LOCUS       sequence-391077-        3710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-Integrase4-9
FEATURES             Location/Qualifiers
     source          1..3710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             97..117
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1590..1608)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1676..1780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2002..2793
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2964..3552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3453..3472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3706..3710,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggccccta agaagaagag gaaagttatg
      121 atcacaaccc gtaaggtagc catctacgtt cgtgtgtcaa ccactaatca ggccgaggag
      181 gggtacagca tacaagggca aatagattcc ctgataaaat attgtgaagc aatgggctgg
      241 attatctatg aagaatacac agacgcaggg ttcagtggtg gaaaaattga cagacctgct
      301 atgagcaaac tgattaccga cgccaagcac aagagatttg atacaattct cgtgtacaaa
      361 ttggacaggc tatccaggtc agtgagagat acactctatc tcgtcaaaga tgtttttaac
      421 cagaataata tccacttcgt gtctttacag gaaaatatcg acacctctag tgcaatgggg
      481 aatctcttct taacacttct atcagcaatt gccgaatttg agcgtgagca gataaccgag
      541 agaatgacta tggggaaaat tggtcgtgcc aagagcggca aaacaatggc ctggacttac
      601 actccctttg gatatgacta caacaaagaa aaaggggaat taatactgga cccagccaaa
      661 gctcctatag tcaaaatgat atacactgat tacctgaagg gaatgtctat acagaaaatc
      721 gtggataaat taaataaaat ggattacaac ggcaaagact gcacctggtt tccacacgga
      781 gtgaaacatc ttttggataa cccagtctac tacgggatga ctcgatacaa taataaattg
      841 tttccaggaa accaccagcc tataattaca aaggaattgt tcgataaaac ccaaagagag
      901 aggcaaagga gaagattggg gatagaagaa aaccactata caataccctt tcaggcaaaa
      961 tatatgctta gtaagttctt gcgttgccgt cagtgtggtt cccgaatggg actggaattg
     1021 ggaaggccca ggaagaagga agggaaaagg tctaaaaagt actattgtct gaactcaaga
     1081 cccaaacgaa cagctagttg cgacacccca ctatatgacg ctgaaacctt ggaggactac
     1141 gttctccatg aaatcgcaaa gatacaaaag gatcctagta ttgcaagcag gcaaaagcat
     1201 atcgaggacc acgagctgaa gtataaacgt gagagaattg aagccaatat aaacaagacc
     1261 gtcaaccaac tttctaagct caacaactta taccttaacg atctcatcac actggaggac
     1321 ctaaaaacac agaccaatac tcttattgct aaaaagaggc ttttagagaa tgagttagat
     1381 aaaacttgtg acaatgacga tgagctggat cgtcaggaaa caattgcaga tttcttagcc
     1441 ctcccagatg tatggaccat ggactacgaa ggacagaagt atgctgtaga gctgctcgtc
     1501 caaagagtta aggtagatcg tgacaatata gatatccact ggaccttcta gctttgagac
     1561 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1621 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1741 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1801 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1861 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1921 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1981 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     2041 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2101 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     2161 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2221 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2281 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2341 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2401 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2461 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2521 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2581 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2641 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2701 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     2761 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     2821 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
//
