LOCUS       sequence-391062-        3871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-StUbi-6
FEATURES             Location/Qualifiers
     source          1..3871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1751..1769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1837..1941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2163..2954
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3125..3713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3614..3633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3867..3871,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gaggatgaca agattttggt catcaattac
      121 atatacccaa attgaatagt aagcaactta atgtttttca taatgataat gacagacaca
      181 aaaaaaaccc atttattatt cacattgatt gatttttata tgcaatatag taataataat
      241 aatatttctt ataaagcaag aggtcaattt ttttttatta taccaacgtc actaaattat
      301 atttgataat gtaaaacaat tcaattttac ttaaatatca tgaaataaac tatttttata
      361 accaaattac taaatttttc caataaaaaa aagtcattaa gaacacataa aataaatttg
      421 agtaaaaaga gtgaagtcga ctgacttttt ttttttttat cataagaaaa taaattatta
      481 actttaacct aataaaacac taatataatt tcatggaatc taatacttac ctcttagaaa
      541 taagaaaaag tgtttctaat agaccctcaa tttacattaa atattttcaa tcaaatttaa
      601 ataacaaata tcaatatgag gtcaataaca atatcaaaat aatatgaaaa aagagcaata
      661 cataatataa gaaagaagat ttaagtgcga ttatcaaggt agtattatat cctaatttgc
      721 taatatttaa actcttatat ttaaggtcat gttcatgata aacttgaaat gcgctatatt
      781 agagcatata ttaaaataaa aaaataccta aaataaaatt aagttatttt tagtatatat
      841 ttttttacat gacctacatt tttctgggtt tttctaaagg agcgtgtaag tgtcgacctc
      901 attctcctaa ttttccccac cacataaaaa ttaaaaagga aaggtagctt ttgcgtgttg
      961 ttttggtaca ctacacctca ttattacacg tgtcctcata taattggtta accctatgag
     1021 gcggtttcgt ctagagtcgg ccatgccatc tataaaatga agctttctgc acctcatttt
     1081 tttcatcttc tatctgattt ctattataat ttctctcaat tgccttcaaa tttctcttta
     1141 aggttagaaa tcttctctat ttttggtttt tgtctgttta gattctcgaa ttagctaatc
     1201 aggtgctgtt atagccctta attttgagtt ttttttcggt tgttttgatg gaaaaggcct
     1261 aaaatttgag tttttttacg ttggtttgat ggaaaaggcc tacaattgga gttttccccg
     1321 ttgttttgat gaaaaagccc ctagtttgag attttttttc tgtcgattcg attctaaagg
     1381 tttaaaatta gagtttttac atttgtttga tgaaaaaggc cttaaatttg agtttttccg
     1441 gttgatttga tgaaaaagcc ctagaatttg tgttttttcg tcggtttgat tctgaaggcc
     1501 taaaatttga gtttctccgg ctgttttgat gaaaaagccc taaatttgag tttctccggc
     1561 tgttttgatg aaaaagccct aaatttgagt tttttccccg tgttttagat tgtttggttt
     1621 taattctcga atcagctaat cagggagtgt gaaaagccct aaaatttgag tttttttcgt
     1681 tgttctgatt gttgttttta tgaatttgca gaatgtgaga ccacgaagtg gctcttcagt
     1741 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1801 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1921 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     1981 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg
     2041 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2101 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2161 tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2221 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2281 gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2341 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2401 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2461 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2521 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2581 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2641 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2701 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2761 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2821 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2881 cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg
     2941 tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3001 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3061 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3841 ctcgccgcag ccgaacgacc gagcgcagcg a
//
