LOCUS       sequence-391204-        2769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-HpaC-9
FEATURES             Location/Qualifiers
     source          1..2769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(649..667)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        735..839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1061..1852
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2023..2611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2512..2531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2765..2769,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgcaattag acgagcagcg tcttagattt
      121 cgtgatgcta tggcttctct ttctgccgct gttaatatca ttacaacgga aggcgacgca
      181 ggtcaatgcg gcatcactgc cactgctgtc tgcagcgtca cggacacgcc accgtctttg
      241 atggtttgca taaatgccaa ttcagcaatg aaccctgtgt tccaaggtaa cggcaagctc
      301 tgtgtaaatg ttctcaatca cgagcaagag ttaatggcac gtcactttgc agggatgacc
      361 gggatggcaa tggaggaacg tttttccctc tcttgctggc aaaaaggccc gctggctcaa
      421 cctgtactga aaggatcact cgcctccctt gagggggaaa tcagagatgt gcaggctatt
      481 ggcacacacc tcgtgtacct cgttgagatt aagaacataa ttttaagcgc agagggccac
      541 gggctgatct actttaagag gcgatttcat ccagttatgc tggaaatgga ggctgcaatc
      601 agtggctgag ctttgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
      661 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      721 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      781 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      841 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
      901 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
      961 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     1021 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     1081 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     1141 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     1201 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     1261 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     1321 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     1381 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     1441 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     1501 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     1561 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     1621 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     1681 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     1741 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     1801 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
     1861 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1921 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2161 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2641 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2701 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2761 gcgcagcga
//
