LOCUS       sequence-391070-        4412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-WDV-LRS-L-11
FEATURES             Location/Qualifiers
     source          1..4412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2292..2310)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2378..2482
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2704..3495
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3666..4254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4155..4174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4408..4412,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcgagatg ggctaccacg cacttcctta
      121 taagctaagg catggcacag atttccccgt caaaagtgtg ataaatcaac atcttccacg
      181 aaagtgatca gaaaagatgt ggtccaaaag cctccgcgca ctcacgaaaa gccgagtgcg
      241 cgtcgggggc caccacgcgg ggtaatatta taacccgcgt ggaggccccc cgaccgcaca
      301 ccgaacaggg cccacacgat tcgctacgct accgtgggaa cactagggcc ctgttctccc
      361 gccaaaacct gccctatata aagcattgga cacattgcat ttgcagtgtg cagaattcac
      421 acctccacgc agggtagagg atagagtttt ctggcacacc ccgttttttc gctaaggacc
      481 tgccggactt ctgttcacta ccatggcctc ttcatctgca cccaggttcc gtgtctattc
      541 caagtacctc tttctaacat atcctcaatg tacccttgag ccacagtacg ccttggattc
      601 acttcgcact ctcttgaaca aatatgagcc cctctacatc gctgctgtta gagagctgca
      661 cgaagatgga tcaccacatc tgcacgttct cgtgcagaac aagcttcgtg cttccatcac
      721 caatcccaat gccttaaacc tccgtatgga tacatctcca ttctccatat tccatccaaa
      781 tatacaagct gccaaagact gcaaccaagt tcgtgattac atcacgaagg aggttgactc
      841 cgatgtaaac acagctgagt ggggaacatt cgtggctgtt tcaactccag gtcgtaaaga
      901 ccgtgatgcg gatatgaaac agatcattga atctagttcc tctcgcgagg aattcctcag
      961 catggtttgc aatcgttttc cgtttgaatg gtctatccgc ctcaaagact tcgagtacac
     1021 ggcacgccat ctatttcctg acccagttgc cacttacaca cctgagtttc caaccgaatc
     1081 actcatttgc catgagacaa ttgaaagctg gaaaaatgaa catctctact ccgtaagcct
     1141 cgaatcctat atcctttgta cttccactcc tgcggatcaa gcgcaatctg acttagagtg
     1201 gatggacgat tattccagga gtcaccgggg aggcataagt ccatctacat ctgcgggcca
     1261 accagaacag gaaagacttc ctgggcaagg tctttaggga cacacaatta ttataacagt
     1321 ctagttgatt tcacaacata tgacgtcaac gccaagtata atatcatcga cgacattcca
     1381 ttcaagttca cacccaactg gaagtgcttc gtcggggctc agcgtgactt cacggtcaat
     1441 ccaaaatatg gtaagcgaaa ggtcatacgg ggtggaatac cttgcatcat tttagttaat
     1501 ccagacgaag attggctcaa ggatatgact cccgaacagt cggattacat gtactctaac
     1561 actgttgttc actacatgta tgaaggcgag acattcatca actactcgtt cgcctccggc
     1621 gaagatgtca ctgcttcgca gtgactgtcc tagcgcggac ggcgtacagt ttctataaat
     1681 gtaatatctc agggtgttgt atttcgtttt ccgatgtcat tgtgtaaggg ttgttgtttg
     1741 ttatatttgg tttgtctttg ctcgtagccg agcctctgta atcgaatgac atgagataaa
     1801 taaaatatta ttttaccgtg agacgaacaa caacgtctct agtgcgagat gggctaccac
     1861 gcacttcctt ataagctaag gcatggcaca gatttccccg tcaaaagtgt gataaatcaa
     1921 catcttccac gaaagtgatc agaaaagatg tggtccaaaa gcctccgcgc actcacgaaa
     1981 agccgagtgc gcgtcggggg ccaccacgcg gggtaatatt ataacccgcg tggaggcccc
     2041 ccgaccgcac accgaacagg gcccacacga ttcgctacgc taccgtggga acactagggc
     2101 cctgttctcc cgccaaaacc tgccctatat aaagcattgg acacattgca tttgcagtgt
     2161 gcagaattca cacctccacg cagggtagag gatagagttt tctggcacac cccgtttttt
     2221 cgctaaggac ctgccggact tctgttcact accgcttgag accacgaagt ggctcttcag
     2281 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2341 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2401 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2461 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     2521 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg
     2581 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     2641 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     2701 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     2761 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     2821 ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat
     2881 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     2941 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     3001 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     3061 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     3121 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     3181 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     3241 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     3301 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     3361 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     3421 gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag
     3481 gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3541 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4081 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4141 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4201 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4261 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4321 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4381 gctcgccgca gccgaacgac cgagcgcagc ga
//
