LOCUS       sequence-391072-        3470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-Cre-StLS1-9
FEATURES             Location/Qualifiers
     source          1..3470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          335..523
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     primer_bind     complement(1350..1368)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1436..1540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1762..2553
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2724..3312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3213..3232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3466..3470,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgagcaact tgttgacggt ccatcaaaat
      121 cttccggcgt tgcccgtcga tgccacatcc gatgaagtca gaaagaacct catggatatg
      181 tttcgggatc ggcaagcatt tagcgaacac acttggaaga tgctcctgtc cgtctgtagg
      241 agctgggctg catggtgcaa actcaacaat agaaagtggt ttcccgccga acccgaggat
      301 gtgcgcgact acctcttgta cctgcaagcg cgaggtaagt ttctgcttct acctttgata
      361 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa
      421 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat
      481 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggtctggc ggtcaagaca
      541 atacagcagc atctgggaca actcaatatg cttcatcgcc ggtctggctt gcctagacca
      601 agcgactcta atgcagtttc gttggtgatg cggcgcattc gcaaagagaa cgttgatgct
      661 ggggaaagag ccaaacaggc cttggcgttt gaacgcactg acttcgacca agtcaggagc
      721 ctgatggaga acagcgaccg ctgccaagac atcaggaacc tggcattcct cgggatagcg
      781 tataacacgc ttctccgcat agcggaaatt gctcggattc gggtcaagga tatatcaaga
      841 acagacggcg ggcggatgtt gattcacatc ggacgcacta aaacgttggt gtcaactgcc
      901 ggggtggaga aggctctctc tttgggtgtc actaaacttg tggagcggtg gatctcggtc
      961 agcggcgtgg ctgatgaccc gaataactat cttttttgcc gggtcagaaa aaatggtgtg
     1021 gcggcccctt cggcgacctc acaactctcg actcgggctc tggaaggcat atttgaagcg
     1081 acgcatcggc tgatttatgg tgccaaagat gatagcgggc aaaggtacct cgcgtggtct
     1141 gggcactctg cgagagtggg ggcagcacgc gatatggcgc gcgctggtgt ctcaatcccc
     1201 gaaattatgc aagccggggg ctggaccaat gttaatatcg tgatgaatta cattaggaac
     1261 ctcgatagcg agactggggc catggtgcgg cttcttgagg atggagatta gctttgagac
     1321 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1381 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1441 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1501 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1561 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1621 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1681 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1741 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1801 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1861 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     1921 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     1981 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2041 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2101 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2161 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2221 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2281 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2341 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2401 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2461 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     2521 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     2581 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2641 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2701 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2761 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2821 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2881 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2941 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3001 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3061 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3361 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3421 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
//
