LOCUS       sequence-391091-        3896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-RedFLuc-9
FEATURES             Location/Qualifiers
     source          1..3896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1776..1794)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1862..1966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2188..2979
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3150..3738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3639..3658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3892..3896,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggaaaaca tggaaaatga cgagaatatt
      121 gtggtagggc ccaaaccttt ctatcccatt gaagagggaa gcgccggaac tcaacttcga
      181 aagtacatgg aaagatacgc taagcttggc gctatcgcat ttaccaacgc agtgactggt
      241 gttgattact cttatgctga atacttggaa aaaagttgct gtttagggaa ggcactacaa
      301 aactacggac ttgtcgtcga tggaagaatc gccctgtgtt ccgagaattg cgaggagttc
      361 ttcattccag tcatagccgg cttattcatt ggagtcggtg tcgcacctac taacgaaatc
      421 tatactctcc gagaacttgt gcactcatta ggcatctcca agccaaccat cgtgttttct
      481 agcaagaaag gattggacaa agttatcacc gtccaaaaga ctgtaacaac cataaagaca
      541 atcgtcatac ttgattcaaa agtagattac cgaggctatc agtgcttaga cacattcatc
      601 aagaggaaca ccccaccagg ctttcaagcc tcttccttca agaccgttga ggtggaccga
      661 aaggagcagg tcgccctgat aatgaactca agcggatcta ctggacttcc taaaggggtc
      721 cagctaacac atgagaatac agtgactagg ttctcacacg cccgagatcc aatatacggg
      781 aatcaggtaa gccctggcac agcagtccta accgtagtcc ccttgcacca cggttttggg
      841 atgttcacca cactcgggta tttaatctgt gggttcaggg tcgtgatgtt gacaaaattc
      901 gacgaggaga catttcttaa aaccttacag gattataaat gtacttacgt tattttggtg
      961 ccaactcttt ttgctatcct taacaaaagt gagcttctga ataaatacga cttaagcaat
     1021 cttgtagaaa tcgcttcagg aggtgcccca ctctctaaag aagtggggga ggctgttgct
     1081 agacgtttta accttcctgg tgttagacaa ggatacggac taactgaaac cacatcagcc
     1141 atcatcatta ctcccgaggg cgacgataaa cccggtgcta gcggtaaggt tgtgccactt
     1201 ttcaaagcaa aagttataga cttggacaca aagaaatctt tagggccaaa taggcgaggt
     1261 gaagtatgcg taaagggccc aatgttaatg aaggggtatg ttaacaatcc tgaggccact
     1321 aaggagctta tcgacgagga aggctggctg cataccggtg acattggtta ttacgacgaa
     1381 gaaaaacact ttttcattgt tgacaggtta aaaagtttaa taaagtataa gggttaccag
     1441 gtgccccctg cagaactaga atctgtcctc ttgcaacatc catccatctt cgacgcaggg
     1501 gtagcaggag tccccgaccc agtagcaggc gagctacccg gcgctgtagt ggtgctagaa
     1561 agcggcaaga acatgaccga aaaggaagtt atggactacg ttgcttccca agtgtccaat
     1621 gcaaagcgtt tgcgtggtgg tgttaggttc gttgatgaag tgcccaaggg gttaacaggc
     1681 aagatcgacg gtagggctat acgagagatc ttgaagaagc cagtagctaa aatgtagctt
     1741 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
     1801 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1861 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1921 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
     1981 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     2041 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     2101 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     2161 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     2221 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     2281 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
     2341 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2401 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2461 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2521 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2581 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2641 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2701 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2761 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2821 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2881 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     2941 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     3001 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3061 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3121 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3181 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3241 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3301 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3361 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3421 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3481 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3541 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3601 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3661 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3721 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3781 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3841 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcga
//
