LOCUS       sequence-391154-       16408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEL143; transgenic construct to express cell-specific Cas9 in 
            oligodendrocytes; ubiquitous control sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16408)
  AUTHORS   Lysko DE, Talbot WS
  TITLE     Unmyelinated sensory neurons use Neuregulin signals to promote 
            myelination of interneurons in the CNS.
  JOURNAL   Cell Rep. 2022 Nov 15;41(7):111669. doi: 
            10.1016/j.celrep.2022.111669.
  PUBMED    36384112
REFERENCE   2  (bases 1 to 16408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-11-15"; volume: "41(7):111669. doi"; pages: " 
            10.1016/j.celrep.2022.111669"
COMMENT     Sequence Label: pTol2_claudink:Cas9green; control_gRNA
FEATURES             Location/Qualifiers
     source          1..16408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(122..190,191..982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(340..359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1153..1741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1642..1661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1895..1912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2029..2050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2065..2070,2071..2088,2089..2095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2103..2119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2108..2130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2127..2143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2164..2182
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2219..2235
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2219..2235
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(2269..2280,2281..2468)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     CDS             join(3690..3692,3693..3695,3696..4409)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3690..3692,3693..3695,3696..4406)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4711..4845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_RNA        5244..5319
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     5332..5348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5356..5376
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5800..5824
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5853..5871
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(join(5908..6621,6622..6624,6625..6627))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5911..6621,6622..6624,6625..6627))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(6634..6687)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6703..6723)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6739..10839)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes MGAS15252 Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
                     ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6818..6826
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(10846..10866)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      10875..10884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10920..10938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10948..10964)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(10966..10990)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(15185..15205)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(15225..15241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(15614..15776,15777..15788)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     16241..16262
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
