LOCUS       sequence-391179-        9991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: beta-catenin (S33A, S37A, T41A, S45A)-pcw107
FEATURES             Location/Qualifiers
     source          1..9991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    522..546
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             609..644
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     protein_bind    complement(2911..2935)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2943..2984
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3009..3597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3062..3082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3480..3491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3669..3902
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3980..4101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4017..4036)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4071..4090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4141..4276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4296..4315)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4297..4315)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4325..4342)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4325..4341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4334..4356)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4483..4938
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4570..4589)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4780..4801
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4964..5068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5069..5137,5138..5929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5287..5306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6100..6688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6589..6608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6842..6859
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6976..6997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7012..7017,7018..7035,7036..7042)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7050..7066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7055..7077
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7074..7090
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7074..7090
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7109..7129
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7111..7129
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7157..7383
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7384..7564
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7611..7736
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8229..8462
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8647..8691
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8922..9132
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8944..9140
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8991..9126
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9172..9774
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9172..9191)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9668..9688
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9817..9934
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca ggggctagca tcgatggatc aacaagtttg tacaaaaaag
      541 ttggcaaatg cggcggcggc accatggcta ctcaagctga cctgatggag ttggacatgg
      601 ccatggagcc ggacagaaaa gctgctgtca gccactggca gcagcagtct tacttggatg
      661 ctggaatcca tgctggtgcc accgccacag ctcctgccct gagtggcaag ggcaaccctg
      721 aggaagaaga tgttgacacc tcccaagtcc tttatgaatg ggagcaaggc ttttcccagt
      781 ccttcacgca agagcaagta gctgatattg acgggcagta tgcaatgact agggctcaga
      841 gggtccgagc tgccatgttc cctgagacgc tagatgaggg catgcagatc ccatccacgc
      901 agtttgacgc tgctcatccc actaatgtcc agcgcttggc tgaaccatca cagatgttga
      961 aacatgcagt tgtcaatttg attaactatc aggatgacgc ggaacttgcc acacgtgcaa
     1021 ttcctgagct gacaaaactg ctaaacgatg aggaccaggt ggtagttaat aaagctgctg
     1081 ttatggtcca tcagctttcc aaaaaggaag cttccagaca tgccatcatg cgctcccctc
     1141 agatggtgtc tgccattgta cgcaccatgc agaatacaaa tgatgtagag acagctcgtt
     1201 gtactgctgg gactctgcac aacctttctc accaccgcga gggcttgctg gccatcttta
     1261 agtctggtgg catcccagcg ctggtgaaaa tgcttgggtc accagtggat tctgtactgt
     1321 tctacgccat cacgacactg cataatctcc tgctccatca ggaaggagct aaaatggcag
     1381 tgcgcctagc tggtggactg cagaaaatgg ttgctttgct caacaaaaca aacgtgaaat
     1441 tcttggctat tacaacagac tgccttcaga tcttagctta tggcaatcaa gagagcaagc
     1501 tcatcattct ggccagtggt ggaccccaag ccttagtaaa cataatgagg acctacactt
     1561 atgagaagct tctgtggacc acaagcagag tgctgaaagt gctgtctgtc tgctctagca
     1621 acaagccggc cattgtagaa gctggtggga tgcaggcact ggggcttcat ctgacagacc
     1681 caagtcagcg acttgttcaa aactgtcttt ggactctcag aaacctttca gatgcagcga
     1741 ctaagcagga agggatggaa ggcctccttg ggactctagt gcagcttctg ggttccgatg
     1801 atataaatgt ggtcacctgt gcagctggaa ttctctctaa cctcacttgc aataattaca
     1861 aaaacaagat gatggtgtgc caagtgggtg gcatagaggc tcttgtacgc accgtccttc
     1921 gtgctggtga cagggaagac atcactgagc ctgccatctg tgctcttcgt catctgacca
     1981 gccggcatca ggaagccgag atggcccaga atgccgttcg ccttcattat ggactgcctg
     2041 ttgtggttaa actcctgcac ccaccatccc actggcctct gataaaggca actgttggat
     2101 tgattcgaaa ccttgccctt tgcccagcaa atcatgcgcc tttgcgggaa cagggtgcta
     2161 ttccacgact agttcagctg cttgtacgag cacatcagga cacccaacgg cgcacctcca
     2221 tgggtggaac gcagcagcag tttgtggagg gcgtgcgcat ggaggagata gtagaagggt
     2281 gtactggagc tctccacatc cttgctcggg acgttcacaa ccggattgta atccgaggac
     2341 tcaataccat tccattgttt gtgcagttgc tttattctcc cattgaaaat atccaaagag
     2401 tagctgcagg ggtcctctgt gaacttgctc aggacaagga ggctgcagag gccattgaag
     2461 ctgagggagc cacagctccc ctgacagagt tactccactc caggaatgaa ggcgtggcaa
     2521 catacgcagc tgctgtccta ttccgaatgt ctgaggacaa gccacaggat tacaagaagc
     2581 ggctttcagt cgagctgacc agttccctct tcaggacaga gccaatggct tggaatgaga
     2641 ctgcagatct tggactggac attggtgccc agggagaagc ccttggatat cgccaggatg
     2701 atcccagcta ccgttctttt cactctggtg gatacggcca ggatgccttg gggatggacc
     2761 ctatgatgga gcatgagatg ggtggccacc accctggtgc tgactatcca gttgatgggc
     2821 tgcctgatct gggacacgcc caggacctca tggatgggct gcccccaggt gatagcaatc
     2881 agctggcctg gtttgatact gacctgtaat tgccaacttt cttgtacaaa gtggttaccg
     2941 gtggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaccgcgtt
     3001 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3061 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3121 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3181 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3241 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3301 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3361 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3421 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3481 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3541 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     3601 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3661 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     3721 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     3781 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     3841 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3901 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     3961 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     4021 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4081 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     4141 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4201 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4261 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     4321 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4381 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4441 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     4501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4681 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4801 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4861 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4921 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4981 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5041 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5101 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5161 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5221 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5281 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5341 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5401 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5461 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5521 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5581 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5641 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5701 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5761 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5821 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5881 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5941 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6001 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6061 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6121 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6181 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6241 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6301 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6361 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6421 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6481 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6541 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6601 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6661 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6721 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6781 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6841 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6901 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6961 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7021 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7081 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     7141 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     7201 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     7261 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     7321 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     7381 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7441 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7501 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7561 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     7621 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     7681 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     7741 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     7801 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     7861 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     7921 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     7981 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8041 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     8101 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     8161 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     8221 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     8281 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     8341 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     8401 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     8461 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     8521 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     8581 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     8641 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     8701 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     8761 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     8821 tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca
     8881 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8941 gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9001 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9061 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9121 gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag
     9181 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     9241 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     9301 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     9361 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     9421 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     9481 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     9541 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     9601 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     9661 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     9721 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt
     9781 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     9841 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     9901 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     9961 tccactttgg cgccggctcg aggcctgcag g
//
