LOCUS       sequence-391088-        3878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-ELuc-9
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1758..1776)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1844..1948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2170..2961
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3132..3720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3621..3640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3874..3878,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggaacgag agaagaatgt agtatacggg
      121 ccagagccca aacatcctct cggtaacttt accgctggtg aaatgctata taacgccctg
      181 cataagcact cccacatccc ccaagctatc ctagacgtta tgggcaacga gagtctttct
      241 tatcaggaat ttttcgacac tacagtaaaa ctggggcaga gtttgcagaa ttgtggctac
      301 aaaatgaatg atgtagtttc tatctgtgcc gagaacaaca agcgattctt tatacctatt
      361 atctcagcct ggtatattgg aatggtagtt gctcccgtca acgaggacta catcccagat
      421 gagctgtgta aggtgactgg catatccaag cccatcctgg tatttactac tagaaagatc
      481 cttccaaagg tgttagaggt taaagacaga accaactata taaagcgaat tataatatta
      541 gattctgagg agaatctact gggatgtgag agtctccaca actttatgag tcgttattca
      601 gataacaatc tccagacctt taaaccttta cactatgatc ctgtagatca ggttgctgcc
      661 attctttgct ctagtggtac cacagggcta ccaaagggtg taatgcaaac ccatagaaat
      721 atctgcgtcc gacttactca tgcatcagat cctagggtgg ggactcaact aattcctgga
      781 gtcagcgtac tcgcttatct gccattcttt catgcattcg gcttttctat taatctgggt
      841 tattttatgg tggggctgag agtcgtgatg ctacgaaggt ttaatcaaga agtgttcctg
      901 aaagccatcc aagactatga ggtaaggtct gtcataaatg tacctagtac cattctgttt
      961 ctgtctaaat ccccattagt ggacaagtac gacctgtcta ctttggcaga gctatgttgt
     1021 ggtgcagcac ctttagccaa ggaggttgcc gagattgcag tcaaaagatt aaacctgcca
     1081 ggaatcagat gtggttatgg gcttacagag tccacttccg ccaacattca tactttgcat
     1141 aacgagttca agtcaggtag tttgggaaaa gttaccccct acatggccgc aaaaataatc
     1201 gatcgtaata caggcgaggc actcggaccc aaccaagtag gtgagctgtg catttggggc
     1261 ccaatggtta caaaaggcta tgtcaacaac ccccaagcaa ccaaagaagc aatcgacgac
     1321 gacggctggt tacatagcgg agacttcggt tactatgatg aggacgaata cttctatatt
     1381 gtggaccgat ataaggaact cataaaatac aaaggttatc aagttgcccc tgtagagtta
     1441 gaggagattt tactgcaaca ccccggaata cgtgacgttg ccgtggttgg cattccagat
     1501 attgaagctg gagaactccc tgctgggttt gttgtcaagc agcctggcgc acaattgacc
     1561 gcaaaggagg tgtatgattt tctcgcccag cgagtttcac actctaagta tcttcgaggt
     1621 ggagtcagat ttgtcgactc tatcccaagg aacgtgaccg ggaagatcag tcgaaaggaa
     1681 ttgcgtgaag cccttatgga gaaggcatcc aagctctagc tttgagacca cgaagtggct
     1741 cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     1801 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1921 gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac
     1981 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2041 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     2101 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     2161 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     2221 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     2281 gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg
     2341 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     2401 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     2461 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     2521 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     2581 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     2641 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     2701 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     2761 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     2821 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     2881 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc
     2941 accaaggtag tcggcaaata actgtcagac caagtttact catatatact ttagattgat
     3001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3841 gataccgctc gccgcagccg aacgaccgag cgcagcga
//
