LOCUS       sequence-391093-        6207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-Ruby-9
FEATURES             Location/Qualifiers
     source          1..6207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             91..4041
                     /label=RUBY
     CDS             1591..1647
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2482..2538
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(4087..4105)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4173..4277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4499..5290
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5461..6049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5950..5969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(6203..6207,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggatcatg cgaccctcgc catgatcctc
      121 gcgatctggt tcatcagctt ccacttcatc aagctgctgt tctcccagca gaccaccaag
      181 ctgcttccgc caggaccaaa gccgcttccg atcatcggca acatccttga ggtgggcaag
      241 aagccgcatc ggtccttcgc caacctcgcc aagattcacg gcccactcat ttccctcaga
      301 ctcggctctg tgaccaccat cgttgtgtcc tctgccgacg tggccaaaga gatgttcctc
      361 aagaaggatc acccgctctc caaccgcacg atcccgaata gtgttacagc cggcgaccac
      421 cacaagctca ccatgtcttg gctcccggtg tctccgaagt ggcgcaactt ccgcaagatt
      481 accgccgtgc atctgctctc cccacagaga ctcgatgcct gccagacatt caggcacgcc
      541 aaggtgcagc agctctacga gtacgttcaa gagtgcgccc agaaaggcca ggccgtggat
      601 attggcaagg ccgcctttac gaccagcctc aacctcctca gcaagctgtt cttcagcgtc
      661 gagctggcgc accacaagtc ccataccagc caagagttca aagagctgat ctggaacatc
      721 atggaagata taggcaagcc gaactacgcc gactacttcc cgattctcgg ctgcgttgac
      781 ccatctggca ttagaagaag gctcgcctgc tccttcgaca agctgatcgc cgtgttccag
      841 ggcatcatct gcgagagact cgccccagat tcctccacca caactaccac caccaccgac
      901 gacgtgctcg atgtgctcct ccagctgttc aagcagaacg agctgacgat gggcgagatc
      961 aaccacctcc tcgtggacat cttcgacgcc ggcaccgata ccacatcctc cacattcgag
     1021 tgggtgatga ccgagctgat ccgcaatcca gagatgatgg aaaaggccca agaggaaatc
     1081 aagcaggtcc tcggcaagga caagcagatc caagagtccg acatcatcaa cctgccgtac
     1141 ctccaggcga tcatcaaaga gacactccgc ctccatccgc cgaccgtgtt cttgctccca
     1201 agaaaggccg acaccgatgt cgagctgtac ggctacatcg tgccgaagga tgcccagatc
     1261 ctcgtgaacc tctgggccat tggcagggac ccaaacgcct ggcagaacgc cgatattttc
     1321 agcccagagc gcttcatcgg ctgcgagatc gatgttaagg gccgcgattt cggcctcctt
     1381 ccatttggcg ctggccgcag aatttgccca ggcatgaatc tcgccatcag gatgctcacc
     1441 ctcatgctcg ccacactcct ccagttcttc aactggaagc tcgaaggcga catctccccg
     1501 aaggacctcg acatggacga gaagttcggc attgcgctcc aaaagaccaa gccgctcaag
     1561 ctcatcccga ttccgcgcta cggtagcgga gctaccaatt ttagcctcct taagcaggct
     1621 ggtgatgtag aagagaaccc cgggcctatg aagatgatga acggcgagga cgccaacgac
     1681 cagatgatca aagagtcctt cttcatcacc cacggcaacc cgatcctcac cgtcgaggat
     1741 acacatccgc tcaggccgtt cttcgagaca tggcgcgaga agattttctc caagaagccg
     1801 aaggccatcc tcatcatctc cggccactgg gagacagtga agccaaccgt gaacgccgtg
     1861 cacatcaacg acaccatcca cgacttcgac gactacccag ccgccatgta ccagttcaag
     1921 tacccagctc caggcgagcc agagcttgcg agaaaggtgg aagagatcct caagaagtcc
     1981 gggttcgaga cagccgagac agaccaaaag aggggccttg atcacggcgc ctgggttcca
     2041 ctcatgctca tgtatccaga ggcggacatc ccggtgtgcc agctctcagt tcagccacat
     2101 ctcgacggca cctaccacta caatctcggc agagccctcg cgccgctcaa gaatgatggc
     2161 gtgctcatta ttggctccgg cagcgccaca catccactcg atgagacacc gcactacttc
     2221 gatggtgttg ccccttgggc cgctgccttc gattcttggc ttaggaaggc cctcatcaac
     2281 ggccgcttcg aggaagtgaa catctacgag agcaaggccc cgaactggaa gctcgcccat
