LOCUS       sequence-391075-        4088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-PhiC31-9
FEATURES             Location/Qualifiers
     source          1..4088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             91..1905
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             1906..1926
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1968..1986)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2054..2158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2380..3171
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3342..3930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3831..3850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4084..4088,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggacactt atgctggtgc ctacgatcgt
      121 caatctagag agagagaaaa ttcctctgca gcatccccag ctacacagag gtcagcaaat
      181 gaggataagg cagccgatct tcagcgtgag gtcgagcgag atggaggtag gtttagattt
      241 gtgggacact tttctgaggc tcccggcact tctgcattcg gaaccgctga acgacctgag
      301 tttgagagaa tcctaaacga gtgtagagct ggacgactta atatgataat cgtgtatgat
      361 gtttcacgtt tttccaggct caaggtgatg gatgcaatcc caattgtcag cgaactcttg
      421 gcccttgggg tcaccattgt gtccactcaa gagggggtat tccgtcaggg caacgtaatg
      481 gatctgatac atctgataat gcgacttgat gcctctcaca aggagtcatc cctcaagtcc
      541 gctaagatat tagatacaaa gaacctacaa cgagaactag gaggctacgt gggtggaaaa
      601 gcaccatatg gatttgagct ggtatctgaa actaaagaaa ttacaaggaa tggtcgaatg
      661 gtcaacgtcg tcattaacaa actcgctcac agcacaacac cattgacagg tccatttgag
      721 ttcgaaccag atgttatccg atggtggtgg cgtgagatca agactcacaa gcacctaccc
      781 tttaaaccag gttcccaggc cgccatacac cccgggagta tcacaggctt gtgcaagcga
      841 atggacgccg acgctgttcc taccaggggg gagactatag gaaaaaaaac agcttccagt
      901 gcctgggacc ccgcaaccgt gatgagaatt ttaagagatc ccaggatagc aggctttgca
      961 gcagaggtaa tttataaaaa gaaacctgat ggtactccca ctactaaaat cgagggatat
     1021 cgtattcagc gagatcccat aaccctgcgt cccgtcgagt tggattgcgg gccaatcatc
     1081 gaacctgccg agtggtatga actgcaggcc tggttagacg gtcgagggag agggaaaggt
     1141 cttagcaggg ggcaagcaat cctctctgca atggataaac tatactgcga atgcggcgca
     1201 gtaatgactt ctaaacgtgg agaagaaagt atcaaagatt cttataggtg caggcgtcgt
     1261 aaggtagtcg atccttctgc tcccgggcaa catgaaggga cctgcaacgt atcaatggca
     1321 gctcttgata aattcgtagc cgaaaggatc ttcaacaaaa tccgtcacgc tgagggcgat
     1381 gaagaaacct tggctctttt atgggaagca gcaaggaggt ttggtaagtt aacagaggca
     1441 cctgagaagt ctggagagag ggctaatctt gtcgctgaac gagctgacgc acttaatgcc
     1501 ttggaggagc tgtacgagga tcgagctgcc ggggcctacg atggcccagt cgggagaaaa
     1561 cactttcgta aacagcaagc cgcactgaca ttaaggcagc agggtgctga ggagagacta
     1621 gcagagttgg aagcagccga agctcccaag ctgccactgg atcaatggtt ccccgaagat
     1681 gcagacgccg acccaactgg ccctaagagc tggtggggga gggcaagcgt cgatgacaag
     1741 agggtctttg tggggctatt tgtagacaaa atcgttgtga ccaaaagcac aaccggcagg
     1801 ggacagggaa ctcccatcga aaagcgagct tctattactt gggctaagcc accaaccgac
     1861 gacgatgagg acgatgcaca ggacggaaca gaagatgtag cagcaccaaa gaaaaagcga
     1921 aaggtatagc tttgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg
     1981 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2161 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2221 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
     2281 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     2341 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     2401 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     2461 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
     2521 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     2581 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     2641 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     2701 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     2761 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     2821 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     2881 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     2941 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     3001 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     3061 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     3121 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
     3181 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3241 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4021 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4081 cgcagcga
//
