LOCUS       sequence-391147-        4383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-ZmBbm-9
FEATURES             Location/Qualifiers
     source          1..4383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1033..1044
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(2263..2281)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2349..2453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2675..3466
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3637..4225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4126..4145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4379..4383,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggccactg tgaacaactg gctcgctttc
      121 tccctctccc cgcaggagct gccgccctcc cagacgacgg actccacact catctcggcc
      181 gccaccgccg accatgtctc cggcgatgtc tgcttcaaca tcccccaaga ttggagcatg
      241 aggggatcag agctttcggc gctcgtcgcg gagccgaagc tggaggactt cctcggcggc
      301 atctccttct ccgagcagca tcacaaggcc aactgcaaca tgatacccag cactagcagc
      361 acagtttgct acgccagctc aggtgctagc accggctacc atcaccagct gtaccaccag
      421 cccaccagct cagcgctcca cttcgcggac tccgtaatgg tggcttcctc ggccggtgtc
      481 cacgacggcg gtgccatgct cagcgcggcc gccgctaacg gtgtcgctgg cgctgccagt
      541 gccaacggcg gcggcatcgg gctgtccatg attaagaact ggctgcggag ccaaccggcg
      601 cccatgcagc ctagggtggc ggcggctgag ggcgcgcagg ggctctcttt gtccatgaac
      661 atggcgggga cgacccaagg cgctgctggc atgccacttc tcgctggaga gcgcgcacgg
      721 gcgcccgaga gtgtatcgac gtcagcacag ggtggagccg tcgtcgtcac ggcgccgaag
      781 gaggatagcg gtggcagcgg tgttgccggc gctctagtag ccgtgagcac ggacacgggt
      841 ggcagcggcg gcgcgtcggc tgacaacacg gcaagaaaga cggtggacac gttcgggcag
      901 cgcacgtcga tttaccgtgg cgtgacaagg catagatgga ctgggagata tgaggcacat
      961 ctttgggata acagttgcag aagggaaggg caaactcgta agggtcgtca agtctattta
     1021 ggtggctatg ataaagagga gaaagctgct agggcttatg atcttgctgc tctgaagtac
     1081 tggggtgcca caacaacaac aaattttcca gtgagtaact acgaaaagga gctggaggac
     1141 atgaagcaca tgacaaggca ggagtttgta gcctctctga gaaggaagtc cagtggtttc
     1201 tccagaggtg catccattta caggggagtg actaggcatc accaacatgg aagatggcaa
     1261 gcacggattg gacgagttgc agggaacaag gatctttact tgggcacctt cagcacccag
     1321 gaggaggcag cggaggcgta cgacatcgcg gcgatcaagt tccgcggcct caacgccgtc
     1381 accaacttcg acatgagccg ctacgacgtc aagagcatcc tggacagcag cgccctcccc
     1441 atcggcagcg ccgccaagcg tctgaaggag gccgaggccg cagcgtccgc gcagcaccac
     1501 cacgccggcg tggtgagcta cgacgtcggc cgcatcgcct cgcagctcgg cgacggcgga
     1561 gccctagcgg cggcgtacgg cgcgcactac cacggcgccg cctggccgac catcgcgttc
     1621 cagccgggcg ccgccagcac aggcctgtac cacccgtacg cgcagcagcc aatgcgcggc
     1681 ggcgggtggt gcaagcagga gcaggaccac gcggtgatcg cggccgcgca cagcctccag
     1741 gacctccacc acctgaacct gggcgcggcc ggcgcgcacg actttttctc ggcagggcag
     1801 caggccgccg ccgctgcgat gcacggcctg ggtagcatcg acagtgcgtc gctggagcac
     1861 agcaccggct ccaactccgt cgtctacaac ggcggggtcg gcgacagcaa cggcgccagc
     1921 gccgtcggcg gcagtggcgg tggctacatg atgccgatga gcgctgccgg agcaaccact
     1981 acatcggcaa tggtgagcca cgagcaggtc catgcacggg cctacgacga agccaagcag
     2041 gctgctcaga tggggtacga gagctacctg gtgaacgcgg agaacaatgg tggcggaagg
     2101 atgtctgcat gggggactgt cgtgtctgca gccgcggcgg cagcagcaag cagcaacgac
     2161 aacatggccg ccgacgtcgg gcatggcggc gcgcagctgt tcagtgtctg gaacgacact
     2221 taagctttga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
     2281 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2341 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2401 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
     2461 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2521 tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca
     2581 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     2641 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     2701 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2761 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga
     2821 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     2881 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     2941 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     3001 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     3061 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3121 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3181 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3241 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3301 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3361 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3421 tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt
     3481 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3541 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3601 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3661 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3721 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3781 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3841 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3901 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3961 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4021 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4081 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4141 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4201 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4261 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4321 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4381 cga
//