     2341 ccatttccag agcacttcta cccgctccac gttgtgctcg gcgctgctgg tgaaaagtgg
     2401 aaggccgagc tgatccactc ctcctgggat catggcacac tttgccacgg ctcctacaag
     2461 ttcacctccg ccggatccgg agcaaccaac tttagcctgc tcaagcaagc aggagatgtt
     2521 gaggaaaatc ctggccccat gaccgccatc aagatgaaca ccaacggcga gggcgagaca
     2581 cagcacatcc tcatgatccc gttcatggcg cagggccacc tcaggccatt tctcgaactc
     2641 gccatgttcc tctacaagcg ctcccacgtg atcatcaccc tgctcacaac tccgctcaat
     2701 gccggcttcc tcaggcacct ccttcaccac cattcctact cctccagcgg catcaggatc
     2761 gtcgagctgc cattcaactc caccaaccac ggactcccac cgggcatcga gaacaccgat
     2821 aagctcacac tcccgctcgt ggtgtccctc ttccattcca ccatcagcct cgatccgcac
     2881 ctccgcgatt acatctccag gcatttcagc ccagccaggc caccactctg cgtgatccat
     2941 gatgtgttcc tcggctgggt tgaccaggtg gccaaggatg tgggctctac aggcgtggtg
     3001 ttcacaacag gcggcgctta tggcacatcc gcctacgtgt ccatctggaa cgatctcccg
     3061 caccagaact actccgacga ccaagagttc ccgctgccag gcttcccaga gaaccataag
     3121 ttccgcaggt cccagctcca tcggttcctc agatatgccg acggctccga cgattggtcc
     3181 aagtatttcc agccgcagct ccgccagtcc atgaagtctt ttggctggct ctgcaactcc
     3241 gtggaagaga tcgagacact cggcttctcc atcctccgca actacaccaa gctgccgatc
     3301 tggggcatcg gcccacttat tgcttcccca gtgcagcact cctcctccga caacaattca
     3361 acaggcgccg agttcgtgca gtggctcagc ctcaaagagc cggactccgt cctctacatc
     3421 tccttcggct cccagaacac gatcagcccg acgcagatga tggaactcgc tgctggcctt
     3481 gagtcctccg agaagccatt cctctgggtg atcagagccc cgttcggctt cgacatcaac
     3541 gaagagatgc gcccagagtg gctgccagag ggctttgagg aacgcatgaa ggtgaagaaa
     3601 cagggcaagc tcgtgtacaa gctcggcccg cagcttgaga tcctcaacca tgaatccatc
     3661 ggcggctttc tcacccactg cggatggaac agcatccttg agtctcttcg cgagggcgtt
     3721 ccgatgcttg gatggccact tgctgccgag caggcctaca acctcaagta cctcgaagat
     3781 gagatgggcg tcgcggttga gcttgctaga ggcctcgaag gcgagatctc caaagagaag
     3841 gtcaagcgca tcgtcgagat gatccttgag cgcaacgagg gctccaaagg ctgggagatg
     3901 aagaatcgcg ccgtggaaat gggcaaaaag ctcaaggacg ccgtgaacga ggaaaaagag
     3961 ctgaagggct cctccgtgaa ggcgatcgac gatttcctcg acgccgtcat gcaggccaaa
     4021 cttgagccaa gcctccagtg atagtgagct ttgagaccac gaagtggctc ttcagtggac
     4081 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4141 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4261 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     4321 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
     4381 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     4441 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     4501 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     4561 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     4621 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     4681 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     4741 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     4801 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     4861 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     4921 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     4981 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     5041 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     5101 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     5161 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     5221 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     5281 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5341 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5581 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6181 ccgcagccga acgaccgagc gcagcga
//
