LOCUS       sequence-391181-        9988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..9988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    522..546
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             609..644
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     protein_bind    complement(2908..2932)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2940..2981
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3006..3594
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3059..3079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3477..3488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3666..3899
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3977..4098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4014..4033)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4068..4087
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4138..4273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4293..4312)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4294..4312)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4322..4339)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4322..4338)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4331..4353)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4480..4935
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4567..4586)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4777..4798
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4961..5065
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5066..5134,5135..5926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5284..5303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6097..6685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6586..6605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6839..6856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6973..6994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7009..7014,7015..7032,7033..7039)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7047..7063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7052..7074
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7071..7087
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7071..7087
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7106..7126
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7108..7126
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7154..7380
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7381..7561
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7608..7733
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8226..8459
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8644..8688
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8919..9129
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8941..9137
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8988..9123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9169..9771
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9169..9188)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9665..9685
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9814..9931
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca ggggctagca tcgatggatc aacaagtttg tacaaaaaag
      541 ttggcaaatg cggcggcggc accatggcta ctcaagctga cctgatggag ttggacatgg
      601 ccatggagcc ggacagaaaa gctgctgtca gccactggca gcagcagtct tacttggatg
      661 ctggaatcca tgctggtgcc accgccacag ctcctgccct gagtggcaag ggcaaccctg
      721 aggaagaaga tgttgacacc tcccaagtcc tttatgaatg ggagcaaggc ttttcccagt
      781 ccttcacgca agagcaagta gctgatattg acgggcagta tgcaatgact agggctcaga
      841 gggtccgagc tgccatgttc cctgagacgc tagatgaggg catgcagatc ccatccacgc
      901 agtttgacgc tgctcatccc actaatgtcc agcgcttggc tgaaccatca cagatgttga
      961 aacatgcagt tgtcaatttg attaactatc aggatgacgc ggaacttgcc acacgtgcaa
     1021 ttcctgagct gacaaaactg ctaaacgatg aggaccaggt ggtagttaat aaagctgctg
     1081 ttatggtcca tcagctttcc aaaaaggaag cttccagaca tgccatcatg cgctcccctc
     1141 agatggtgtc tgccattgta cgcaccatgc agaatacaaa tgatgtagag acagctcgtt
     1201 gtactgctgg gactctgcac aacctttctc accaccgcga gggcttgctg gccatcttta
     1261 agtctggtgg catcccagcg ctggtgaaaa tgcttgggtc accagtggat tctgtactgt
     1321 tctacgccat cacgacactg cataatctcc tgctccatca ggaaggagct aaaatggcag
     1381 tgcgcctagc tggtggactg cagaaaatgg ttgctttgct caacaaaaca aacgtgaaat
     1441 tcttggctat tacaacagac tgccttcaga tcttagctta tggcaatcaa gagagcaagc
     1501 tcatcattct ggccagtggt ggaccccaag ccttagtaaa cataatgagg acctacactt
     1561 atgagaagct tctgtggacc acaagcagag tgctgaaagt gctgtctgtc tgctctagca
     1621 acaagccggc cattgtagaa gctggtggga tgcaggcact ggggcttcat ctgacagacc
     1681 caagtcagcg acttgttcaa aactgtcttt ggactctcag aaacctttca gatgcagcga
     1741 ctaagcagga agggatggaa ggcctccttg ggactctagt gcagcttctg ggttccgatg
     1801 atataaatgt ggtcacctgt gcagctggaa ttctctctaa cctcacttgc aataattaca
     1861 aaaacaagat gatggtgtgc caagtgggtg gcatagaggc tcttgtacgc accgtccttc
     1921 gtgctggtga cagggaagac atcactgagc ctgccatctg tgctcttcgt catctgacca
     1981 gccggcatca ggaagccgag atggcccaga atgccgttcg ccttcattat ggactgcctg
     2041 ttgtggttaa actcctgcac ccaccatccc actggcctct gataaaggca actgttggat
     2101 tgattcgaaa ccttgccctt tgcccagcaa atcatgcgcc tttgcgggaa cagggtgcta
     2161 ttccacgact agttcagctg cttgtacgag cacatcagga cacccaacgg cgcacctcca
     2221 tgggtggaac gcagcagcag tttgtggagg gcgtgcgcat ggaggagata gtagaagggt
     2281 gtactggagc tctccacatc cttgctcggg acgttcacaa ccggattgta atccgaggac
     2341 tcaataccat tccattgttt gtgcagttgc tttattctcc cattgaaaat atccaaagag
     2401 tagctgcagg ggtcctctgt gaacttgctc aggacaagga ggctgcagag gccattgaag
     2461 ctgagggagc cacagctccc ctgacagagt tactccactc caggaatgaa ggcgtggcaa
     2521 catacgcagc tgctgtccta ttccgaatgt ctgaggacaa gccacaggat tacaagaagc
     2581 ggctttcagt cgagctgacc agttccctct tcaggacaga gccaatggct tggaatgaga
     2641 ctgcagatct tggactggac attggtgccc agggagaagc ccttggatat cgccaggatg
     2701 atcccagcta ccgttctttt cactctggtg gatacggcca ggatgccttg gggatggacc
     2761 ctatgatgga gcatgagatg ggtggccacc accctggtgc tgactatcca gttgatgggc
     2821 tgcctgatct gggacacgcc caggacctca tggatgggct gcccccaggt gatagcaatc
     2881 agctggcctg gtttgatact gacctgttgc caactttctt gtacaaagtg gttaccggtg
     2941 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga ccgcgttaag
     3001 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3061 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3121 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3181 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3241 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3301 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3361 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3421 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3481 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3541 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     3601 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3661 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     3721 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     3781 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     3841 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     3901 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     3961 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4021 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4081 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     4141 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4201 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4261 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     4321 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     4381 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     4441 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     4501 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4561 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4621 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4681 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4741 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4801 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4861 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4921 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4981 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5041 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5101 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5161 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5221 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5281 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5341 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5401 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5461 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5521 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5581 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5641 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5701 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5761 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5821 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5881 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5941 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6001 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6061 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6241 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6481 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6781 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6841 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6901 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6961 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7021 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     7081 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     7141 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     7201 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     7261 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     7321 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     7381 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7441 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7501 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7561 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     7621 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     7681 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     7741 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     7801 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     7861 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     7921 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     7981 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     8041 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     8101 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     8161 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     8221 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     8281 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     8341 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     8401 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     8461 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     8521 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     8581 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     8641 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     8701 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     8761 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     8821 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt
     8881 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8941 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     9001 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9061 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9121 gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac
     9181 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     9241 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     9301 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9361 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     9421 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9481 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9541 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     9601 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     9661 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9721 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta
     9781 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     9841 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9901 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     9961 actttggcgc cggctcgagg cctgcagg
//
