LOCUS       sequence-391145-        3159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-ZmWus2-9
FEATURES             Location/Qualifiers
     source          1..3159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1039..1057)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1125..1229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1451..2242
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2413..3001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2902..2921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3155..3159,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggcggcca atgcgggcgg cggtggagcg
      121 ggaggaggca gcggcagcgg cagcgtggct gcgccggcgg tgtgccgccc cagcggctcg
      181 cggtggacgc cgacgccgga gcagatcagg atgctgaagg agctgtacta cggctgcggc
      241 atccggtcgc ccagctcgga gcagatccag cgcatcaccg ccatgctgcg gcagcacggc
      301 aagatcgagg gcaagaacgt cttctactgg ttccagaacc acaaggcccg cgagcgccag
      361 aagcgccgcc tcaccagcct cgacgtgaac gtgcccgccg ccggcgcggc cgacgccacc
      421 accagccaac tcggcgtcct ctcgctgtcg tcgccgccgc cttcaggcgc ggcgcctccc
      481 tcgcccaccc tcggcttcta cgccgccggc aatggcggcg gatcggctgt gctgctggac
      541 acgagttccg actggggcag cagcggcgct gcgatggcca ccgagacatg cttcctccag
      601 gactacatgg gcgtgacgga cacgggcagc tcgtcgcagt ggccacgctt ctcgtcgtcg
      661 gacacgataa tggcggcggc cgcggcgcgg gcggcgacga cgcgggcgcc cgagactctc
      721 cctctcttcc cgacctgcgg cgacgacggc ggcagcggta gcagcagcta cttgccgttc
      781 tggggtgccg cgtccacaac tgccggcgcc acttcttccg ttgcgatcca gcagcaacac
      841 cagctgcagg agcagtacag cttttacagc aacagcaaca gcacccagct ggccggcacc
      901 ggcaaccaag acgtatcggc aacagcagca gcagccgccg ccctggagct gagcctcagc
      961 tcatggtgct ccccttaccc tgctgcaggg agtatgtgag ctttgagacc acgaagtggc
     1021 tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1081 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1141 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1201 tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga
     1261 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1321 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     1381 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     1441 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     1501 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     1561 agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag
     1621 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     1681 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     1741 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     1801 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     1861 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     1921 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     1981 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2041 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2101 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     2161 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat
     2221 caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga
     2281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2341 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2401 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2461 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2521 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2581 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2641 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2701 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2761 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2821 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2881 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2941 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3001 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3061 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3121 tgataccgct cgccgcagcc gaacgaccga gcgcagcga
//
