LOCUS       sequence-391202-       10630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pVS1-based T-DNA binary vector with NPTII as a plant selectable 
            marker gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Lee K, Kang M, Ji Q, Grosic S, Wang K
  TITLE     New T-DNA binary vectors with NptII selection and RUBY reporter for 
            efficient maize transformation and targeted mutagenesis.
  JOURNAL   Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231.
  PUBMED    37070560
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231."
COMMENT     Sequence Label: pCBL101
FEATURES             Location/Qualifiers
     source          1..10630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        387..2381
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2410..2457
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2477..3271
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2531..2550)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3141..3160
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3838..3855)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3838..3854)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3847..3869)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4069..4093
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4614..5405
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5651..6239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6140..6159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6393..6410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6425..6565
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6491..6510)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6651..6673
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6909..7103
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7169..8242)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8671..9300)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaata
      361 gttccggact taccggcgcc gctagcctgc agtgcagcgt gacccggtcg tgcccctctc
      421 tagagataat gagcattgca tgtctaagtt ataaaaaatt accacatatt ttttttgtca
      481 cacttgtttg aagtgcagtt tatctatctt tatacatata tttaaacttt actctacgaa
      541 taatataatc tatagtacta caataatatc agtgttttag agaatcatat aaatgaacag
      601 ttagacatgg tctaaaggac aattgagtat tttgacaaca ggactctaca gttttatctt
      661 tttagtgtgc atgtgttctc cttttttttt gcaaatagct tcacctatat aatacttcat
      721 ccattttatt agtacatcca tttagggttt agggttaatg gtttttatag actaattttt
      781 ttagtacatc tattttattc tattttagcc tctaaattaa gaaaactaaa actctatttt
      841 agttttttta tttaataatt tagatataaa atagaataaa ataaagtgac taaaaattaa
      901 acaaataccc tttaagaaat taaaaaaact aaggaaacat ttttcttgtt tcgagtagat
      961 aatgccagcc tgttaaacgc cgtcgacgag tctaacggac accaaccagc gaaccagcag
     1021 cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc tctggacccc
     1081 tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg
     1141 gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg
     1201 cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat
     1261 aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac
     1321 acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc
     1381 gtcctccccc cccccctctc taccttctct agatcggcgt tccggtccat gcatggttag
     1441 ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt
     1501 gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt
     1561 gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga
     1621 tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa
     1681 tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg
     1741 atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc
     1801 tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat
     1861 tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac
     1921 tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc
     1981 ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat
     2041 taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg
     2101 gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg
     2161 atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa
     2221 caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc
     2281 tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct
     2341 tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca ggtcgactct agaggatcaa
     2401 ttcgctagcg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcaga
     2461 tccaccggga tccaccatgg ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2521 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2581 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     2641 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     2701 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     2761 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     2821 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     2881 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     2941 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3001 ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3061 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3121 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3181 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3241 cttctatcgc cttcttgacg agttcttctg aggatccacc atggttaacc tagacttgtc
     3301 catcttctgg attggccaac ttaattaatg tatgaaataa aaggatgcac acatagtgac
     3361 atgctaatca ctataatgtg ggcatcaaag ttgtgtgtta tgtgtaatta ctagttatct
     3421 gaataaaaga gaaagagatc atccatattt cttatcctaa atgaatgtca cgtgtcttta
     3481 taattctttg atgaaccaga tgcatttcat taaccaaatc catatacata taaatattaa
     3541 tcatatataa ttaatatcaa ttgggttagc aaaacaaatc tagtctaggt gtgttttgca
     3601 agcttgcatg cctgcagtcg tctattggac cgacagttgc tattaatata atgggccacc
     3661 atagtagact gacaaataaa ttacctgaca acatcgtttc acaaaaaaac aaacacaaaa
     3721 agggagtgca ttttccaggg catttttgta ataaaaaaca gttaaaaggg agtgcaatag
     3781 aaatataggg gtgtggaaat agtgatttga gcacgtcttg aagcgaatta gcttggcact
     3841 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3901 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3961 ttcccaacag ttgcgcagcc tgaatggcga atgctagagc aattcggcgt taattcagta
     4021 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     4081 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     4141 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     4201 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     4261 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     4321 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     4381 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     4441 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     4501 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     4561 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     4621 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     4681 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     4741 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     4801 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     4861 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     4921 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     4981 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     5041 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     5101 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     5161 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     5221 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     5281 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     5341 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     5401 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     5461 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     5521 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     5581 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5641 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5701 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5761 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     5821 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5881 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5941 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6001 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6061 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6121 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6181 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6241 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6301 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6361 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6421 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     6481 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     6541 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     6601 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     6661 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     6721 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     6781 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     6841 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     6901 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     6961 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     7021 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     7081 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     7141 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     7201 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     7261 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     7321 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     7381 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     7441 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     7501 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     7561 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     7621 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     7681 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     7741 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     7801 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     7861 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     7921 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     7981 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     8041 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     8101 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     8161 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     8221 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     8281 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
     8341 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
     8401 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
     8461 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
     8521 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
     8581 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
     8641 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
     8701 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
     8761 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
     8821 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
     8881 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
     8941 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
     9001 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
     9061 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
     9121 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
     9181 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
     9241 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
     9301 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
     9361 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
     9421 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
     9481 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
     9541 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
     9601 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
     9661 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
     9721 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
     9781 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
     9841 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
     9901 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
     9961 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    10021 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    10081 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    10141 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    10201 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    10261 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    10321 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    10381 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    10441 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa
    10501 agtactttaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
    10561 gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct
    10621 tttctcttag
//
