LOCUS       sequence-391101-        3198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-ZmUbi-11
FEATURES             Location/Qualifiers
     source          1..3198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1078..1096)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1164..1268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1490..2281
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2452..3040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2941..2960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3194..3198,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttgtcatgg gtcgtttaag ctgccgatgt
      121 gcctgcgtcg tctggtgccc tctctccata tggaggttgt caaagtatct gctgttcgtg
      181 tcatgagtcg tgtcagtgtt ggtttaataa tggaccggtt gtgttgtgtg tgcgtactac
      241 ccagaactat gacaaatcat gaataagttt gatgtttgaa attaaagcct gtgctcatta
      301 tgttctgtct ttcagttgtc tcctaatatt tgcctgcagg tactggctat ctaccgtttc
      361 ttacttagga ggtgtttgaa tgcactaaaa ctaatagtta gtggctaaaa ttagttaaaa
      421 catccaaaca ccatagctaa tagttgaact attagctatt tttggaaaat tagttaatag
      481 tgaggtagtt atttgttagc tagctaattc aactaacaat ttttagccaa ctaacaatta
      541 gtttcagtgc attcaaacac ccccttaatg ttaacgtggt tctatctacc gtgtcctaat
      601 atatggttga ttgttcggtt tgttgctatg ctattgggtt ctgattgctg ctagttcttg
      661 ctgaatccag aagttctcgt agtatagctc agattcatat tatttatttg agtgataagt
      721 gatccaggtt attactatgt tagctaggtt ttttttacaa ggataaatta tctgtgatca
      781 taattcttat gaaagcttta tgtttcctgg aggcagtggc atgcaatgca tgacagcaac
      841 ttgatcacac cagctgaggt agatacggta acaaggttct taaatctgtt caccaaatca
      901 ttggagaaca cacatacaca ttcttgccag tcttggttag agaaatttca tgacaaaatg
      961 ccaaagctgt cttgactctt cacttttggc catgagtcgt gacttagttt ggtttaatgg
     1021 accggttctc ctagcttgcg cttgagacca cgaagtggct cttcagtgga cgaaagggcc
     1081 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1141 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1201 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1261 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1321 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
     1381 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1441 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     1501 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1561 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1621 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1681 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1741 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     1801 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     1861 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     1921 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     1981 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2041 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2101 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2161 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2221 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     2281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2581 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3061 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3181 aacgaccgag cgcagcga
//