       61 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      121 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      181 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      241 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      301 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      361 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      421 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      481 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      541 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      601 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      661 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      721 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      781 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      841 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      901 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
      961 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1021 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1081 gaccaaaatg ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1201 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1261 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1321 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1381 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1441 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1501 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1561 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1621 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1681 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1741 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1801 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1861 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1921 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     1981 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2041 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2101 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     2161 cgcaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc
     2221 tagaactagt ggatctgctg ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa
     2281 gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg atttttactt
     2341 tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt tttttagaaa
     2401 aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt attttttgga
     2461 gatcacttca ttctattttc ccttgctatt accaaaccaa ttgaattgcg ctgatgccca
     2521 gtttaattta aatgttattt attctgccta tgaaaatcgt tttcacatta tatgaaattg
     2581 gtcagacatg ttcattggtc ctttggaagt gacgtcatgt cacatctatt accacaatgc
     2641 acagcacctt gacctggaaa ttagggaaat tataacagtc aatcagtgga agaaaatgga
     2701 ggaagtatgt gattcatcag cagctgcgag cagcacagtc caaaatcagc cacaggatca
     2761 agagcacccg tggccgtatc ttcgcagatc taaagcttaa atcagttgtg ttaaataaga
     2821 gacattcaaa ataaatgtaa atgagctctc caaatcagca gacttaacat tctttaaaat
     2881 gattgattca atagtgataa aaatcaggca tagccagttg taactttaga taaattacag
     2941 aaaatgtcaa atacagagaa ccgattcttt tttatgatac atccaagcac acatttaaca
     3001 caatccaggc aaaccccgaa tttcacagtc acaagcactg tttgtacaag agctttgcct
     3061 aaggacacac agtctctata agtccaggtc gttggtttca ctcttatttt aaacatgtga
     3121 catttttcct gccatcctgt cttaggctgc tgtttgcttc attccatgtc acattaaatt
     3181 cctcagtagc accttttaca cacacagcca atcttttcca gaaaattcaa ttgctttgaa
     3241 gagataatgt gtgaacaaat ccatttagaa aaggaaaatt aagaatttgt aaaatcatct
     3301 gtaaattgtt ggcattcttc tgtatatgaa catcacatca tttacaggta aaggtctggt
     3361 cattaattat atgacaattt actggtatta ttttgtgaaa ggggctattt tcaatgcgtt
     3421 catccatcct tttcatccct caaatctctc attcacgtcc ccctccccat ctgcacactt
     3481 tatctcattt ccaccctgct ggaatctgag cacttgtgca gttatcaggg ctcctgtatt
     3541 taggaggctc tgggtgtcca tgtaggggac gaacagaaac actgcagacc tttatagaag
     3601 aacaattgat aagagtcctc atacataaag actccattag taagccagtg acccaggagc
     3661 ccagaccaac agcaaagcag acagtgacca tggtgagcaa gggcgaagag ctgttcaccg
