LOCUS       sequence-391060-        3580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-SlRPS5A1-6
FEATURES             Location/Qualifiers
     source          1..3580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1390..1398
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(1460..1478)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1546..1650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1872..2663
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2834..3422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3323..3342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3576..3580,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagccaacac caagtgatat actaaccatg
      121 taaataatcc aaatttatcc aaaaatatca ttatctaaag tgtgtaaaca aaaatcaaat
      181 atgaaatact tacatacctt tccgtatctg accgtttaac tctaaaattg aagctagttt
      241 taattttgta tttcatcatt acagccacaa gagttgcttt atccttgtac aattgtttcg
      301 tctacacatt taatatattt gtatctgata tataattttc tttctccatc tcgggtatgt
      361 aaaaagaatc accaatgttt gattcaacaa cgtttagagc attagcatct ctttgtccac
      421 cttcaataca cataattgca ccactgcttg aataaaaaat ttgtaactcc tctgtacttt
      481 tatcatttct atcgatgcaa agcggataca tactgaatcc aacctcttgc ttctttattt
      541 ctataaacag cttcacacca atatcattcc taattatcat cggagaagaa ttgccctcta
      601 caatatatcg gataataata ttttttttca attcatcaat atttaattca gtagcaattg
      661 ctgagattag attcatatat gaaatatttt ccccaaccac tatcccatca cttttgtatt
      721 gattgtattt aacctcactc tcccatattc ctgaatgcct tagcaatatc gtaatattca
      781 ttacgaaacc ggatacaaaa ttaccttcaa ggaaattttg aatatcgttt gtgccaaaaa
      841 aatggaagat cactaatcaa aatcaaaata tcatttttgc caaaaataaa aagaaagatc
      901 actaatgaca aaatttaagg gaataaaact gattttaaaa cacacaagtt aatgccttta
      961 tttaagggaa taataaccgt ggcatattaa aagtaggaat taattcaaat ttcagttttt
     1021 aatcccgcaa attactcaac atacaaataa aaatccccca aattactcaa caaacggata
     1081 aaatgaaata ctatataatg tatccgacaa ttttattatg tatcataaac ttattaaaaa
     1141 taaaagattt tttgtaaatt gaaaaagaat agggattgga ggtaatttat attttacact
     1201 atgtgattta cataattttt acatttttta ttgaaaaact ttagaagttg cgcaagtagc
     1261 caaaaaaaca aactggagca agttgagtta ggaaaagtgg gccaaatagg ctttttagcc
     1321 caatcttaga atgaaggcta ttttggacag tataagtggg tttagggttt tccttcttct
     1381 ccaatcgaca cctccttaga cctatcagaa aaacaggaaa aatgtgagac cacgaagtgg
     1441 ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1501 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1561 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1621 atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg
     1681 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     1741 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     1801 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     1861 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     1921 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     1981 cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa
     2041 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2101 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2161 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2221 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     2281 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     2341 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2401 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     2461 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     2521 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     2581 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga
     2641 tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg
     2701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3541 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
//
