LOCUS       sequence-391092-        3185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-RLuc-9
FEATURES             Location/Qualifiers
     source          1..3185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1065..1083)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1151..1255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1477..2268
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2439..3027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2928..2947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3181..3185,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgacatcta aagtgtacga ccccgaacag
      121 agaaagcgaa tgattacagg gccacaatgg tgggccaggt gcaagcaaat gaatgtgtta
      181 gacagcttta tcaactacta cgatagtgaa aaacacgccg agaatgccgt tatattttta
      241 catggtaacg ctgcctcatc ttatctctgg cgacacgtag tcccccatat agagcccgta
      301 gccaggtgta ttattccaga cctcatcggc atgggtaaga gcggtaaatc tggcaacggg
      361 agttatcgtt tattagacca ttacaagtat ctgacagctt ggttcgagtt actaaacctt
      421 cctaaaaaga tcatatttgt cgggcacgac tggggcgctt gtttggcatt ccactacagt
      481 tacgaacacc aagataagat taaggcaatc gtccatgctg agtcagtcgt agacgtgata
      541 gagagttggg atgagtggcc agacatcgaa gaagatattg cactcattaa gagtgaggaa
      601 ggagagaaaa tggtactgga gaacaacttt ttcgtcgaaa ccatgcttcc ctctaaaatt
      661 atgaggaaat tggaacccga agagttcgct gcttatttag aaccctttaa agagaagggc
      721 gaagtccgaa ggcctactct ctcatggcca agagagattc cactagtcaa aggcggcaag
      781 ccagatgtag tacaaatagt gagaaactat aacgcttatt tacgagcaag tgatgatttg
      841 ccaaagatgt ttatagaaag cgatccaggc ttcttttcta acgcaatagt ggaaggtgct
      901 aagaagtttc caaacactga attcgtgaaa gttaaagggc tccatttcag tcaagaggac
      961 gctcctgacg agatgggtaa gtatataaaa tcatttgtcg agagggtcct caaaaacgag
     1021 caatagcttt gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct
     1081 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1201 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg
     1261 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     1321 tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag
     1381 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     1441 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
     1501 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     1561 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt
     1621 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     1681 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     1741 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1801 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1861 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     1921 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     1981 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     2041 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     2101 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     2161 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     2221 tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa
     2281 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3181 agcga
//