     3721 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3781 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3841 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3901 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3961 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4021 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4081 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4141 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     4201 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4261 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4321 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4381 ctctcggcat ggacgagctg tacaagtaaa gcggcccctc tcgagcctct agaactatag
     4441 tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4501 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4561 tgtaaccatt ataagctgca ataaacaaga gatctgatct agaggatcat aatcagccat
     4621 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     4681 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     4741 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4801 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcatcat cgatgcgtct
     4861 tttgttctgg tcatcaagga ggggggaatg ttctgcgcat gcctgtgggg gagggagaag
     4921 gacacgtcac tgaaaacgtc cctgcatcac accgagacac ccaatcactc aagccgagac
     4981 cagataattt tgcatatgct ttaaagtttg aaaaatacca cggtaaaccc tcacacaaac
     5041 tctggatttg agatctttca ggttttatca gtttgcaggt ttatgtcacc atgatatagg
     5101 gtcagacttg atctaaggga gctgaataag tggtttagtc actcaccacc tcccaaaaac
     5161 atacccagaa gtccctggta tatatagctc tccctccagc tcttggttcg gctagcactc
     5221 ctcagtgacg aggagtcagt agcgttttag agctagaaat agcaagttaa aataaggcta
     5281 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttggtac cgtaaaacga
     5341 cggccagtga attatcaact atgtataata aagttgctgg catacactca cttccactaa
     5401 taaaatgaga atattttcaa tgcagaacat ctgtatggag cactttagat tcacacagtt
     5461 cacctgatca atcaaatata aatatgtcaa gatcaaaaac aatgtgcact caccaagtca
     5521 atgcacagac aatatcagtg tgtggtgtgt tatttcacag aaactaactg cacatctaaa
     5581 aataataaac ctaccgtgag aaggagagta caaagtgaac gtggcataca cagagtgagc
     5641 cgatccgagc agcaaagcca tgtagcaaag acaaagaaaa acatcaggca ggaaatttgg
     5701 gcaacatttt atttataggc tttatctttt cagaacatca aatctcaaca gtctccacag
     5761 gttttgacat gttcagttaa cggtggctga gacttaatag ccactttgta caagaaagct
     5821 gggtgctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     5881 ctggagctcc accgcggtgg cggccgctta cttgtacagc tcgtccatgc cgagagtgat
     5941 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
     6001 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     6061 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     6121 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
     6181 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     6241 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     6301 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     6361 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
     6421 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     6481 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     6541 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     6601 gaacagctct tcgcccttgc tcaccattct agaagggccg ggattctctt ccacgtcacc
     6661 gcatgtcagc agactgcctc tgccctctcc gccaccagac ctcacctttc tcttcttctt
     6721 aggggatccc accggtgagt cgccacccag ctgagacagg tcaatccttg tttcgtacag
     6781 gccggtaata ctctgatgga tcagtgtagc gtccagcacc tccttagtgg aggtgtacct
     6841 ctttctatcg attgtagtgt cgaagtactt gaaagctgca ggtgcgccca ggtttgtcag
     6901 agtgaacaga tgaatgatat tttcggcctg ctccctgatg ggcttatctc tgtgtttgtt
     6961 gtaagcagac aggaccttat ccagatttgc gtcggccagg atcactcttt tagagaactc
     7021 gctaatctgt tcaatgatct cgtccagata atgcttgtgc tgctccacaa acagctgttt
     7081 ctgttcgtta tcctctggag atcctttcag cttctcgtag tggcttgcca gatacaggaa
     7141 gttcacgtac ttagaaggca gggccagttc attgcctttc tgcagctctc cagcgcttgc
