LOCUS       sequence-391089-        3903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-FLuc-9
FEATURES             Location/Qualifiers
     source          1..3903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1783..1801)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1869..1973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2195..2986
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3157..3745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3646..3665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3899..3903,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggaagatg ctaagaatat taagaaggga
      121 ccagctcctt tctacccact cgaagatgga acagccggtg aacagttaca caaggctatg
      181 aaaagatatg ctcttgttcc tggtactatc gcattcacag atgctcatat cgaggtggat
      241 ataacttatg ctgagtactt cgaaatgtct gttaggctcg ctgaagctat gaagagatac
      301 ggattaaata caaaccacag gattgttgtg tgtagtgaaa attctttgca atttttcatg
      361 cctgttcttg gagctttgtt catcggtgtg gctgttgcac cagctaatga tatatataac
      421 gagagagaac ttcttaattc tatgggaatt tctcaaccta ctgttgtgtt cgtgtctaag
      481 aaaggacttc aaaagatttt gaacgttcag aagaaacttc caattatcca gaagattatt
      541 attatggatt caaagacaga ttaccaaggt ttccagtcaa tgtacacctt tgtgactagt
      601 catttgcctc ctggattcaa tgagtacgat ttcgttccag aaagttttga tagagataag
      661 actattgctc tcattatgaa ctcttcagga tctacaggcc ttcctaaggg tgtggcattg
      721 ccacatagaa ccgcttgtgt taggttctct cacgcaagag atcctatttt cggaaaccag
      781 ataattccag atacagctat cttgtcagtt gtgcctttcc atcacggatt tggaatgttc
      841 actacactcg gataccttat ttgtggtttt agggttgtgc tcatgtacag attcgaagag
      901 gaactctttt taagatcttt acaagattat aagatacagt cagcactctt agttccaacc
      961 cttttctctt tctttgctaa gtcaactttg atcgataagt acgatctttc taacttgcat
     1021 gagattgcaa gtggaggtgc tcctctttct aaagaagtgg gtgaagcagt tgctaagagg
     1081 tttcacttgc caggaatcag acaaggatat ggtcttacag agactacttc tgctatcttg
     1141 ataacccctg aaggagatga taaaccagga gcagttggaa aggttgttcc tttctttgaa
     1201 gctaaagttg tggatctcga tacaggaaag accttaggtg tgaatcagag gggagagctt
     1261 tgcgttagag gtcctatgat aatgagtgga tatgttaata acccagaagc aactaacgct
     1321 cttatcgata aagatggatg gttgcattca ggagatattg cttactggga tgaggatgaa
     1381 cactttttca tagtggatag gctcaagtca ttaattaagt ataagggata ccaagttgcc
     1441 cctgctgagc tcgaaagtat tcttttgcag catccaaaca tcttcgatgc aggagttgct
     1501 ggccttcctg atgatgatgc tggagagctc ccagctgcag ttgtggtttt agaacacgga
     1561 aagactatga cagagaaaga aattgtggat tacgttgcat ctcaagttac aaccgctaag
     1621 aaacttagag gtggtgtggt ttttgtggat gaggttccaa agggactcac tggtaaatta
     1681 gatgctagaa agatcaggga aattttgatc aaagcaaaga aaggaggaaa gattgctgtt
     1741 tgagctttga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
     1801 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1861 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1921 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
     1981 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2041 tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca
     2101 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     2161 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     2221 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2281 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga
     2341 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     2401 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     2461 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     2521 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     2581 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     2641 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     2701 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     2761 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     2821 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     2881 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     2941 tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt
     3001 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3901 cga
//
