LOCUS       sequence-391064-        4342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-ZmEf1a-6
FEATURES             Location/Qualifiers
     source          1..4342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2222..2240)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2308..2412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2634..3425
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3596..4184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4085..4104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4338..4342,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagatcttga tttattcctt atgtcatgaa
      121 ttccttttta tttacaccca cactttctaa ctttcgtaca atgattcgta tatttaaaac
      181 aaggctaact tatgttctca tgctttacga accctggcat atgtcgcaaa aaatccttac
      241 ctaaagaact aactctgcag cctcttcaag ctctagattt caattcttat ggcctaagaa
      301 gtgttttttt atttcataat ataagctttc acaacaatct aattcttgga gtcagattct
      361 ataaataccc aacatttact aacctacatt tttttttcaa ttggtgccat gaccatcaca
      421 taatgttgat gagctattcc ctccaagttg ctgattgcat gtggattacc atgcatggct
      481 atttgctatt tggttgtccc gacctttctt aggatctatt ggatgtaata catattatca
      541 ttatttagct aatatttgct aactactacc tagcagagtg gatagtaggt tgtggtgttt
      601 atatccataa ctaatgggtt aattaataat tggagagtaa tgttcctgtt agcccccatt
      661 gtttgggtag atagtaagat aatggggatt tagaaacaat ctaaagttta ttacaatctg
      721 ttatctagac ctatttatat ccatagagct aaagtttagc agattgctac atcctcatta
      781 ttagtttgga gccaaacatg tcctgattgt ataatttaaa tgtttatagg agtaaccaat
      841 ttgttttagc tttgctattt tacgagtgtc aagatgctta actatttcat acttgctttg
      901 taggaatgtt cgatgccgac aacatcttgc tctaccagaa ggtaccagct ccaaatcaag
      961 aaccaaaatt taggaaaaat acgaactgca accttatccg tcatctttag taattcatgt
     1021 gtttatctta tttgcaccaa gaactatgct ggaggctata aaactatgaa tgttatttgt
     1081 gacatgtaaa taatactagc ttgtacatgc atctaaatgc atataagaaa cacaatgcac
     1141 cttttgtttg cattccatgt aaattataaa ttaaatccaa acattgaaaa attacaaaac
     1201 acacacacac aacactatgc gcgcatgggc gcgcgcaaga cactagtaca taataataac
     1261 gatattgttc gcttcggatt aaattaaacc aagcgagtat gcgtgcatgt gtacgtcgga
     1321 tgctttgttt gttcatttgt ggcccgtgaa gtaatatttg ggcccaatag cccattcatc
     1381 catgtcctaa ccctagggcg cgttccttat aaaacctatc tccattctgt tctcactctc
     1441 agacaccaga cgcagtcggc tgccgcagac tggtagtagc cggcgcgccg tcaccgtatc
     1501 ctcagatcag aggcgagcag aaaccacacc actctactga cgcccgacga ggtacacttc
     1561 cgcgcgcgcg cttctcttcc ttcttttttc gctcgacgag gtactctgct tcacgcgcgg
     1621 gcgcgcttct cttccttctc ttttcgctct gccttgagcc tgttcgtttg atgcccagat
     1681 ctactaggtt tgtcgatcgt cgttatgtgt gatgagctga gccgattcat gcactgtatt
     1741 tttaattaag tgtttttttc ctccgccgct acctttattg tctatgtatg aacttgcagc
     1801 ttacaactgg ttattgagcc ataaattgta actgagtttc cgagatctat aatattacgt
     1861 ggctgaggcg cttcatctct gttttgtttg tatgacagta ggagtactaa tccaagttta
     1921 gcttccgctt cttcatctac ctttagtatg tatttcgtat ataaatacga ttgcagtttg
     1981 caactgattc tcggccatag atgattaccg ttgcgttgtt catcgggtct agtttcggat
     2041 ctctgatgtt gtgtgggtgg ggtacttcga cttgggtttt cctgcccttt gtttgattac
     2101 tggtttattg acatatattt atctattgac tttttatctg ctatgtttga tgtaagcagc
     2161 agtgtaacat tttccttcag ccaatgtgag accacgaagt ggctcttcag tggacgaaag
     2221 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2281 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2401 aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     2461 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta
     2521 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2581 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg
     2641 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2701 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2761 agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2821 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2881 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2941 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     3001 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3061 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3121 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3181 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3241 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3301 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3361 cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca
     3421 aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3481 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3541 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3601 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3661 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3721 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3781 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3841 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3901 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3961 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4021 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4081 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4141 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4201 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4261 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4321 gccgaacgac cgagcgcagc ga
//