     7201 cagcatgcgt ttcctgccgt tttccagctc aaacagactg tacttgggca gtttaatgat
     7261 caggtctttc ttcacttcct tataaccttt ggcctccaga aaatcgattg ggttcttctc
     7321 gaaagagctt ctctccataa ttgtgatacc cagcagctcc ttgacagatt tcagtttctt
     7381 gcttttgccc ttctccacct tagcgaccac caggacggag tatgccacag tgggagagtc
     7441 aaagcctcca tacttcttag ggtcccagtc tttctttcta gcgatcagct tatcagagtt
     7501 gcgttttggc agaatacttt cttttgagaa accgcctgtc tgcacctcag ttttcttgac
     7561 gatattcact tgaggcatgc tcaggacctt cctcacggtt gcaaaatctc tgcctttgtc
     7621 ccacacgatt tcgcctgtct ctccgtttgt ctcaatcagg ggccttttgc ggatttctcc
     7681 attggccagg gtgatctctg tcttaaagaa attcatgatg tttgagtaaa agaaatactt
     7741 ggcagtagct tttccaattt cctgctcgga cttggcgatc atttttctga catcgtacac
     7801 cttatagtct ccgtacacga attcgctctc cagttttggg tatttcttga tcagtgcggt
     7861 accgaccacg gcgttcaggt atgcgtcatg ggcatggtga taattgttaa tctcgcggac
     7921 cttataaaac tggaaatctt tcctaaagtc gctgaccagt ttactcttca gggtaatgac
     7981 cttcacttct ctgatcagtt tgtcattctc atcgtacttt gtgttcatgc gactatccag
     8041 aatctgtgcg acgtgtttag tgatctggcg tgtttccacc agctgcctct tgatgaatcc
     8101 agctttgtcc agttctgaca gtccaccgcg ctctgctttt gtcaggttat caaacttcct
     8161 ctgagtgatc agtttggcat tcagcagctg tctccagtag ttcttcattt tcttgaccac
     8221 ttcctcacta gggacattat ctgattttcc tctgtttttg tcggacctgg tcagcacctt
     8281 gttgtcaatg ctgtcatctt tgatgaaact ctgggggacg atatgatcca cgtcgtaatc
     8341 agacagcctg ttaatatcca gctcctggtc cacgtacata tctcttccat tctgcagata
     8401 gtacagatac agcttctcat tctgcagctg tgtgttttcc actgggtgct ctttcaggat
     8461 ctggctaccc agctccttaa tgccttcctc gattcttttc atcctctctc tactgttctt
     8521 ctgacccttc tgggttgtct gattttcgcg agccatctca atcacgatgt tttcgggctt
     8581 atggcccatg actttcacca gctcatcgac gattttcact gtctgcagaa tgcctttctt
     8641 gatggctggg cttccagcca ggtttgcaat ctgctcatgc agggagtggc cctgtccaga
     8701 cacctgagcc ttctggatgt cctctttgaa ggtcagactg tcatcgtgaa tcagctgcat
     8761 aaaattgcgg ttggcgaagc catcactttt cagaaagtcc aggattgtct ttcctgactg
     8821 tttatcccta atgccgttga tcagcttcct gctcagtcta ccccagccag tgtagcgcct
     8881 tctcttcagc tgtttcatca ccttgtcatc gaacagatga gcataggttt tcagtctttc
     8941 ctcgatcatt cctctgtcct caaacagggt cagtgtcagc acaatgtctt ccaggatatc
     9001 ctcattttcc tcgttgtcca ggaagtcttt atccttaatg atcttcagca gatcgtggta
     9061 agcgcccagg cttgcgttaa atctgtcttc gactccgctg atctccacgg aatcgaagca
     9121 ttcaattttc ttgaagtagt cctccttcag ctgcttgaca gtcactttcc tgttggtttt
     9181 aaacagcaga tccacgattg ctttcttctg ctctcctgac aggaaggctg gctttctcat
     9241 gccctcggtg acgtatttca cctttgtcag ttcgttatag acagtgaaat actcgtacag
     9301 cagggagtgc ttaggcagca ctttctcatt gggcaggttt ttgtcaaaat tagtcattct
     9361 ctcgatgaag gactgtgcag aggcgccctt atcgaccact tcctcgaagt tccaaggagt
     9421 aatggtttcc tcggatttgc gagtcatcca ggcaaatctg ctgtttccgc gagccagtgg
     9481 acccacatag taagggattc tgaaggtcag aattttttcg atcttctcgc ggttatcttt
     9541 cagaaagggg tagaagtctt cctggcgcct caggatagcg tgcagctctc ccagatgaat
     9601 ctggtgtggg atggaaccgt tatcaaatgt gcgctgcttc ctcagcaggt cctctctatt
     9661 cagtttcacc agcagttcct cagttccgtc catcttctcc agaataggtt tgataaactt
     9721 gtagaattcc tcctgtgatg cgcctccgtc gatataaccg gcgtagccgt tcttggactg
     9781 atcaaagaaa atttccttgt acttctctgg cagctgctgc ctgaccagag ccttcagcag
     9841 ggtcagatcc tgatggtgct cgtcatatct cttgatcatg ctggcactca gaggtgcttt
     9901 tgtaatctcg ctgttcactc tcagaatatc tgacagcagg attgcgtcgg acagattttt
     9961 ggcagccagg aacaggtcag cgtactgatc tccgatctgt gccagcaggt tatccaggtc
    10021 atcgtcatat gtgtcctttg acagctgcag tttggcgtcc tcagccagat caaaattgct
    10081 tttgaagtta ggagtcaggc ccagactcag agcgatcaga tttccaaaca gaccgttcct
    10141 cttttctccg ggcagctgtg caatcaggtt ctccagtctg cgtgacttgg acagcctggc
    10201 tgacaggata gcttttgcgt ccactctgga agcattaata gggttttcct caaacagctg
