LOCUS       sequence-391081-        8720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses APP695 with an HA tag an N-terminal for single molecule 
            tracking and other measusers in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Capitini C, Bigi A, Parenti N, Emanuele M, Bianchi N, Cascella R, 
            Cecchi C, Maggi L, Annunziato F, Pavone FS, Calamai M
  TITLE     APP and Bace1: Differential effect of cholesterol enrichment on 
            processing and plasma membrane mobility.
  JOURNAL   iScience. 2023 Apr 11;26(5):106611. doi: 10.1016/j.isci.2023.106611.
            eCollection 2023 May 19.
  PUBMED    37128606
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-04-11"; volume: "26(5):106611. doi"; pages: " 
            10.1016/j.isci.2023.106611. eCollection 2023 May 19"
COMMENT     Sequence Label: HA-APP-mGFP
FEATURES             Location/Qualifiers
     source          1..8720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1086..1112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1398..1409)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             1889..1909
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             3177..3890
                     /codon_start=1
                     /product="monomeric green fluorescent protein, also known 
                     as mTagGFP"
                     /label=TagGFP2
                     /note="mammalian codon-optimized"
                     /translation="MSGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIK
                     FICTTGKLPVPWPTLVTTLCYGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIQFQDDG
                     KYKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYIRPDKANNGLEA
                     NFKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSTQTKISKDRNEARDHMVLLE
                     SFSACCHTHGMDELYR"
     primer_bind     complement(3925..3941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3952..4574
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4151..4170)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4598..4618)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4603..4932
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4783..4918
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4999..5793
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5053..5072)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5663..5682
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5967..6088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6004..6023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6058..6077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6137..6153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6150..6172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6161..6177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6185..6191,6192..6209,6210..6215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6230..6251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6368..6385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6539..7127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6619..6638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7298..8089,8090..8158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7921..7940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8159..8263)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8290..8718
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8377..8396)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8587..8608
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gctgcccggt ttggcactgc tcctgctggc cgcctggacg gctcgggcgg
     1081 ctagctatcc gtacgacgta ccagactacg caggcgcgcc tctggaggta cccactgatg
     1141 gtaatgctgg cctgctggct gaaccccaga ttgccatgtt ctgtggcaga ctgaacatgc
     1201 acatgaatgt ccagaatggg aagtgggatt cagatccatc agggaccaaa acctgcattg
     1261 ataccaagga aggcatcctg cagtattgcc aagaagtcta ccctgaactg cagatcacca
     1321 atgtggtaga agccaaccaa ccagtgacca tccagaactg gtgcaagcgg ggccgcaagc
     1381 agtgcaagac ccatccccac tttgtgattc cctaccgctg cttagttggt gagtttgtaa
     1441 gtgatgccct tctcgttcct gacaagtgca aattcttaca ccaggagagg atggatgttt
     1501 gcgaaactca tcttcactgg cacaccgtcg ccaaagagac atgcagtgag aagagtacca
     1561 acttgcatga ctacggcatg ttgctgccct gcggaattga caagttccga ggggtagagt
     1621 ttgtgtgttg cccactggct gaagaaagtg acaatgtgga ttctgctgat gcggaggagg
     1681 atgactcgga tgtctggtgg ggcggagcag acacagacta tgcagatggg agtgaagaca
     1741 aagtagtaga agtagcagag gaggaagaag tggctgaggt ggaagaagaa gaagccgatg
     1801 atgacgagga cgatgaggat ggtgatgagg tagaggaaga ggctgaggaa ccctacgaag
     1861 aagccacaga gagaaccacc agcattgcca ccaccaccac caccaccaca gagtctgtgg
     1921 aagaggtggt tcgagttcct acaacagcag ccagtacccc tgatgccgtt gacaagtatc
     1981 tcgagacacc tggggatgag aatgaacatg cccatttcca gaaagccaaa gagaggcttg
     2041 aggccaagca ccgagagaga atgtcccagg tcatgagaga atgggaagag gcagaacgtc
     2101 aagcaaagaa cttgcctaaa gctgataaga aggcagttat ccagcatttc caggagaaag
     2161 tggaatcttt ggaacaggaa gcagccaacg agagacagca gctggtggag acacacatgg
     2221 ccagagtgga agccatgctc aatgaccgcc gccgcctggc cctggagaac tacatcaccg
