LOCUS       sequence-391083-        3776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-Bxb1-9
FEATURES             Location/Qualifiers
     source          1..3776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1594..1614
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1656..1674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1742..1846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2068..2859
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3030..3618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3519..3538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3772..3776,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggggcgtg ctctagtggt tataaggctc
      121 tcccgagtga ctgatgctac tacaagtcca gaaaggcaat tagagagttg tcagcaattg
      181 tgcgctcaga gaggttggga cgtggttggc gtggcagagg acctagatgt gagtggggct
      241 gttgacccct ttgatcgaaa aagaaggcca aatctagcca gatggctcgc ttttgaggag
      301 caaccctttg atgtcatagt cgcctaccgt gtcgacagac ttactcgttc aatccgacac
      361 cttcagcaac tagtccactg ggcagaggat cacaaaaagc tagtagtaag tgcaactgaa
      421 gcccacttcg acacaactac ccccttcgca gctgtagtaa ttgccctgat ggggacagtt
      481 gcccaaatgg aactggaggc tatcaaagaa agaaacaggt cagcagcaca ctttaatata
      541 agagctggga aatatagagg gagtctgccc ccctggggat atctcccaac aagagtagac
      601 ggcgaatgga ggttggtgcc tgatccagtt cagagagaac gaattctaga ggtttatcac
      661 agggtagtgg acaaccatga gccactacac ttggtcgccc acgacctaaa cagacgaggg
      721 gttttgagcc ccaaggacta ttttgcccag cttcaagggc gagaacctca ggggcgagag
      781 tggtccgcaa ccgcccttaa acgtagcatg ataagtgaag ccatgcttgg ctacgccacc
      841 ctaaatggta aaaccgtcag agatgacgac ggcgctcctc tggtcagggc cgaacccata
      901 ttgaccaggg agcagttaga agccttaaga gcagaactag tcaaaacttc aagggccaaa
      961 cccgcagtct caacaccctc acttctgttg agggtactat tttgcgccgt ttgcggtgaa
     1021 cccgcttata agtttgcagg gggtggccgt aaacatccac gataccgttg cagatctatg
     1081 ggatttccca aacactgcgg gaatggtact gttgcaatgg ctgaatggga tgcattttgt
     1141 gaagaacaag ttcttgattt gctgggtgat gctgaacgtt tagaaaaggt gtgggtcgca
     1201 ggctctgact ccgccgtaga attggcagaa gtgaacgctg aactagttga cctaacttcc
     1261 ttaataggat ctcctgccta ccgtgctggg agtccccaaa gggaagctct tgacgcaagg
     1321 atagctgcat tagcagcaag acaggaggaa ctagaaggat tggaagctag accatccggc
     1381 tgggagtggc gagagacagg acagcgtttc ggagattggt ggcgagaaca agacaccgca
     1441 gccaaaaata cctggctgcg atctatgaat gtccgtctta ccttcgatgt ccgtggcgga
     1501 ttaacacgaa ccattgactt tggtgacctc caggagtacg aacagcactt aagactgggg
     1561 tccgtcgttg aacgtttaca tacaggaatg tcccccaaga agaagaggaa agtttagctt
     1621 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
     1681 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
     1861 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     1921 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1981 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     2041 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     2101 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     2161 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
     2221 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2281 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2341 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2401 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2461 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2521 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2581 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2641 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2701 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2761 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     2821 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     2881 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2941 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3001 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3061 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcga
//
