LOCUS       sequence-391082-       12314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid for Hb9 GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH
  TITLE     Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor 
            neurons derived from human embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2008 Dec 4;3(6):649-57.
  PUBMED    19041781
REFERENCE   2  (bases 1 to 12314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2008-12-4"; volume: "3(6)"; pages: "649-57"
COMMENT     Sequence Label: Lenti-Hb9-GFP
FEATURES             Location/Qualifiers
     source          1..12314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        458..656
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             674..854
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    901..1026
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1519..1752
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1937..1981
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2233..2349
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5981..6019
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(6051..6053,6054..6056,6057..6770)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6051..6053,6054..6056,6057..6767)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6096..6117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6357..6376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6704..6725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6963..7551
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7016..7036)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7434..7445)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7958..8138
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8164..8181)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8170..8394
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8440..8868
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8527..8546)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8737..8758
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8877..8897)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8882..9211
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9062..9197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9124..9143
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9259..9306
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9325..9699
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9829..9950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9866..9885)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9920..9939
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9999..10015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9999..10015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10012..10034)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10023..10039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10047..10053,10054..10071,10072..10077))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10092..10113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10230..10247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10401..10989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10481..10500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11160..11951,11952..12020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11783..11802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12021..12125)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12200..12219)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
       61 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      121 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
      661 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      721 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      781 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      841 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
      901 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      961 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     1021 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     1081 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1141 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1201 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1261 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1321 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1381 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1441 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1501 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1561 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1621 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1681 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1741 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1801 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     1861 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1921 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1981 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2041 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2101 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2161 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2221 gaacggatca acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2281 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt
     2341 caaaatttta tcgatgcggc cgctcgactc gatcctgtct ccctggccag gagtctgggg
     2401 tccgcagata tattacaaca aaattactaa ttggaaaatg aaattgtcaa taaagcacac
     2461 cccacgcgtg tactatatct ttaaatatta tgatgtctta ttggtcccat ttccttacaa
     2521 taatttattg caataaaaca gacactgcag gtccccttaa attgtctttt cataatgaat
     2581 aaatttaagc aggctaatta atatataaac tagctcaatt tgtcaagttg atttgtattt
     2641 tagttaattg tgaaagtaat taccacatgg tcaaattaac agctttctgg aaatgaccaa
     2701 gcctgaggtt ttatttcctt cctgggtgaa gaaaattcat ttttccaagc tcttgatgtg
     2761 atgaataaaa gtcataaatc tgggtgattg gtgcaggcag agtctaaatg gcttcatatt
     2821 tcattttagg tttaatagaa atattcatgc tctgttttaa tgaaattaaa ttgaaggggg
     2881 atggggctct ggaggcggcc ctggctgggg gcttaaaggg acagtgcact tttcctttct
     2941 tcatgcaagg gccagggcta gaggtccctg gactcagtca agaagtggag agagctgagg
     3001 gaggcctggg aggcccttat tctgtaccca gtgtagcaca cctgcagccc tgattggggg
     3061 gggggggcgg gggcaattca caccctgtat tctcaccagg ctaaagtttc ttctatctgg
     3121 ttccttctct ccaaaaaatc agtcagaagg gacctccctt ggctgagagt agaagtgagg
     3181 tggtcagcgt gctgcagaca agcctgtcct ccatctgcct aaggggtctg atatcctaca
     3241 ccaagccccc cctacccctt ctggagacac ataggagata agcattttca aaaccaccat
     3301 ggcactctcc tgttctcccc tgcacccctt cctcccagac tccatcctgc ctgaggatga
     3361 tttggtccaa cttgaataat agagtactct ctttccacat ctttccacat cggaaagagt
     3421 agtccgggat ccagtccacc tgcagccctg attaaaggaa ggaaggaaga ctagaggcgc
     3481 agttctacct attatactta ttttaaggtt cccaggtcag agatgcctca tggacatttc
     3541 tccctaaggc aggtcctgac cagccctccc cctgaatggt gctgatctgt gacttgttgc
     3601 ttctattgaa caaatacagc tttgatttgt ttccagccag agtggttagc tgatgaattg
     3661 acaaaaacta atcagcttta ttgggaaaca ggtttaaggg cacggacgtg tcaataacgc
     3721 tcagcctgac cccctcttcc attagctagg caggctgatt agagtaagaa gaccctctcg
     3781 ccgattgcac cgaaattgct ttcataaact cacaatatta gcagtcgatg tgagcagcta
     3841 atttagagaa gacatccagg cccgtcacat cgatcatcgg gtttatgtat cattatctca
     3901 tttacagaat gtcgcagtgt aatacatgtt tttggcaata ggcttggccg ctcgccggag
     3961 ccgcgcgctg ttcctttcgg ccacactgca ggggacttcc tcaacaatct acgatgtctc
     4021 ttcgcgtgaa caaggaggca ctttgcacac atatgcacac ttgcgcccag gctctcagtg
     4081 cgcgctcagt gcacacatgc acgtggacac atgctccgac atactacctg agctgccttt
     4141 ccttctcatg cggggatgct ggcattgctc tctcctgcga gcccctggtc tgaagcccaa
     4201 tgccggacgc tcaccaacct tggagtcaac ctcggagagc tcaaggttcg gttctgtcac
     4261 cagcatacag agatggtggg tggcggcgca atcagccacc ttctctcaag gctttggtcg
     4321 cccagttggc gggtgtccag aaatccacag gcctgcgaag gtccttctcc gccctcccag
     4381 gctccagctt gacctgccct gccctgggcg ggcctttctc gcaacacttc caggctcagc
     4441 cagatgcgcc tggtcttagg gagtgggcca cctgttcctt tgggtaatta ttttaatgta
     4501 aggggtctac ttcaggcctc ctgcagtgac agacggcagc ggagctgcaa agcgctctca
