LOCUS       sequence-391203-        2444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-35S-11
FEATURES             Location/Qualifiers
     source          1..2444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    100..274
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(324..342)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        410..514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             736..1527
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1698..2286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2187..2206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2440..2444,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttctcgagt ttctccataa taatgtgtga
      121 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
      181 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
      241 cctaaaacca aaatccagta ctaaaatcca gatcccccga attacgcttg agaccacgaa
      301 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      361 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      421 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      481 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      541 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      601 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      661 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      721 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      781 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      841 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      901 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      961 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     1021 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     1081 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     1141 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     1201 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     1261 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1321 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1381 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1441 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1501 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1561 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1621 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1681 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1741 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1801 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1861 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1921 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2041 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2281 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2341 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2401 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcga
//