     2281 ctctgcaggc tgttcctcct cggcctcgtc acgtgttcaa tatgctaaag aagtatgtcc
     2341 gcgcagaaca gaaggacaga cagcacaccc taaagcattt cgagcatgtg cgcatggtgg
     2401 atcccaagaa agccgctcag atccggtccc aggttatgac acacctccgt gtgatttatg
     2461 agcgcatgaa tcagtctctc tccctgctct acaacgtgcc tgcagtggcc gaggagattc
     2521 aggatgaagt tgatgagctg cttcagaaag agcaaaacta ttcagatgac gtcttggcca
     2581 acatgattag tgaaccaagg atcagttacg gaaacgatgc tctcatgcca tctttgaccg
     2641 aaacgaaaac caccgtggag ctccttcccg tgaatggaga gttcagcctg gacgatctcc
     2701 agccgtggca ttcttttggg gctgactctg tgccagccaa cacagaaaac gaagttgagc
     2761 ctgttgatgc ccgccctgct gccgaccgag gactgaccac tcgaccaggt tctgggttga
     2821 caaatatcaa gacggaggag atctctgaag tgaagatgga tgcagaattc cgacatgact
     2881 caggatatga agttcatcat caaaaattgg tgttctttgc agaagatgtg ggttcaaaca
     2941 aaggtgcaat cattggactc atggtgggcg gtgttgtcat agcgacagtg atcgtcatca
     3001 ccttggtgat gctgaagaag aaacagtaca catccattca tcatggtgtg gtggaggttg
     3061 acgccgctgt caccccagag gagcgccacc tgtccaagat gcagcagaac ggctacgaaa
     3121 atccaaccta caagttcttt gagcagatgc agaacacgcg tacgcggccg ctcgagatga
     3181 gcgggggcga ggagctgttc gccggcatcg tgcccgtgct gatcgagctg gacggcgacg
     3241 tgcacggcca caagttcagc gtgcgcggcg agggcgaggg cgacgccgac tacggcaagc
     3301 tggagatcaa gttcatctgc accaccggca agctgcccgt gccctggccc accctggtga
     3361 ccaccctctg ctacggcatc cagtgcttcg cccgctaccc cgagcacatg aagatgaacg
     3421 acttcttcaa gagcgccatg cccgagggct acatccagga gcgcaccatc cagttccagg
     3481 acgacggcaa gtacaagacc cgcggcgagg tgaagttcga gggcgacacc ctggtgaacc
     3541 gcatcgagct gaagggcaag gacttcaagg aggacggcaa catcctgggc cacaagctgg
     3601 agtacagctt caacagccac aacgtgtaca tccgccccga caaggccaac aacggcctgg
     3661 aggctaactt caagacccgc cacaacatcg agggcggcgg cgtgcagctg gccgaccact
     3721 accagaccaa cgtgcccctg ggcgacggcc ccgtgctgat ccccatcaac cactacctga
     3781 gcactcagac caagatcagc aaggaccgca acgaggcccg cgaccacatg gtgctcctgg
     3841 agtccttcag cgcctgctgc cacacccacg gcatggacga gctgtacagg tccggactca
     3901 gataagttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     3961 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     4021 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     4081 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     4141 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     4201 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     4261 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     4321 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     4381 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     4441 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     4501 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     4561 atgcctgttg aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt
     4621 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4681 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4741 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4801 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4861 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4921 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     4981 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     5041 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5101 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5161 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5221 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5281 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5341 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5401 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5461 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5521 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5581 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5641 gccgaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5701 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5761 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5821 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5881 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5941 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     6001 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6061 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     6121 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7201 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7261 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7561 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8101 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8161 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8221 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8281 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     8341 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     8401 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8461 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8521 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8581 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8641 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8701 aaaaatttaa cgcgaatttt
//
