LOCUS       sequence-391063-        3346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-ZmAXIG1-6
FEATURES             Location/Qualifiers
     source          1..3346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1226..1244)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1312..1416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1638..2429
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2600..3188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3089..3108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3342..3346,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gaggcgaccc atcgctgctt tgtctacatc
      121 atgttcttca tcatcctccc caggcgacgc gtgctgctgt tcttattcag actaccgttc
      181 gagtgactgc atggcgtaca tctttctgca tcgactttgt acggctacat cgaacatata
      241 cacgagatgt ctcgtgtgaa tagagtcact aatgccttaa gcatcggtta ctccgtaggg
      301 tacattctgt tcttcttatt tgtgcatatt tttattgttg tttactgatt atacgagtag
      361 ttatacatac atgcacatac atatcatcac atatatcaca atatttttct aaattaaatt
      421 aaaactaaaa atgactaaat ttctaacacc aacgacattg taatgttttc tccaacaact
      481 ttacctattc tacattgttc tatttcgaat ttcactctat aaacaacata gtctacaatg
      541 gaaaacagtg ctttgtacga ctatatacgc gatgtgtggc tacaacataa gacaatatag
      601 tcgtttgaag attgaaccta tatatcggta cggttaatcc gtctatgtac gtgggcatga
      661 cgaacacccg tgataacgaa ggattaacgt gcacaatcat aaatccaaag taggagcggt
      721 gcatgatgag aatcgctctc agtactcgac ataatgaacc ttacgaggta caacaggcag
      781 gcaggcaggg accaggggcc gcctttattt caggctcgct ggccccacgg gcgtgctgcg
      841 tgcacgaagg gcactacccc aacctctcac cgaaaaccgc gctggatcgg caaatcaaac
      901 gaggtggtgc cccgtgccca ctctccacgt ccacggcacc atccctctgc agccgctcac
      961 cagccatgcc gtgtcgcgga acggcacaac cacccccaac ccactcacga aaccccgtcc
     1021 cggccgtgcc cgtgtcggtc cgcgctcggc aacgaggcgg cccgcgctgc tgagtcccct
     1081 ggacacccga caccctgtcg gccctttgtt tattcatccc gaaatctcat ctgcccccac
     1141 ggccgactgc gctgcgccgc ccggatatat atacccatcg ttatcgaatg tgagaccacg
     1201 aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1261 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1321 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1381 tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga
     1441 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1501 tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1561 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1621 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1681 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1741 gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga
     1801 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1861 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1921 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     1981 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2041 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2101 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2161 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2221 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2281 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2341 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtaaaag
     2401 gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca tatatacttt
     2461 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2521 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2581 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2641 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2701 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2761 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2821 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2941 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3061 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcga
//
