LOCUS       sequence-391071-        3281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-Cre-9
FEATURES             Location/Qualifiers
     source          1..3281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1161..1179)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1247..1351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1573..2364
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2535..3123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3024..3043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3277..3281,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgagcaact tgttgacggt ccatcaaaat
      121 cttccggcgt tgcccgtcga tgccacatcc gatgaagtca gaaagaacct catggatatg
      181 tttcgggatc ggcaagcatt tagcgaacac acttggaaga tgctcctgtc cgtctgtagg
      241 agctgggctg catggtgcaa actcaacaat agaaagtggt ttcccgccga acccgaggat
      301 gtgcgcgact acctcttgta cctgcaagcg agaggattgg cggtcaagac aatacagcag
      361 catctgggac aactcaatat gcttcatcgc cggtctggct tgcctagacc aagcgactct
      421 aatgcagttt cgttggtgat gcggcgcatt cgcaaagaga acgttgatgc tggggaaaga
      481 gccaaacagg ccttggcgtt tgaacgcact gacttcgacc aagtcaggag cctgatggag
      541 aacagcgacc gctgccaaga catcaggaac ctggcattcc tcgggatagc gtataacacg
      601 cttctccgca tagcggaaat tgctcggatt cgggtcaagg atatatcaag aacagacggc
      661 gggcggatgt tgattcacat cggacgcact aaaacgttgg tgtcaactgc cggggtggag
      721 aaggctctct ctttgggtgt cactaaactt gtggagcggt ggatctcggt cagcggcgtg
      781 gctgatgacc cgaataacta tcttttttgc cgggtcagaa aaaatggtgt ggcggcccct
      841 tcggcgacct cacaactctc gactcgggct ctggaaggca tatttgaagc gacgcatcgg
      901 ctgatttatg gtgccaaaga tgatagcggg caaaggtacc tcgcgtggtc tgggcactct
      961 gcgagagtgg gggcagcacg cgatatggcg cgcgctggtg tctcaatccc cgaaattatg
     1021 caagccgggg gctggaccaa tgttaatatc gtgatgaatt acattaggaa cctcgatagc
     1081 gagactgggg ccatggtgcg gcttcttgag gatggagatt agctttgaga ccacgaagtg
     1141 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1201 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1261 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1321 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
     1381 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1441 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1501 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1561 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1621 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1681 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     1741 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1801 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1861 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1921 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1981 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2041 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     2101 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     2161 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     2221 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     2281 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     2341 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     2401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2641 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3181 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3241 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg a
//
