LOCUS       sequence-391061-        3491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-SlUbi-6
FEATURES             Location/Qualifiers
     source          1..3491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1371..1389)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1457..1561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1783..2574
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2745..3333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3234..3253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3487..3491,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagacataaa ttcttttttt gtaatgctca
      121 aataaatttg agtaaaaaag aatgaaattg agtgattttt ttttaatcat aagaaaataa
      181 ataattaatt tcaatataat aaaacagtaa tataatttca taaatggaat tcaatactta
      241 cctcttagat ataaaaaata aatataaaaa taaagtgttt ctaataaacc cgcaatttaa
      301 ataaaatatt taatattttc aatcaaattt aaataattat attaaaatat cgtagaaaaa
      361 gagcaatata taatacaaga aagaagattt aagtacaatt atcaactatt attatactct
      421 aattttgtta tatttaattt cttacggtta aggtcatgtt cacgataaac tcaaaatacg
      481 ctgtatgagg acatatttta aattttaacc aataataaaa ctaagttatt tttagtatat
      541 ttttttgttt aacgtgactt aatttttctt ttctagagga gcgtgtaagt gtcaacctca
      601 ttctcctaat tttcccaacc acataaaaaa aaaataaagg tagcttttgc gtgttgattt
      661 ggtacactac acgtcattat tacacgtgtt ttcgtatgat tggttaatcc atgaggcggt
      721 ttcctctaga gtcggccata ccatctataa aataaagctt tctgcagctc attttttcat
      781 cttctatctg atttctatta taatttctct gaattgcctt caaatttctc tttcaaggtt
      841 agaatttttc tctatttttt ggtttttgtt tgtttagatt ctgagtttag ttaatcaggt
      901 gctgttaaag ccctaaattt tgagtttttt tcggttgttt tgatggaaaa tacctaacaa
      961 ttgagttttt tcatgttgtt ttgtcggaga atgcctacaa ttggagttcc tttcgttgtt
     1021 ttgatgagaa agcccctaat ttgagtgttt ttccgtcgat ttgattttaa aggtttatat
     1081 tcgagttttt ttcgtcggtt taatgagaag gcctaaaata ggagtttttc tggttgattt
     1141 gactaaaaaa gccatggaat tttgtgtttt tgatgtcgct ttggttctca aggcctaaga
     1201 tctgagtttc tccggttgtt ttgatgaaaa agccctaaaa ttggagtttt tatcttgtgt
     1261 tttaggttgt tttaatcctt ataatttgag ttttttcgtt gttctgattg ttgtttttat
     1321 gaattttgca gaatgtgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat
     1381 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1441 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1501 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1561 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     1621 ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca
     1681 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1741 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     1801 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1861 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc
     1921 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     1981 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2041 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2101 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2161 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2221 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2281 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2341 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2401 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2461 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2521 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
     2581 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2641 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2701 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2761 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2821 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2881 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2941 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3001 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3061 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3121 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3181 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3241 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3301 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3361 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3421 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3481 gagcgcagcg a
//
