LOCUS       sequence-39120-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Lin CW, Ting AY
  TITLE     A genetically encoded fluorescent reporter of histone 
            phosphorylation in living cells.
  JOURNAL   Angew Chem Int Ed Engl. 2004 May 24;43(22):2940-3.
  PUBMED    15170310
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl."; date: "2004-05-24"; volume: "43(22)"; pages: "2940-3"
COMMENT     Sequence Label: pcDNA3-S28 histone 3 phosphorylation reporter-NLS
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..87)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(88..339)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(130..151)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     479..498
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga attcttaagc
       61 gtcttccacc tttctcttct tttttggctt gtacagctcg tccatgccga gagtgatccc
      121 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
      181 cagggcggac tggtagctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
      241 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
      301 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
      361 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      421 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      481 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
      541 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcacatcag
      601 gccgtagccg aaggtggtca cgagggtggg ccagggcacg
//
