt
    12361 tgactatgtc attaaggaag caactaatct agataacttg gctcagctgt atgaaggttg
    12421 gacagcctgg gtgtgaatgg caagacagta gatgagtctg gttaagcgag gtcagacatc
    12481 caccagaatc aactcgacct cgactcgagc tcaagcttcg aattctgcag tcgacggtac
    12541 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
    12601 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    12661 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    12721 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    12781 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
    12841 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
    12901 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
    12961 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
    13021 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
    13081 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
    13141 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
    13201 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
    13261 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    13321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    13381 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
    13441 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    13501 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    13561 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    13621 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    13681 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    13741 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
    13801 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    13861 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    13921 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
    13981 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    14041 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    14101 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    14161 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    14221 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    14281 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
    14341 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    14401 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    14461 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    14521 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    14581 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    14641 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    14701 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
    14761 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
    14821 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
    14881 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
    14941 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
    15001 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
    15061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    15121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    15181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    15241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    15301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    15361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    15421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    15481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    15541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    15601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    15661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    15721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    15781 cgccagcaac gcggcctttt tacggttcct
//
