LOCUS       sequence-391111-        2653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-SlEf1a-11
FEATURES             Location/Qualifiers
     source          1..2653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      329..338
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(533..551)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        619..723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             945..1736
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1907..2495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2396..2415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2649..2653,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttactgtgc agtgtgatgc tggtgtttcg
      121 aatgctattt atttacatcg agtgtgctat ctagactctg ttttatttaa ttctgcttta
      181 gtttggtctt tgagttgtgt attaggttgt agttctctga cttgtatctc aagtatccat
      241 tctcagaact gggtacttga taggcggtgg cgagcatgat tttgagtctg ttatcatttg
      301 cttttgttga ggtgccttgg tttgttttcc atggtggctc aggatttttg ttgctttaat
      361 ttgagattta agttttctac tcattacgtt acatttaagt taacatgtta cctttgctaa
      421 agggtggtga tttggttatt aatagctcag ttgtataatg gtgcatcaaa ttctgcctgt
      481 aggatttgtt gcacgcttga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg
      541 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      721 agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      781 gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg
      841 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
      901 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
      961 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     1021 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     1081 gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     1141 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     1201 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     1261 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     1321 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     1381 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     1441 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     1501 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     1561 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     1621 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1681 tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt
     1741 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1801 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2041 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2281 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2581 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2641 ccgagcgcag cga
//
