LOCUS       sequence-391066-        3341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-BeYDV-Rep-RepA-9
FEATURES             Location/Qualifiers
     source          1..3341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1221..1239)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1307..1411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1633..2424
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2595..3183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3084..3103
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3337..3341,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgccttctg ctagtaagaa cttcagactc
      121 caatctaaat atgttttcct tacctatccc aagtgctcat ctcaaagaga tgatttattc
      181 cagtttctct gggagaaact cacacctttt cttattttct tccttggtgt tgcttctgag
      241 cttcatcaag atggcactac ccactatcat gctcttctcc agcttgataa aaaaccttgt
      301 attagggatc cttctttttt cgattttgaa ggaaatcacc ctaatatcca gccagctaga
      361 aactctaaac aagtccttga ttacatatca aaggacggag atattaaaac cagaggagat
      421 ttccgagatc ataaggtttc tcctcgcaaa tctgacgcac gatggagaac tattatccag
      481 actgcaacgt ctaaggagga atatcttgac atgatcaagg aagaattccc tcatgaatgg
      541 gcaacaaagc ttcaatggct ggaatattca gccaacaaat tattccctcc acaacctgaa
      601 ccgtatgtgt cgcccttcac agaatcagat cttcgctgcc acgaagatct acactcctgg
      661 agggaaaccc atctatacca tgtaagcata gacgcttata cttacataca tcctgtctca
      721 taccaacaag ctcaatctga ccttgaatgg atggccgatt taacaaggac aatggaagga
      781 atggaatccg acaccccagc ctctacatct gcggaccaac tcgtaccgga aagaccacct
      841 gggctagaag tctcggacga cacaactatt ggaacggtac catcgatttc accaactacg
      901 atgaacacgc cacctataat atcatcgacg acatcccctt caagttcgtc ccattgtgga
      961 agcaattaat aggttgccag tctgatttca ctgtcaaccc taaatatgga aaaaagaaga
     1021 aaataaaagg tgggatccct tctataattc tttgcaatcc tgacgaggac tggatgttat
     1081 caatgacaag tcaacagaag gattacttta aagataattg cgtcacccac tacatgtgtg
     1141 acggggagac tttttttgct cgggaatcgt cgagtcactg agctttgaga ccacgaagtg
     1201 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1261 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1381 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
     1441 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1501 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1561 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1621 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1681 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1741 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     1801 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1861 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1921 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1981 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     2041 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2101 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     2161 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     2221 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     2281 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     2341 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     2401 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     2461 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2521 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2581 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2641 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2701 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2761 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2821 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2881 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2941 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3001 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3061 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3121 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3181 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3241 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3301 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg a
//
