LOCUS       sequence-391067-        3392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-WDV-Rep-RepA-9
FEATURES             Location/Qualifiers
     source          1..3392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1272..1290)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1358..1462
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1684..2475
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2646..3234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3135..3154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3388..3392,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggcctctt catctgcacc caggttccgt
      121 gtctattcca agtacctctt tctaacatat cctcaatgta cccttgagcc acagtacgcc
      181 ttggattcac ttcgcactct cttgaacaaa tatgagcccc tctacatcgc tgctgttaga
      241 gagctgcacg aagatggatc accacatctg cacgttctcg tgcagaacaa gcttcgtgct
      301 tccatcacca atcccaatgc cttaaacctc cgtatggata catctccatt ctccatattc
      361 catccaaata tacaagctgc caaagactgc aaccaagttc gtgattacat cacgaaggag
      421 gttgactccg atgtaaacac agctgagtgg ggaacattcg tggctgtttc aactccaggt
      481 cgtaaagacc gtgatgcgga tatgaaacag atcattgaat ctagttcctc tcgcgaggaa
      541 ttcctcagca tggtttgcaa tcgttttccg tttgaatggt ctatccgcct caaagacttc
      601 gagtacacgg cacgccatct atttcctgac ccagttgcca cttacacacc tgagtttcca
      661 accgaatcac tcatttgcca tgagacaatt gaaagctgga aaaatgaaca tctctactcc
      721 gtaagcctcg aatcctatat cctttgtact tccactcctg cggatcaagc gcaatctgac
      781 ttagagtgga tggacgatta ttccaggagt caccggggag gcataagtcc atctacatct
      841 gcgggccaac cagaacagga aagacttcct gggcaaggtc tttagggaca cacaattatt
      901 ataacagtct agttgatttc acaacatatg acgtcaacgc caagtataat atcatcgacg
      961 acattccatt caagttcaca cccaactgga agtgcttcgt cggggctcag cgtgacttca
     1021 cggtcaatcc aaaatatggt aagcgaaagg tcatacgggg tggaatacct tgcatcattt
     1081 tagttaatcc agacgaagat tggctcaagg atatgactcc cgaacagtcg gattacatgt
     1141 actctaacac tgttgttcac tacatgtatg aaggcgagac attcatcaac tactcgttcg
     1201 cctccggcga agatgtcact gcttcgcagt gagctttgag accacgaagt ggctcttcag
     1261 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1321 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1381 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1441 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     1501 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg
     1561 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     1621 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     1681 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1741 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1801 ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1861 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     1921 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     1981 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     2041 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     2101 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     2161 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     2221 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     2281 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     2341 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     2401 gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag
     2461 gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2521 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2581 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2641 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2701 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2761 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2821 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2881 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2941 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3001 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3061 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3121 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3181 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3241 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3301 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3361 gctcgccgca gccgaacgac cgagcgcagc ga
//
