LOCUS       sequence-391073-        4310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-Cre-GR-9
FEATURES             Location/Qualifiers
     source          1..4310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          335..523
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     primer_bind     complement(2190..2208)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2276..2380
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2602..3393
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3564..4152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4053..4072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4306..4310,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgagcaact tgttgacggt ccatcaaaat
      121 cttccggcgt tgcccgtcga tgccacatcc gatgaagtca gaaagaacct catggatatg
      181 tttcgggatc ggcaagcatt tagcgaacac acttggaaga tgctcctgtc cgtctgtagg
      241 agctgggctg catggtgcaa actcaacaat agaaagtggt ttcccgccga acccgaggat
      301 gtgcgcgact acctcttgta cctgcaagcg cgaggtaagt ttctgcttct acctttgata
      361 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa
      421 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat
      481 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggtctggc ggtcaagaca
      541 atacagcagc atctgggaca actcaatatg cttcatcgcc ggtctggctt gcctagacca
      601 agcgactcta atgcagtttc gttggtgatg cggcgcattc gcaaagagaa cgttgatgct
      661 ggggaaagag ccaaacaggc cttggcgttt gaacgcactg acttcgacca agtcaggagc
      721 ctgatggaga acagcgaccg ctgccaagac atcaggaacc tggcattcct cgggatagcg
      781 tataacacgc ttctccgcat agcggaaatt gctcggattc gggtcaagga tatatcaaga
      841 acagacggcg ggcggatgtt gattcacatc ggacgcacta aaacgttggt gtcaactgcc
      901 ggggtggaga aggctctctc tttgggtgtc actaaacttg tggagcggtg gatctcggtc
      961 agcggcgtgg ctgatgaccc gaataactat cttttttgcc gggtcagaaa aaatggtgtg
     1021 gcggcccctt cggcgacctc acaactctcg actcgggctc tggaaggcat atttgaagcg
     1081 acgcatcggc tgatttatgg tgccaaagat gatagcgggc aaaggtacct cgcgtggtct
     1141 gggcactctg cgagagtggg ggcagcacgc gatatggcgc gcgctggtgt ctcaatcccc
     1201 gaaattatgc aagccggggg ctggaccaat gttaatatcg tgatgaatta cattaggaac
     1261 ctcgatagcg agactggggc catggtgcgg cttcttgagg atggagatgg atccggtatt
     1321 caacaagcta ctgcaggggt ttctcaagac acttcagaga atccaaataa gaccatagtt
     1381 cctgcagctc tgccccaact cactccgacg ttggtgagtt tgcttgaagt tatcgaacct
     1441 gaagtgctgt acgcgggtta cgattccagc gttccagatt ctgcttggcg cataatgacc
     1501 accttgaaca tgctgggagg aaggcaagtt atagccgccg tgaagtgggc taaggcgatc
     1561 cttggcttgc gcaatcttca cctggacgat caaatgacct tgctgcagta tagctggatg
     1621 ttccttatgg cgttcgctct cggttggaga agctatcggc agtcatccgg caacctgctt
     1681 tgcttcgccc cggacttgat aatcaatgag caaaggatgt ctttgccgtg catgtatgac
     1741 cagtgtaagc acatgctgtt cgtgtcatct gagcttcaaa ggttgcaagt cagttacgag
     1801 gaatatctct gcatgaaaac cctcctcctg ctttcatctg tgccaaagga gggactgaag
     1861 tcgcaagaac tcttcgacga gatccggatg acgtacatca aggagcttgg gaaagctata
     1921 gttaagagag agggaaactc ctcacagaac tggcaacggt tctatcaact caccaagctg
     1981 cttgattcaa tgcatgaggt ggttgaaaat ttgctcacct attgctttca gacgttcctt
     2041 gacaaaacca tgagtataga attccccgaa atgctggctg agatcataac gaaccagatc
     2101 ccaaagtatt ctaacggaaa tataaaaaaa cttctgtttc atcagaaata gctttgagac
     2161 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2221 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2341 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     2401 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     2461 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     2521 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     2581 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     2641 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2701 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     2761 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2821 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2881 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2941 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     3001 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     3061 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     3121 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     3181 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     3241 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     3301 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     3361 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     3421 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3481 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3541 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3601 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3661 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3721 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3781 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3841 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3901 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3961 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4021 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4081 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4141 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4201 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4261 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
//
