LOCUS       sequence-391074-        3548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-FLPe-9
FEATURES             Location/Qualifiers
     source          1..3548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             97..117
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1428..1446)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1514..1618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1840..2631
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2802..3390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3291..3310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3544..3548,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggctccaa aaaagaaacg aaaagtaatg
      121 agccagtttg acatcctctg taagactcct cctaaggtgc ttgtgcgtca atttgtcgaa
      181 cgtttcgagc gtccttctgg tgaaaagata gctagttgcg ccgccgagct tacctactta
      241 tgctggatga ttactcataa cggtactgca ataaagaggg ctacctttat gagttacaat
      301 actataatat caaacagctt gagttttgac atcgtaaata agagtttaca attcaaatac
      361 aaaactcaaa aagctacaat actcgaggca agtctcaaaa agcttattcc agcttgggaa
      421 tttacaataa tcccctataa cggccagaag catcaatctg acattactga catcgttagt
      481 agcctccaac tgcaatttga gagtagtgaa gaggctgaca aagggaactc acactcaaag
      541 aagatgctga aagctttatt atctgagggc gagagcattt gggaaatcac cgagaaaata
      601 ctcaatagtt ttgaatatac atctagattt accaagacaa aaacactgta ccaattccta
      661 ttcttagcca catttataaa ttgcgggaga ttctcagata tcaaaaacgt ggatcccaaa
      721 agctttaagt tagttcagaa caagtatctc ggggtaatta tccaatgttt agttactgaa
      781 accaagacaa gcgtgagtcg tcacatctac tttttttcag cacgagggag gatcgatcca
      841 ttggtttacc ttgacgaatt tctaagaaat tcagaaccag tattgaaacg tgtcaatcgt
      901 acagggaatt catcttcaaa taagcaggag taccaactat taaaagacaa cctcgtgaga
      961 agttacaaca aagcacttaa aaagaatgca ccctatccta tatttgcaat aaagaatggt
     1021 cccaaatctc acatcggtcg tcacttgatg acatctttcc tgtctatgaa gggcctcacc
     1081 gaactcacaa atgtagttgg gaactggtcc gataaaaggg cttcagctgt agctcgaact
     1141 acatatacac atcagataac cgctatacca gaccactatt ttgctcttgt ttccagatat
     1201 tacgcctacg accctatctc caaggagatg atagccttga aagatgaaac taatccaata
     1261 gaagaatggc aacacatcga acaactaaaa ggaagcgctg aagggagtat tcgataccct
     1321 gcttggaacg gcataattag tcaggaggtt cttgactatt tgtcatctta catcaaccgt
     1381 aggatttagc tttgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg
     1441 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1501 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1561 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1621 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1681 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
     1741 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     1801 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     1861 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     1921 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
     1981 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     2041 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     2101 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     2161 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     2221 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     2281 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     2341 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     2401 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     2461 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     2521 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     2581 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
     2641 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2701 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2761 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2821 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2881 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2941 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3001 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3061 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3121 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3181 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3241 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3301 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3361 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3421 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3481 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3541 cgcagcga
//
