LOCUS       sequence-391109-        2887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-StUbi-11
FEATURES             Location/Qualifiers
     source          1..2887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(767..785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        853..957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1179..1970
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2141..2729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2630..2649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2883..2887,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttgttgcct gttgttggtt gtcgtgttgt
      121 ctggctgtgt ctgttaccca ttgtggtggt tatgtgtttg cattatggtc ttgaaggacc
      181 atcaatgtgt tttcgctttc tgttcctttc tgtttctcat ttgtgaataa taatggtgtc
      241 tttatgaaca tccagtttct ggtttctttt atgattgcag ttttgagtta tttgtgtttg
      301 cttttgcttc cgtctactac actactttgc aattactata tcactaatgc aggcttgata
      361 tactaaagcc ttcgatccat cttctgtttg atgattcgaa tggtatttat ttaactcata
      421 cccaagtgaa gcataaagtt agaggagagt tcctgttgca ttgcctgttt gtatcatgag
      481 caactcatgt taataaacat aagaaaaacc atgatgcaat ctgtgtaggt gatagacttt
      541 gatgacagac ggactcataa gtaacaagag ataacaaaga ggaaacataa taaacatgta
      601 cgggaagtcc tccaacaatg actttaatca catgtttttg tagattagca attgtacatg
      661 tcaaatgatc ttggattaag gatgtgaatc aaaacatctg aatttggacc tagagtcttg
      721 aggtgatcgc ttgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc
      781 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      961 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     1021 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca
     1081 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     1141 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg
     1201 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     1261 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata
     1321 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     1381 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     1441 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     1501 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     1561 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     1621 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     1681 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     1741 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     1801 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1861 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     1921 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc
     1981 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2041 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2101 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2161 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2221 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2281 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2341 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2401 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2461 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2521 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2581 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2641 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2701 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2761 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2821 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2881 gcagcga
//
