LOCUS       sequence-391144-        5355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-ZmBbm-P2A-ZmWus2-9
FEATURES             Location/Qualifiers
     source          1..5355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1033..1044
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2230..2286
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(3235..3253)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3321..3425
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3647..4438
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4609..5197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5098..5117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(5351..5355,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggccactg tgaacaactg gctcgctttc
      121 tccctctccc cgcaggagct gccgccctcc cagacgacgg actccacact catctcggcc
      181 gccaccgccg accatgtctc cggcgatgtc tgcttcaaca tcccccaaga ttggagcatg
      241 aggggatcag agctttcggc gctcgtcgcg gagccgaagc tggaggactt cctcggcggc
      301 atctccttct ccgagcagca tcacaaggcc aactgcaaca tgatacccag cactagcagc
      361 acagtttgct acgccagctc aggtgctagc accggctacc atcaccagct gtaccaccag
      421 cccaccagct cagcgctcca cttcgcggac tccgtaatgg tggcttcctc ggccggtgtc
      481 cacgacggcg gtgccatgct cagcgcggcc gccgctaacg gtgtcgctgg cgctgccagt
      541 gccaacggcg gcggcatcgg gctgtccatg attaagaact ggctgcggag ccaaccggcg
      601 cccatgcagc ctagggtggc ggcggctgag ggcgcgcagg ggctctcttt gtccatgaac
      661 atggcgggga cgacccaagg cgctgctggc atgccacttc tcgctggaga gcgcgcacgg
      721 gcgcccgaga gtgtatcgac gtcagcacag ggtggagccg tcgtcgtcac ggcgccgaag
      781 gaggatagcg gtggcagcgg tgttgccggc gctctagtag ccgtgagcac ggacacgggt
      841 ggcagcggcg gcgcgtcggc tgacaacacg gcaagaaaga cggtggacac gttcgggcag
      901 cgcacgtcga tttaccgtgg cgtgacaagg catagatgga ctgggagata tgaggcacat
      961 ctttgggata acagttgcag aagggaaggg caaactcgta agggtcgtca agtctattta
     1021 ggtggctatg ataaagagga gaaagctgct agggcttatg atcttgctgc tctgaagtac
     1081 tggggtgcca caacaacaac aaattttcca gtgagtaact acgaaaagga gctggaggac
     1141 atgaagcaca tgacaaggca ggagtttgta gcctctctga gaaggaagtc cagtggtttc
     1201 tccagaggtg catccattta caggggagtg actaggcatc accaacatgg aagatggcaa
     1261 gcacggattg gacgagttgc agggaacaag gatctttact tgggcacctt cagcacccag
     1321 gaggaggcag cggaggcgta cgacatcgcg gcgatcaagt tccgcggcct caacgccgtc
     1381 accaacttcg acatgagccg ctacgacgtc aagagcatcc tggacagcag cgccctcccc
     1441 atcggcagcg ccgccaagcg tctgaaggag gccgaggccg cagcgtccgc gcagcaccac
     1501 cacgccggcg tggtgagcta cgacgtcggc cgcatcgcct cgcagctcgg cgacggcgga
     1561 gccctagcgg cggcgtacgg cgcgcactac cacggcgccg cctggccgac catcgcgttc
     1621 cagccgggcg ccgccagcac aggcctgtac cacccgtacg cgcagcagcc aatgcgcggc
     1681 ggcgggtggt gcaagcagga gcaggaccac gcggtgatcg cggccgcgca cagcctccag
     1741 gacctccacc acctgaacct gggcgcggcc ggcgcgcacg actttttctc ggcagggcag
     1801 caggccgccg ccgctgcgat gcacggcctg ggtagcatcg acagtgcgtc gctggagcac
     1861 agcaccggct ccaactccgt cgtctacaac ggcggggtcg gcgacagcaa cggcgccagc
     1921 gccgtcggcg gcagtggcgg tggctacatg atgccgatga gcgctgccgg agcaaccact
     1981 acatcggcaa tggtgagcca cgagcaggtc catgcacggg cctacgacga agccaagcag
     2041 gctgctcaga tggggtacga gagctacctg gtgaacgcgg agaacaatgg tggcggaagg
     2101 atgtctgcat gggggactgt cgtgtctgca gccgcggcgg cagcagcaag cagcaacgac
     2161 aacatggccg ccgacgtcgg gcatggcggc gcgcagctgt tcagtgtctg gaacgacact
     2221 ggctccggcg ccaccaactt ctccctcctg aagcaggccg gcgacgtcga ggagaacccc
     2281 ggcccgatgg cggccaatgc gggcggcggt ggagcgggag gaggcagcgg cagcggcagc
     2341 gtggctgcgc cggcggtgtg ccgccccagc ggctcgcggt ggacgccgac gccggagcag
     2401 atcaggatgc tgaaggagct gtactacggc tgcggcatcc ggtcgcccag ctcggagcag
     2461 atccagcgca tcaccgccat gctgcggcag cacggcaaga tcgagggcaa gaacgttttc
     2521 tactggttcc agaaccacaa ggcccgcgag cgccagaagc gccgcctcac cagcctcgac
     2581 gtgaacgtgc ccgccgccgg cgcggccgac gccaccacca gccaactcgg cgtcctctcg
     2641 ctgtcgtcgc cgccgccttc aggcgcggcg cctccctcgc ccaccctcgg cttctacgcc
     2701 gccggcaatg gcggcggatc ggctgtgctg ctggacacga gttccgactg gggcagcagc
     2761 ggcgctgcga tggccaccga gacatgcttc ctccaggact acatgggcgt gacggacacg
     2821 ggcagctcgt cgcagtggcc acgcttctcg tcgtcggaca cgataatggc ggcggccgcg
     2881 gcgcgggcgg cgacgacgcg ggcgcccgag actctccctc tcttcccgac ctgcggcgac
     2941 gacggcggca gcggtagcag cagctacttg ccgttctggg gtgccgcgtc cacaactgcc
     3001 ggcgccactt cttccgttgc gatccagcag caacaccagc tgcaggagca gtacagcttt
     3061 tacagcaaca gcaacagcac ccagctggcc ggcaccggca accaagacgt atcggcaaca
     3121 gcagcagcag ccgccgccct ggagctgagc ctcagctcat ggtgctcccc ttaccctgct
     3181 gcagggagta tgtgagcttt gagaccacga agtggctctt cagtggacga aagggcctcg
     3241 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     3301 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3361 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3421 agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     3481 cagtggcggt tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg
     3541 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     3601 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt
     3661 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     3721 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     3781 cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     3841 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     3901 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     3961 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     4021 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     4081 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     4141 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     4201 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     4261 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     4321 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     4381 gttggaagaa tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact
     4441 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4501 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4561 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4621 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4681 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4741 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4801 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4861 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4921 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4981 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5041 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5101 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5161 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5221 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5281 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5341 gaccgagcgc agcga
//
