LOCUS       sequence-391128-        3297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-NbAct3-11
FEATURES             Location/Qualifiers
     source          1..3297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1177..1195)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1263..1367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1589..2380
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2551..3139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3040..3059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3293..3297,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttatacagc attcccagaa agagaaacag
      121 aagaaatata caaactttca ttttgagagc agcacctcgt ctattgattg cagataatat
      181 gcttctcatt tgtatttcct tttgattatt tttgtttcta tccctttgtt tgagtcaatc
      241 tcaaatattc ggtcattgtt ggtatgaaaa atcaagcagt tcatgttaag agtcaattta
      301 aaattaatat ttttatatag agttgtatgt gaaatgatgt tgtgatttgg tatatatgga
      361 taaagagctt gtcagttcat tttggtctga tttttttggt atccaaataa gaaacacaaa
      421 agggatatgt ccctctacta tcaaatatta gttataagta ttcatgttat actattcgat
      481 attttctacc ccaatcgtta cctatttaaa agtatttacc cctccatcta tcaaacccct
      541 ggacccagct ttcctattac atgtggcttc atcttaagcc cccaaacctt tttcttattt
      601 ttgattttta aaggctcatc ttaaaattta ttactcaaat taatacctct taataaccca
      661 cctcaaggac ccagtaatta aatatccaat tagctccagt aattggggtt catattagct
      721 ccagtcttaa attttaaagg cgatgatcgt attcctccac ttggttcatt tatactcaaa
      781 gaatactcaa tgtctttagt gtttagataa ctttttgtaa atcatataga ttgttttaac
      841 aaaaaacaat tcaatagtag attttcacat gaaagttaca taaaaattct ttaaaattac
      901 tttctcaaaa aattgttcca aacatattat cccacaatta aactcaatct gtttttcgaa
      961 acctaaatca aaaccaatcc aactacctta tataatatat aatcaataca ttgtaaagaa
     1021 ctgcatgttc ttttaaattt tgggggcaaa gttattccgt acgttcacac atgtactaat
     1081 aggaggtaat aaatgatatg tgaaacaatc gaggtgtaaa caagctagca tgaattccgc
     1141 ttgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat
     1201 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     1261 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1321 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca
     1381 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     1441 cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     1501 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     1561 cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac
     1621 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     1681 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct
     1741 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     1801 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     1861 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     1921 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     1981 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     2041 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     2101 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     2161 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     2221 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     2281 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     2341 ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc
     2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcga
//
