LOCUS       sequence-391068-        3022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-BeYDV-LS-L-11
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(902..920)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        988..1092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1314..2105
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2276..2864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2765..2784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3018..3022,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcaaatac catcacatcg tatatgcttt
      121 tatagtgtga acacctttaa ccctagtggg cgggaaattt tctactttaa atctggaccg
      181 ctcgtgctaa agcactcgcg ataagggggg gccacgccgg taatattaaa ttcggcgtgg
      241 gccccccctt gtcgcaaaga cttcgtcttt aagtaaattc cacgtcattt tccactatct
      301 attaaaatga ccaaaatacc cctgcctcca tgcctccacg ccggttataa gatagagttt
      361 gaggcaaccc ctcggagtca caacaacacg tgcctctcct catacgagtt tatctaaagt
      421 gattattttt tttggggtgt ttgtttttgg attgtctttt tttgtttatt tcgtgtgtta
      481 tgtaacatat gtaatttcta tctacttgca caatgaaata tattcataaa ataatcatta
      541 ccgtgagacg aacaacaacg tctctagtgc aaataccatc acatcgtata tgcttttata
      601 gtgtgaacac ctttaaccct agtgggcggg aaattttcta ctttaaatct ggaccgctcg
      661 tgctaaagca ctcgcgataa gggggggcca cgccggtaat attaaattcg gcgtgggccc
      721 ccccttgtcg caaagacttc gtctttaagt aaattccacg tcattttcca ctatctatta
      781 aaatgaccaa aatacccctg cctccatgcc tccacgccgg ttataagata gagtttgagg
      841 caacccctcg gagtcacaac aacgcttgag accacgaagt ggctcttcag tggacgaaag
      901 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      961 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1081 aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     1141 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta
     1201 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1261 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg
     1321 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     1381 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     1441 agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     1501 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     1561 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     1621 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     1681 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     1741 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     1801 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     1861 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1921 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1981 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2041 cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca
     2101 aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2161 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2221 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2281 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2341 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2401 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2461 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2521 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2581 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2641 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2701 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2761 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2821 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2881 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2941 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3001 gccgaacgac cgagcgcagc ga
//
