LOCUS       sequence-391121-        2658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-NtEf1a-11
FEATURES             Location/Qualifiers
     source          1..2658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(538..556)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        624..728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             950..1741
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1912..2500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2401..2420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2654..2658,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttggttgtg cttttgatgg gattctgttt
      121 ggatgaacta gttttgttta tttgcttcag ttttttccaa ttttgttttg gacatttaac
      181 cagtgctgga tatggctcaa gaagctattg tcatggttct ctagcctctt ttgaggatag
      241 tggagtaatt ttgagcagct tttcttcgta ttcctgttcc cttctcatat ttcgtttagc
      301 aacatgtcac ttttggctgt tctcaatctt atttaatagt agggccttcg tgtgtctgtc
      361 atttgttagc agcagcttat ttatcttaat gttgctgata tagtattaag attgtgagct
      421 tattatgatt ctcctaaatt gccataggag aatcataatt gtttttgcat tagattcaac
      481 tttgttggac tgtttttccg cttgagacca cgaagtggct cttcagtgga cgaaagggcc
      541 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      721 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
      781 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
      841 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      901 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
      961 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1021 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1081 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1141 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1201 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     1261 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     1321 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     1381 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     1441 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     1501 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     1561 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     1621 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1681 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     1741 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1801 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1861 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1921 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1981 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2041 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2101 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2161 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2221 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2281 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2341 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2401 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2461 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2521 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2581 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2641 aacgaccgag cgcagcga
//
