LOCUS       sequence-391106-        9167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with 6 FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT 6FLAG
FEATURES             Location/Qualifiers
     source          1..9167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2460..2482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2474..2491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2475..2491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2501..2520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2501..2519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2544..2560
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2545..2561
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2676..2699
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2706..2729
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2736..2759
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2766..2789
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2808..2831
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2838..2861
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        3332..3733
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             3755..3775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4313..4339
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4349..4405
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4442..4462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4478..6199
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(6394..6414)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6394..6412)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6433..6449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6433..6449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6446..6468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6457..6473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6481..6487,6488..6505,6506..6511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6526..6547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6664..6681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6835..7423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6915..6934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7594..8385,8386..8454))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8217..8236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8455..8559)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8596..9099
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9140..9158
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2461 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2521 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2581 cctgcagcta tattaccctg ttatccctac gtacgctgca ggtcgacggt ggcggttctg
     2641 gcggtggcgg atccggtttg aagcttacca tggcagatta caaagacgat gatgacaagt
     2701 caagtgacta taaggatgac gatgacaaga gcagtgatta taaagatgat gacgataaat
     2761 catctgacta caaggacgac gatgataaag ttccagatta cgctgctgat tacaaagacg
     2821 atgatgacaa gtcaagtgac tataaggatg acgatgacaa gagctctaga tgacttaaga
     2881 gttcttcttt gagatatcga ttgaacgagc tcgaattcat cgatctatat taccctgtta
     2941 tccctagcgt aactatgcca aatgacaata agacacccaa taggtccagc actcccaagt
     3001 ttactaaaaa accagtaacc ccgaatgata aaattcctga aagacgggtg tacaatatgg
     3061 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
     3121 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
     3181 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
     3241 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
     3301 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
     3361 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca
     3421 ctaaaggaaa aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat
     3481 gaggggtatc tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct
     3541 aatgagcaac ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt
     3601 gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
     3661 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
     3721 catacaatca actatctcat atacaactag aatgcccaag aagaaacgta aggtgggttc
     3781 cacccttacg agcggtcaac gtgaccacag agacgttgaa aatgagttat ccagtgcagc
     3841 ttttagcagt caaagtttcg gggttccaga tatgtcattc aaaccggggt gctccagtga
     3901 cgtcgcagtc actgatgcgg gtatgcctag ccaagggtta tggaacaacc agacgcaaag
     3961 gatgcgtacc ttcactaagg tccagaaaag gggctctgtt gggaggagta tcgacatcac
     4021 caggtatcgt gattacgatg agctacgtca tgatctggca tgtatgttcg gcatacaggg
     4081 gcagttagaa gacccttatc gtatggattg gaagttagtt tatgtggatc acgagaacga
     4141 tatactgttg gtgggcgacg acccgtggga ggaatttgta ggttgtgtta agtctatcaa
     4201 gattctatct gctgcagagg tgcagcaaat gagcttggac ggggatcttg gtggagttcc
     4261 gccccaaacc caggcgtgca gtgcttcaga cgatgcgaac gcttggagag gctatcccta
     4321 cgacgtgcct gattatgcgg gtagcggggc cactaacttc agcttgttga agcaggctgg
     4381 cgatgtggag gagaatccag gtccggcggc taccgataaa gcggaattaa ttcccgagcc
     4441 tccaaaaaag aagagaaagg tcgaattggg taccgccacg tacttcccgg aggaggtggt
     4501 ggagcacatc ttcagcttcc tgccggcgca gcgcgaccgc aacacggtct cgctcgtctg
     4561 caaggtgtgg tacgagatcg agaggctgag ccgccgcggc gtcttcgtgg gcaactgcta
     4621 cgccgtgcgc gccggccgcg tcgccgcgcg gttccccaac gtgcgggcgc tcacggtgaa
     4681 ggggaagccc cacttcgccg acttcaacct cgtgcccccc gactggggcg gctacgcggg
     4741 gccgtggatc gaggcggccg cgaggggatg ccacggcctg gaggagctca ggatgaagcg
     4801 gatggtggtg tccgacgaga gcctcgagct gctggctcgc tcgttcccgc ggttcagggc
     4861 tcttgttctt atcagctgcg aggggttcag cactgacggg ctagccgccg tcgcgagcca
     4921 ttgcaagctt ctgagggagt tggatttgca ggaaaatgaa gtggaggatc gagggcctag
     4981 gtggctttcc tgcttccctg attcctgcac atcacttgtc tcattgaatt ttgcctgcat
     5041 caaaggggag gttaatgctg gttcactgga gagacttgtt agcaggtccc caaacctgcg
     5101 gagtttgagg ctgaatcgat ctgtatcggt agatacactt gcaaagatac tactgcgtac
     5161 ccctaacttg gaggatttgg ggacagggaa tttgacagat gacttccaaa ctgagtccta
     5221 ctttaagctt accagtgctc tggagaaatg caagatgttg aggagtttgt ctggattctg
     5281 ggatgcttct cctgtttgcc tgtcatttat ctaccccctg tgtgctcaac tgacaggatt
     5341 gaacttgagc tatgcaccca cacttgatgc ttctgacctt acaaaaatga ttagccgctg
     5401 tgtgaagctc caacgccttt gggtactgga ttgtatctcg gacaaaggct tgcaagtggt
     5461 ggcctccagt tgcaaagact tgcaagaact cagggtattt ccatcagatt tctacgtagc
     5521 tggttattct gcagtgacag aggagggact tgttgcagta tccttgggct gtccaaaact
     5581 gaactcacta ctgtacttct gtcaccaaat gactaatgct gcactagtta ctgtcgccaa
     5641 gaactgtcca aatttcacac gattcagact ttgtattctt gagccaggga agcctgatgt
     5701 tgtgacaagc caaccattag atgaaggctt tggagctatt gttcgtgagt gcaagggatt
     5761 acaacgtttg tcaatatctg gtcttctcac agacaaagtt ttcatgtata ttgggaaata
     5821 tgcaaaacaa cttgagatgc tttctatagc atttgctggt gacagtgata agggtatgat
     5881 gcatgttatg aatggatgca agaatttaag gaaactggag ataagagata gcccgtttgg
     5941 tgatgctgca ctcttgggga attttgctag gtacgagaca atgcgatccc tttggatgtc
     6001 atcttgcaat gtcacgttaa aggggtgcca agtccttgcg tcaaagatgc cgatgctcaa
     6061 tgttgaggtc ataaatgagc gggatggtag caatgaaatg gaggaaaacc atggagatct
     6121 gcctaaagtg gagaaattat atgtgtaccg cacaactgct ggggcgaggg atgatgcacc
     6181 aaattttgtt aaaatcctag tcgactccgg ttctgctgct agtggttgaa cgcgtggcgc
     6241 ctggaaaaag attttcaagt gaataaggag ataaaaccgt attgactttt tatcattcta
     6301 caaaagacca gtaacgaagt gtaccctgct atattaccct gttatcccta gcgtaactcg
     6361 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     6421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6481 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7261 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7381 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7441 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7501 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7561 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7621 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7681 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7741 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7801 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7861 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7921 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7981 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8041 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8101 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8161 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8221 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8281 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8341 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8401 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8461 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8521 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8581 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
     8641 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     8701 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
     8761 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
     8821 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     8881 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     8941 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
     9001 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
     9061 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtctaagaaa ccattattat
     9121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