     4561 gcctttcagt ggaatttccc agatgggcca aggggaggcc agggcgggtc accaggggcc
     4621 ctttaacgag ttcattagcc aaattatctt gcctgcagca aggaccccag agactgtagg
     4681 gagagtaggg gagaaggtgg gtctttctcc ctccctgcta gtctgcaaga acagttgtgg
     4741 gaaggaaaga gaaaggacat cctccccaaa ggggcctagg aacactaaag gcaaagatgg
     4801 catctctgat gtgacttatg cgccagacca gcgaggtggt ggagtggtgg gaaggaatca
     4861 taatgattag gaaacacgga gattctaaca accagggcac tgacccatat tgtggaacaa
     4921 acctagtgca gaaatcaaag tcagagcccc tggaacttta ttctgtgctt cttaagcaaa
     4981 gaacctggac atcttggagc cttggtctgt ccgtccatct gtagagtaga aggtaggtgt
     5041 gtttccttcc acaatttgtg agatcactgc cctaagaaaa gcaactctgg gaagagcttc
     5101 tggcatgacc ctgttgggtc cttcgggccc ccttactcct gtggtaggag gctttggcag
     5161 gttgaggtga gcctgatcct agatgcctgt gctggaaaat gaacccaact ctcacctgtc
     5221 gtcgcgcccc ccacccccac cccacagctg tgaggcgctg ctctggaggt agctcagcat
     5281 ccccgcccct cctccttgtc ctcactctgc ctcctgtagt gttagcttct gctacctcag
     5341 caagtagtga gtgaatagac caattagcca ggacttcctg ccattatctg ctgtagactt
     5401 gggcattagt acgccctgat tcaccataat ttcaagtcag aacacactaa aaaattcact
     5461 caccgaggca ttaaaatgtt cccagacaac gtctgtgttt gtgtaactgg gtttcttccc
     5521 ttcgtgtttg atgcacacca gcatcatata ggtcagtggt ggagtccagg ctaggcagac
     5581 aatttttgtc gctttttgtt tgtttttata tttttaaaac atgaattgca ttgtttcccg
     5641 gtggttgtaa actttgtttc tggggcttca gtattgccag tgtaatcttc ttctgcttga
     5701 tcaggggcaa acaagtgact tagaaagttg tgggagtgcc ctaagaacag tagccagagc
     5761 ctttacagag ccaggctttg tgtcctaaac gtacatcgcc ccagctatgg gaagctgggt
     5821 tatcaccttc aaagcagacc ctttctgtgg atgacttacc aagccctgca ggtgggtagt
     5881 ggaggtgggg agagggctgc acagcctgct tggagcttgc ttctgttctt tgtcctgccc
     5941 atgatatgaa gctcctatac aatgaatttg cagtaagatc ggtaggcgtg tacggtggga
     6001 ggtctatata agcagagctg gtttagtgaa ccgcagatcc ggcgcgcccc atggtgagca
     6061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     6121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     6181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     6241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     6301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     6361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     6421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     6481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     6541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     6601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     6661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     6721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccaat
     6781 tcggatccgt ttaaactcta gaggatccac cggtggccgc ctgggcccgt taacgtctcg
     6841 agtttaaacc tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6901 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     6961 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7021 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7081 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7141 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7201 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7261 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7321 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     7381 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7441 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7501 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     7561 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7621 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     7681 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     7741 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     7801 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     7861 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     7921 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     7981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8041 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8101 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat
     8161 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8221 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8281 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8341 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     8401 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     8461 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8521 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8581 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8641 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8701 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8761 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8821 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     8881 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8941 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9001 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9061 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9121 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     9181 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     9241 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     9301 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     9361 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     9421 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     9481 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     9541 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     9601 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     9661 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     9721 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9781 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     9841 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9901 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     9961 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    10021 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10081 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10141 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10201 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10261 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10321 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10381 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10441 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10501 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10561 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10621 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10681 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10741 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10801 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10861 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10921 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10981 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11041 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11101 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11161 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11221 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11281 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11341 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11401 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11461 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11521 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11581 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11641 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11701 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11761 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11821 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11881 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11941 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12001 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12061 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12121 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
    12181 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
    12241 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
    12301 ggcaaggctt gacc
//