    10261 attgtaaatc tggaccagct ggatgaacag cttgtccaca tcggaattgt cggggttcag
    10321 atcgccctcg atcagaaaat gacccctgaa cttaatcatg tgggccagag ccagatagat
    10381 cagtctcagg tcggctttat cagtagagtc agccagtttc ttgcgcagat gatagatggt
    10441 tgggtatttt tcgtgatagg cgacctcgtc cacaatgttt ccaaagatag gatggcgctc
    10501 gtgtttctta tcttcctcga ccaggaaact ttcctccagc ctatgaaaga atgaatcgtc
    10561 caccttagcc atttcgttag agaaaatctc ctgcaggtaa cagattctat tcttccttct
    10621 ggtatagcgc cttcttgctg ttcttttcag gcgtgttgct tcggcagtct ctccagaacc
    10681 gaacagcagg gctccaatca gatttttctt gatgctgtgc ctgtcggtgt tacccagcac
    10741 cttaaatttc ttgctaggga ccttgtagtc gtctgtaatc actgcccaac ccacggagtt
    10801 agttccaata tccaggccga tgctatactt cttatccatg cttcccacct ttctcttctt
    10861 cttaggtgga gaagccatgg tggcgtcgac ctcgaggggg ggcccggtac ccaattcgcc
    10921 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaagcct gcttttttgt
    10981 acaaacttgc agtgaatctt tcccaagaat gatcacttgg atgagtccaa tggagagcac
    11041 agacagaacc ttcggtacac aatgtcagca agaatcaccg aagccaggct ttttcctttg
    11101 taagttattt tccgataaag gaatggtaca gactgggcaa tggacctgaa gattggaaca
    11161 aatgaaagtg tataagttac aattagttgc tgtggacagt atataatctc tgagaacaca
    11221 cactgatgta acttgcaaac ttgtatattc ctaactgttt gaaatagttt ctacaattga
    11281 atagtcccaa caccagtaca ttcagttctg ctcctgtagg gttctttcag ctaaacagag
    11341 tgttttgggc taaacaaaca tgaaccacct aatgttctgg tcttcaagat tgcaagaaac
    11401 atctcatcag gtgtgttggg gctaaactct gaaagaacgc gcggccctgc atgactacaa
    11461 ctggacatcc atgttctaat gggaaagtaa attagaatag ctcaaattta acaaggcttg
    11521 ttctttagaa tttagctcca ttctgccaaa gcacaacagg ccagagggtt ctagtaatct
    11581 gaaagaccta gatggcactt ttaaccctcg ccatcttctt ctgagtccac gataatagct
    11641 tatgactgta gaagattcta aagagatgct tagatttgct tgcacacaaa aaagattaaa
    11701 gtggggcact ctatggtgtc ggggacgtaa aggccttgtt cacgcactca aaaaaataat
    11761 aaaaatcact atttactttc ccttaagtgt ttacaaatct ctatgagttc ctttctctgc
    11821 tgaacataaa agaaaatatt tagaacaaag ctaaaatcca ttgacatcca tagtagttaa
    11881 aacagttact tgggaagtca atggttacag ggttacggct ttctttaaaa tatcttatta
    11941 tttattttca acaatagaaa gaaagccaaa taggcttgga acaagtaaag gatgaatcaa
    12001 taatgacaga atttttaatt tttgtggaaa ctcaacctgg aaaaatggaa caattgggaa
    12061 aaaaaaagtt taattataat tgttacaaag gtttgctgta atgttgtcct ccaaaaacat
    12121 gctggacacg aataaattaa gcataaaaac aacttctcag acaacatctg ccagaaaaaa
    12181 aattctatta ctaatataaa gttcacataa gtacctcaga gtgtacatcg gtggattggt
    12241 tttgggtaaa tatgatacag tgatgttcct ttgagtacta ttgcactgta aaagtacaat
    12301 tcatgttttt gtaaacatac aatcatataa catgtgtatt atttattaag aggactgcag
    12361 ttccttaaaa caacattgaa aaaaaactat tttctaactt cctgacatga aatatgatat
    12421 gatatgatca ttctttgagc cgaattcctt cctggttcag gtaaacagct gacaccaggt
    12481 gacttctggt actaatgcac gagtcgttta tctggtttag ttgttacgtg gttatgggtt
    12541 aaagataaaa tcacagaaag ataaggtcag taagtgactt tatcacacta gtcacaagtt
    12601 aagttgacaa ggtagggttt aatgccaccc actcactaaa tgactttcaa agtcatcaga
    12661 tttctgttcg ttccacatgg cctagctgtc tgtcgttacc gataatactt tcagtcaaaa
    12721 tacatttcac tctaaatgaa gtctaaatgc taaatacctc ttgagcaatg ggaaagcaaa
    12781 aataaatatc tccaaaaagt cattccagca taagtggaaa tatttcattc tattgttttt
    12841 atgttattct attgtactgc tgttttaatt tgcaaacccc aagcatccct ttaaatatta
    12901 atgtggtttt acacccaaat atttagccac tgttatgtac agttagcatt actggattca
    12961 ctgtactgta cgtccgagag aatgggtcct tttcacgctg tggtgggtgt agagctacgc
    13021 ctggtggggt gagtggatta tctcagaaaa acatgaggag tctctggaca caagaagctt
    13081 tctttgaaac attcagagtg ggacaagcag gctctggtct gtttatgtcc catctgaatg
    13141 ctgctataac tgtgctttat aggcatttag cctcaatgag aaaaacacac tttaaaaaga
    13201 gcaaaatatt taaccctgga ccacaaagat gggaataagg gtacattttc taaaattaag
    13261 gttttgcaca ttatctgaaa tgtggataaa ctgtccaaat gaatgtgatg tgattcattg
