LOCUS       sequence-391206-        3819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-HpaB-9
FEATURES             Location/Qualifiers
     source          1..3819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1699..1717)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1785..1889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2111..2902
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3073..3661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3562..3581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3815..3819,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgaaacccg aggacttcag ggcctcaaca
      121 caaagaccct ttacaggtga agaatacctt aagtctctcc aggacggacg agagatatat
      181 atatacgggg aaagggtcaa agacgttaca acccatcccg ccttcagaaa tgctgccgcc
      241 tctgtggctc agctctatga tgccttgcat aaaccggaaa tgcaagactc attatgttgg
      301 aatacggata ctggctctgg cggttacacc cataaattct ttagagtcgc aaagtcagct
      361 gacgatttac gtcagcagcg agatgcaatt gccgagtggt ccaggttgtc ttatggttgg
      421 atggggcgta cccctgacta taaggccgcc tttggatgtg ccttgggtgc aaacccaggg
      481 ttttatggcc agttcgagca gaatgcccga aactggtaca ccaggatcca agaaaccggg
      541 ctctacttca accacgcaat cgttaatcct ccgatcgaca gacacctccc tacggacaag
      601 gtgaaagatg tttatataaa gttggagaaa gagacagatg caggaattat cgttagcgga
      661 gctaaggtcg tggccactaa ctccgctctc acacactata atatgatcgg atttggatct
      721 gctcaagtca tgggagaaaa tcctgacttt gctcttatgt ttgttgctcc aatggatgct
      781 gatggcgtta aattgatctc acgagcttcc tacgaaatgg tagctggggc cacgggtagt
      841 ccctacgact atcccttgtc ctcccgattt gatgaaaatg atgccatctt ggtcatggac
      901 aatgtcctta ttccatggga aaacgttttg atctaccgag atttcgaccg atgtaggcga
      961 tggaccatgg agggcggctt cgccagaatg taccctttac aagcatgcgt ccgtcttgcc
     1021 gttaaactcg actttataac cgccttgttg aaaaagtcat tagaatgcac cggcaccctc
     1081 gaatttcgag gagtccaagc tgacctgggg gaggttgtgg catggcgaaa cacattttgg
     1141 gccctcagtg actcaatgtg cagcgaggcc actccgtggg taaacggcgc ctatctgccg
     1201 gaccatgccg cattgcaaac gtaccgtgtg ctcgcaccta tggcctatgc taagataaag
     1261 aacattatag aacgtaatgt tacaagcgga ctgatctatc tcccaagctc cgccagggat
     1321 ctcaacaatc cccaaataga ccaatatctt gcaaaatacg taaggggatc aaacgggatg
     1381 gatcacgttc aaaggattaa aatcttaaag ctcatgtggg acgcaatcgg gtctgagttc
     1441 ggtgggagac acgagcttta cgagattaac tatagcggtt ctcaggatga gataaggctt
     1501 cagtgtctga gacaggcaca aaacagtggc aatatggata aaatgatggc aatggtcgat
     1561 aggtgtttga gtgagtacga ccaggatgga tggacggtcc cccatctcca caataatgat
     1621 gacattaata tgttagataa attgctgaag tccggctgag ctttgagacc acgaagtggc
     1681 tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1741 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1801 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1861 tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga
     1921 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1981 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     2041 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     2101 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     2161 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     2221 agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag
     2281 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     2341 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     2401 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     2461 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     2521 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     2581 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     2641 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2701 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2761 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     2821 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat
     2881 caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga
     2941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3781 tgataccgct cgccgcagcc gaacgaccga gcgcagcga
//
