LOCUS       sequence-391151-        4531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Honey bee gut symbiont broad-host range vector with constitutive 
            GFP, ampR and RK2 replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Chhun A, Moriano-Gutierrez S, Zoppi F, Cabirol A, Engel P, Schaerli 
            Y
  TITLE     An engineered bacterial symbiont allows noninvasive biosensing of 
            the honey bee gut environment.
  JOURNAL   PLoS Biol. 2024 Mar 5;22(3):e3002523. doi: 
            10.1371/journal.pbio.3002523. eCollection 2024 Mar.
  PUBMED    38442124
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2024-03-5"; volume: "22(3):e3002523. doi"; pages: " 
            10.1371/journal.pbio.3002523. eCollection 2024 Mar"
COMMENT     Sequence Label: pAC26
FEATURES             Location/Qualifiers
     source          1..4531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1366..1897
                     /label=oriV
                     /note="incP origin of replication"
     CDS             2290..3438
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIVSRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     oriT            3499..3607
                     /note="incP origin of transfer"
     promoter        3707..3810
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3811..3879,3880..4531,1..140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4029..4048)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
       61 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
      181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatcgcggcc
      241 gctcgccact gacgagcaga tttccagctg acaaatgctc tttccctaaa ctccccccat
      301 aaaaaaaccc gccgaagcgg gtttttacgt tatttgcgga ttaacgatta cggatttatt
      361 tgtatagttc atccatgccg tgtgtaatcc cagcagccgt gacaaactca agaaggacca
      421 tgtggtcgcg cttttcgttg ggatctttcg aaagggcaga ttgagtggac aggtaatggt
      481 tgtctggtaa cagcacaggc ccatcgccaa ttggggtgtt ttgttggtaa tggtcggcta
      541 gttgaacgct tccatcctca atgttgtgtc tgatcttgaa gttaaccttg ataccgttct
      601 tttgcttgtc ggccataatg tacacattat gggagttata gttatattcc agtttgtggc
      661 ctaaaatatt tccatcttcc ttaaagtcaa taccctttaa ctcgattcta ttaacaaggg
      721 tatcaccctc gaacttcact tcggcgcgtg tcttatagtt gccgtcatcc ttaaaaaaga
      781 tggttctctc ctgtacatag ccttcgggca ttgcactctt gaaaaagtcg tgctgtttca
      841 tgtgatctgg gtagcgcgca aagcactgaa ccccataccc gaacgtagtg accagtgttg
      901 gccatggaac aggtagttta cccgtggtgc aaataaattt aagggtcagc ttgccatatg
      961 ttgcgtcgcc ttcaccctct ccactgacag agaacttgtg cccattaaca tcaccgtcta
     1021 attccacgag gattgggaca actcctgtga aaagctcttc tcctttactc atatgtaata
     1081 tctcctctgt ttctgtataa cagtactatg tgattatacc agccccctca ctacatgtca
     1141 agaataaact gccaaagcgt tgtattgcga cgaattgcca cgttgtcgca gtgtctgtac
     1201 tgtgggttaa tgtctgtgat cgccgtcgtg agttcgtgtc atccgcgcat gattcagatg
     1261 cgcgccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg ggggatcagg
     1321 accgctgccg gagcgcaacc cactcactac agcagagcca tgtagggccg ccggcgttgt
     1381 ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga
     1441 ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg
     1501 acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca
     1561 cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg
     1621 actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga
     1681 ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag
     1741 ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata
     1801 tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag
     1861 gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc catcccccca
     1921 ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcca cgtagaaagc
     1981 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
     2041 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
     2101 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
     2161 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
     2221 gcgcagggga tcaagatcga cggatcgatc cggggaatta attccggggc aatcccgcaa
     2281 ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact gatcgacgcg
     2341 gggttttccg ccgaggatgc cgaaaccatc gtaagccgca ccgtcatgcg tgcgccccgc
     2401 gaaaccttcc agtccgtcgg ctcgatagtc cagcaagcta cggccaagat cgagcgcgac
     2461 agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt
     2521 cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac gcgaggaact
     2581 atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt cagcgaagcc
     2641 aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca gctttccttg
     2701 ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac ggcccgctct
     2761 gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt
     2821 ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg ggccgacgat
     2881 gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat cggcgagccg
     2941 atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa tggccggtat
     3001 tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc
     3061 gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt
     3121 ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct
     3181 ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc gacggcccga
     3241 cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc
     3301 cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa
     3361 gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgatgacctg
     3421 gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagccacc
     3481 tgcatcgcgg ccggcctacg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     3541 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
     3601 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcacgt
     3661 taagggattt tggtcatgac aggtggcact tttcggggaa atgtgtgcgg aacccctatt
     3721 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3781 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3841 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3901 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3961 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4021 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4081 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4141 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4201 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4261 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4321 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4381 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4441 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4501 gataaatctg gagccggtga gcgtgggtcc c
//