    13321 aataggccaa aatatttggc taagatacag ttaccactgt atatcccaaa ctaaaattaa
    13381 gtttttacat aattatggtt ggaaattcac agaaaattta tatggaacac aatatttgct
    13441 taacattgct attaaaaact gtaaattcat atattataat ttcatatgct cctaaaactt
    13501 acctgttttg tagcccaggg tcacatataa agttttttac tgagccatta acttcagatt
    13561 tccataaaaa agtgttacct gatgagtgac agtgaagact ttgaagatta tctccgacct
    13621 cagtctctca tctgctaatc ctctaggttc ttgttttgtg ctcctggatc cagagcttga
    13681 gccgaaatgc catagaaata tgatggaggg gtaacctgta gatggagatg ctcagctagg
    13741 gttgaatgtc acgtttggaa ggcgaggaca ggagtaaagt gctgccctct gggatgccag
    13801 tttgggtctg tgcagcagtg ggcagaagca caagcttatg tgtgtgtttg tgtcagtgtg
    13861 tgaatgcgtg aatgtgtctt gagtgtgtct cccagtgcct ggtgtaccag cacaatgact
    13921 cgctgttctc tcgccctcgc gttcctccat ccccagccca acccagccat ccagtcctct
    13981 ttttcatcct tcaacttctc gctctttcaa gtgaactcca tcttttctta ctacctttta
    14041 ttgattgcac tttcataaat gttcacagtg agaccggata caagaatttt aacgtccttc
    14101 tttcatgtct gtaggataat ggaatatgtg tgaaactata aataaaataa atatttaaat
    14161 aaatgattac aaaagcttac acatttttta aagataagtt caaaccgtat atggtttatt
    14221 taagacatag atcaccatat ttgattagtg taactaatat aaataataat aatatttaca
    14281 tttttacttt tagccgagaa tatctgagag gtgcttgcca acacaaaact caccctgcca
    14341 tgatggtttg aggttgtgga tagtcgccaa gatattgcta tgcattccct tgtggaaaaa
    14401 caagtacact ttatcagact ttagaacaaa acataatatt taaaaatgta tttcagtgca
    14461 gtctaaattt gttgtgtaat ttaaacaatt atttagttgt tgatgaaaat attttgtgta
    14521 acagtatttg aaaaattatt gcaactgtga ccagagcaca taagtagagc ttaagcacat
    14581 aattcatttg ttcaatatta catctactga aagcatttac agtataatta tggtgaacta
    14641 aaatatattt tatgccattt atattcaaac atatttgtca ggtttttaaa catattcatt
    14701 attgcattac atttgttatt aagaacgagt aatttggtac atttaaaata ttaacttaat
    14761 actaaataac ctactaaagt ttaaattata atcaagtact tcaatatttc agtcaacaaa
    14821 ctaaactaat cagatttttt taagcattag gaacgattat tgaagcataa tgatttactt
    14881 agaagtcaaa attttatttt gtcaatatta caaaacattt ttagaagtta tgatggttat
    14941 attcgtataa taaattaact ttttgctaaa aggggttgcc atgaaaatga tccttatcat
    15001 taaaaaaagc tgatatgtgc taaaaaaaga tcacagtttg aggtatgctt catgtaaata
    15061 gtaattaaaa agtaataaca ataataaata aaagatataa gtttaataat aacagacaac
    15121 tatatgttgc aatggtagtg ggagagaaag tgaaagctgt agtggcagca gattgttcag
    15181 atagcaactt ttctatacaa agttgatagc ttggcgtaat catggtcata gctgtttcct
    15241 gctcgagggc ccatctggcc tgtgtttcag acaccaggga gtctctgctc acgtttcctg
    15301 ctatttgcag cctctctatc aagactaata cacctcttcc cgcatcggct gcctgtgaga
    15361 ggcttttcag cactgcagga ttgcttttca gccccaaaag agctaggctt gacactaaca
    15421 attttgagaa tcagcttcta ctgaagttaa atctgaggtt ttacaacttt gagtagcgtg
    15481 tactggcatt agattgtctg tcttatagtt tgataattaa atacaaacag ttctaaagca
    15541 ggataaaacc ttgtatgcat ttcatttaat gttttttgag attaaaagct taaacaagaa
    15601 tctctagttt tctttcttgc ttttactttt acttccttaa tactcaagta caattttaat
    15661 ggagtacttt tttactttta ctcaagtaag attctagcca gatactttta cttttaattg
    15721 agtaaaattt tccctaagta cttgtacttt cacttgagta aaatttttga gtacttttta
    15781 cacctctgtc aagaactcct ggacaaacct ctgacctgtg tggaacagag tggatatggg
    15841 tgtctgaaca gatattcacg tcttttgcag atcagagggc atttctggtg ttgtgcaatg
    15901 acctgggtcc aacaggtagg cgtactcgta tccaagagca ttgttggaca ggaggtagtc
    15961 tccattccta taaagaggaa ggaatggcac catgtagtag ccgtcattgt ggccaattgg
    16021 tgcattggta cgtgggtaga tggaccgggg aggctgatga gttcttagcc agcctttccc
    16081 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    16141 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    16201 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    16261 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    16321 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    16381 atattaacgc ttacaattta ggtggcac
//
