LOCUS       sequence-391065-        3309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-ZmPLTP-6
FEATURES             Location/Qualifiers
     source          1..3309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1189..1207)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1275..1379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1601..2392
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2563..3151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3052..3071
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3305..3309,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagctccctg cggctgttac gcggtccccc
      121 atcaatcttc tgttcttgcg gttgtagcct gtgtaacagt gctagagtat gtatgataaa
      181 taggttttaa gtctgcttac atgacatttt ttattgtgga agagacatat aaaaattaga
      241 gagagtggtt ctcatgcaac ggcggacggc ccggtgctaa aagagcttca agacaaaata
      301 atgaaacagg aagagagtag atttatctaa gagccaactt tattatatga atgtgtttat
      361 tgttggcttt agatgatatg gtaaggagtt agagctaata atagataggc tctattatta
      421 ttattattaa ttaaactcgc tctaaggagg aaagtgggag gaagggacga ggacgtagac
      481 tactggaagc atcgtgcatg gatgatggat gtggtgtctc ttaatgtagg tggccggagg
      541 atgtacgtgt taattgcgcg ataagcactc agatccaacc gcaaactacc tccacactga
      601 cacactgata gagagaaaga cagacctccg acgactgccg ccgcagatga gccacgtacg
      661 tatacgacgt ctgccggccg gctcaggctg ccgccatcac cctgctcgaa agtcgcgtta
      721 ggcggcgcca gctacatagg agtatctagt ctagccagtt agtatactac tactgcgctg
      781 atgatgaatt aactctgcat agatactgta cttgcctccc tccaacaccc aaccacctcc
      841 tgctcggctc ttaataactt ggacacggat cgatgccatc caaggaagaa cacgacgacg
      901 acgacggaac atccaccatg caagcttgca tccatacgcc gatacgcgtg catccatcca
      961 tccaccatta tttccatttt ccaccgatca cacgtacaca ggcctattta aggagcgaca
     1021 tcccactgca actctcctca ccactcatca ccagctagct ctagcaaagc acttgccatc
     1081 taccgaccgc cgcattccaa acagcccgac gagctagcag agcggcaggc acctccctcc
     1141 tcaaggaaca atgtgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
     1201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1381 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     1441 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
     1501 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     1561 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     1621 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     1681 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     1741 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     1801 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     1861 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     1921 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     1981 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     2041 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     2101 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     2161 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     2221 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     2281 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     2341 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
     2401 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2461 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2581 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2641 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2701 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2761 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2821 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2881 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2941 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3001 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3061 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3121 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3181 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3241 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3301 gcgcagcga
//
