LOCUS       sequence-391097-        2332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-Z-SpCas9-gRNA-repeat-polyT-11
FEATURES             Location/Qualifiers
     source          1..2332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        90..165
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(212..230)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        298..402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             624..1415
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1586..2174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2075..2094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2328..2332,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag ttttagagct agaaatagca agttaaaata
      121 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttcgcttgag
      181 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt
      241 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      301 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      361 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg
      421 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      481 tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
      541 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
      601 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
      661 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
      721 tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta
      781 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
      841 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
      901 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
      961 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1021 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     1081 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     1141 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     1201 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     1261 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     1321 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg
     1381 taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata
     1441 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1501 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1561 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1621 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1681 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1741 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1801 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1861 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1921 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1981 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2041 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2101 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2161 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2221 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2281 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc ga
//
