LOCUS       sequence-391114-        2652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-OsUbi-11
FEATURES             Location/Qualifiers
     source          1..2652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(532..550)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        618..722
                     /gene="bla"
                     /label=AmpR promoter
     CDS             944..1735
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1906..2494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2395..2414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2648..2652,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttgtcctca gccatggagc tgctgctgtt
      121 ctagggttca caagtctgcc tatttgtgtt ccccaatgga gctatggttg tctggtctgg
      181 tccttggtcg tgtcccgttt cattgtgtac tatttacctg taatgtgtat ccttaagtct
      241 ggtttgatgg tgtctgaaac gttttgctgt ggtagagcag catggaagaa ctataatgaa
      301 taagtgatcc ctaatcattg tgtccaaatt ttgcttctgc tatacccttt tgtgctgttt
      361 cttatgtttt gcttaaaaat ttgatctgac aaacaaattt gtctaaatta tgctcttgtt
      421 ctgactgtgt actgtacaca tttgtattgc taccggtttg gcacatagtt ttctctttga
      481 ttgcagatca aacgcttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga
      541 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      721 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
      781 tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc
      841 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
      901 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
      961 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     1021 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     1081 cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     1141 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     1201 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     1261 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     1321 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     1381 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     1441 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     1501 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     1561 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     1621 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     1681 ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc
     1741 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1801 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1861 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1921 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1981 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2041 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2101 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2161 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2221 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2281 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2341 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2401 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2461 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2521 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2581 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2641 cgagcgcagc ga
//
