LOCUS       sequence-391148-        3927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-AtPLT5-9
FEATURES             Location/Qualifiers
     source          1..3927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1807..1825)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1893..1997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2219..3010
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3181..3769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3670..3689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3923..3927,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgaagaata ataataataa gtcatcttca
      121 tccagttcct acgattcttc actgtctcca agcagttcat cctcatctca ccagaactgg
      181 ttaagtttct ccctgagcaa taacaataac aattttaatt cctctagtaa tccaaacctc
      241 actagttcca catcagacca tcaccatcct caccctagtc atctgtctct gttccaagca
      301 ttctccacct ctcctgtaga acgtcaggac ggatcacccg gagtaagccc cagcgatgct
      361 acagcagtac tttcagttta ccctggcggc ccaaaattag aaaacttcct cgggggtggg
      421 gcaagcacta ctactacaag gccaatgcaa caagtccaat cccttggtgg agtcgtcttt
      481 tcctcagacc tccaaccccc cctacatcct ccttctgcag ccgagatcta tgattctgag
      541 ctgaaaagta tagcagcctc cttccttggt aactacagcg gtggacatag ctccgaagtt
      601 agtagtgttc acaagcaaca accaaaccca cttgccgtca gcgaagctag ccctacccca
      661 aaaaaaaacg tcgaaagttt tggtcagcga acaagtattt acaggggagt gaccagacat
      721 aggtggaccg ggaggtacga ggctcatcta tgggacaaca gttgtagaag agaaggacaa
      781 tctagaaagg gacgacaggt gtacttaggc ggctacgata aggaagataa ggccgcacgt
      841 gcatatgatc tggcagccct caagtactgg ggacctacca ccacaacaaa tttccccatc
      901 tcaaactacg aaagcgaact agaagagatg aagcatatga cacgtcagga gtttgtagct
      961 tctctaagaa gaaagagctc aggtttttcc cgaggcgcta gcatgtaccg tggagttact
     1021 cgacatcatc agcacggaag gtggcaagca aggattggac gtgtagcagg caacaaagac
     1081 ctttacttgg gcaccttctc tacccaagag gaagccgccg aagcttacga cattgcagcc
     1141 atcaagtttc gtggactgaa cgcagtgacc aactttgaca tttcaaggta tgatgtcaaa
     1201 agcatagctt catgtaactt gcccgtgggc ggactcatgc caaaacctag tccagcaact
     1261 gcagcagcag ataaaactgt tgaccttagc ccctctgatt caccctccct caccactcca
     1321 tccttgactt tcaacgtggc cactcccgtc aacgatcacg gcggtacatt ttaccatact
     1381 gggatcccaa ttaaacccga tcccgccgac cactactgga gcaatatctt tggcttccag
     1441 gctaacccca aagctgaaat gaggccctta gcaaacttcg gctctgatct ccacaatcca
     1501 tctcccggtt atgcaattat gcccgtgatg caggagggag aaaacaactt cgggggatcc
     1561 ttcgtcggat cagatggcta caacaaccat agcgcagcat caaaccctgt atccgccata
     1621 ccattaagca gcacaaccac aatgagcaat ggtaatgagg ggtacggagg caacataaat
     1681 tggatcaaca ataatatctc ttccagttac cagactgcca aaagtaatct gtcagtctta
     1741 catacaccag tgtttggcct ggagtaagct ttgagaccac gaagtggctc ttcagtggac
     1801 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1861 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1921 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1981 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     2041 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
     2101 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2161 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     2221 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2281 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2341 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2401 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2461 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2521 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2581 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2641 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     2701 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     2761 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     2821 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     2881 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     2941 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     3001 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3061 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3121 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3181 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3301 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3421 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3541 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3841 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3901 ccgcagccga acgaccgagc gcagcga
//
