LOCUS       sequence-391076-        3743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-R-9
FEATURES             Location/Qualifiers
     source          1..3743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1561..1581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1623..1641)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1709..1813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2035..2826
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2997..3585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3486..3505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3739..3743,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggctctca ccaaagacac agaaattagt
      121 acaataaacc gtcaaatgtc agacttcagc gagctatcac agatcttacc actccaccaa
      181 ataagcaaaa tcaaagatat actggagaac gaaaatccac ttccaaaaga gaagcttgcc
      241 agccacctca ctatgattat actaatggca aaccttgcat cccagaagcg aaaagatgtt
      301 ccagtgaaaa ggagcacatt tttgaaatat cagcgaagta tcagtaaaac actgcagtat
      361 gatagctcca ccaaaactgt ctccttcgag tatcacttga aagatccatc caaattgatt
      421 aagggcctag aagatgtagt aagcccttat aggttcgtgg ttggggtaca cgaaaaaccc
      481 gacgatgtaa tgtcccatct cagtgctgtc cacatgcgaa aggaagcagg acgtaagagg
      541 gatctgggga ataagatcaa tgatgaaata actaaaatag cagagacaca agagactatt
      601 tggggattcg tgggcaaaac tatggacctt atcgaggcac gaacaacccg accaacaact
      661 aaggcagctt ataacctttt gttacaagcc acattcatga actgttgtag agctgatgac
      721 ctcaaaaaca cagatattaa gacttttgag gtaatccccg ataaacacct gggaaggatg
      781 ctacgtgcat tcgtgcccga aaccaaaacc ggtaccaggt tcgtatattt cttcccttgc
      841 aaaggaaggt gtgatcctct cttggccctg gatagctact tacagtggac cgaccccatc
      901 cccaaaacca gaactaccga tgaagatgca cgatatgatt accagctctt gagaaatagt
      961 ttgcttggca gttacgatgg ctttatatcc aagcagagcg acgagtctat ttttaagatc
     1021 cccaacgggc ctaaagctca ccttggcagg cacgtaacag catcatactt gagtaacaac
     1081 gagatggata aggaggccac tttatacggg aattggtcag ccgcacgtga agagggtgtg
     1141 agtcgagttg ccaaagctcg ttatatgcat accatcgaga aatccccccc aagctatttg
     1201 ttcgcatttt tgagcgggtt ttacaatatc actgctgaaa gggcttgcga gttagtagac
     1261 ccaaacagca acccctgcga acaagacaag aatatcccca tgatttctga catagagaca
     1321 ctaatggcac gatacggtaa aaacgccgag attattccta tggacgttct cgtattcctg
     1381 agttcatacg caaggttcaa gaataacgaa ggtaaggagt ataaactcca agctcgttca
     1441 agtcgtggag ttccagattt tccagataat ggtagaactg ccctgtataa tgccttaact
     1501 gccgcacacg taaaaagaag gaaaatatca atcgtggtcg gtaggtctat tgacacttca
     1561 ccaaagaaga agcgaaaagt atagctttga gaccacgaag tggctcttca gtggacgaaa
     1621 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1681 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1801 aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     1861 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct
     1921 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     1981 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg
     2041 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     2101 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     2161 aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     2221 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     2281 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     2341 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     2401 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     2461 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     2521 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     2581 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     2641 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     2701 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     2761 gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc
     2821 aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2881 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2941 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3001 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3061 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3121 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3181 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3241 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3301 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3361 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3421 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3481 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3541 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3601 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3661 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3721 agccgaacga ccgagcgcag cga
//
