LOCUS       sequence-391208-        7347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-NnHISPS-9
FEATURES             Location/Qualifiers
     source          1..7347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5227..5245)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5313..5417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5639..6430
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6601..7189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7090..7109
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(7343..7347,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgaatagta gtaaaaaccc tccatccact
      121 ctgttagatg tttttctgga taccgctagg aaccttgata cagcatccag aaatgtcctg
      181 gagtgcggag aacacaggtg gagttacaga gagttggaca cagtgtcatc cgcattggcc
      241 caacaccttc gatacactgt gggtcttagc cccactgtag ccgtgattag cgagaatcac
      301 ccctacatac ttgcattaat gctcgccgta tggaaactcg gcggaacgtt cgcaccgata
      361 gatgttcatt ccccagccga acttgttgct ggaatgttga atattgtaag cccgtcatgt
      421 cttgtcattc ctagctctga tgtgacaaat cagacattgg catgcgatct taatataccg
      481 gttgttgcat tccacccaca tcaaagtaca attcccgaac tcaacaagaa gtatctcaca
      541 gattctcaga tcagtcctga cctgcccttc cccgacccta atcgacctgc tttgtactta
      601 ttcacgtcat ctgcaacgtc taggtccaat ttgaagtgcg tgccacttac acacacattc
      661 atattgcgaa actccttaag taaacgagca tggtgcaaaa gaatgaggcc agagacagat
      721 tttgatggta ttagggtcct ggggtgggcc ccctggagcc atgtcctcgc tcatatgcag
      781 gacattggcc ctcttacatt attgaatgcc gggtgctacg tatttgccac gaccccatct
      841 acctacccga cggagctgaa ggatgaccgt gatgttatta gttgcgccgc aaatgccgta
      901 atgtacaaag gtgttaaatc cttcgcatgc ctcccattcg tgcttggggg actcaaggct
      961 ttatgtgagt cagagcccag tgttaaggca caactgcaag tggaggaacg agcccagctt
     1021 ttgaagtcac tccaacacat gaaaatcctg gaatgcgggg gggcaatgct cgaagtgagt
     1081 gtggcaagct gggctataga gaataggatt cctatatcta ttgggattgg tatgacagag
     1141 acaggcggcg ctcttttcgc tgggccggtc caagcaattc aaaccggctt tagcagtgaa
     1201 gataaattta tcgaggacgc aacctacttg ctggtcaagg acgattacga gagccacgca
     1261 gaggaggaca taaacgaggg cgagttagtg gtaaagtcca gaatgttacc taggggttat
     1321 ttgggctata acgacccctc tttctctgtt gacgatgctg gttgggttac tttcaaaact
     1381 ggagatcgat attcagtcac tcctgacggc aaattctcct ggcttggtcg aaatactgat
     1441 ttcattcaga tgacgagtgg tgagactctc gatccccgtc cgattgagtc cctcctttgt
     1501 gagtccagcc ttatatcacg agcctgtgtc atcggggata agttcctgaa cggaccagcc
     1561 acagctgtat gcgctattat agaactcgaa ccaacgactg ttgagaaagg ccaggctcac
     1621 agcagggata ttgcccgaat ctttgccccc atcaatcgag atcttcctcc cccgttacgt
     1681 atagcatgga gtcacgtact tgtacttcag ccaagcgaga aaataccgat gacgaaaaag
     1741 ggtacaatct tccgtaagaa aatcgaacag gttttcggtt ccgctttagg aggcagtagc
     1801 ggcgacaact cacaagcaac cactgacgct tcagtagtta ggagggatga gttgagcaac
     1861 acggtcaaac atatcataag cagagttttg ggagtctccg acgatgaact cctttggaca
     1921 ctgagctttg ctgagcttgg catgacgtcc gctcttgcta ctagaatagc aaatgaattg
     1981 aacgaagttc tggtaggtgt gaacctcccc atcaatgcat gctacattca tgtggacctg
     2041 ccttctttgt caaatgccgt gtatgcaaag ttagcacacc ttaaactccc ggataggacg
     2101 cccgagccaa ggaaggcccc agttgaaaat cccggtggaa aggaaatcgt aatagtaggt
     2161 caggctttta ggcttccggg gagtataaat gatgtcgctt ctttgaggga cgcttttttg
     2221 gctagacaag catccagtat tattactgaa ataccgccag atagatggga ccacgcatca
     2281 ttttatccaa aagacatacg atttaataaa gcagggctcg tcgacatcgc taactacgac
     2341 cacagttttt tcggattgac agccacggag gcactgtacc tgtcaccaac gatgcgtttg
     2401 gcacttgaag tgtcctttga agccttggag aatgctaata tacccgtgtc ccagttaaag
     2461 ggctctcaaa cagctgtata tgtcgcaacc accgacgacg ggtttgagac actgctgaat
     2521 gctgaggcag gatatgatgc atacacacga ttctatggca ctggtagggc cgcctctacg
     2581 gccagtggac gaattagtta tttactcgac gtacacggcc cttcaattac cgtagacact
     2641 gcttgttcag gcggtgctgt ttgcatagac caggccatag actacctcca atcctctagc
     2701 gcagctgata ccgccataat ctgcgccagc aacactcact gctggccagg ttccttcatg
     2761 tttctgagtg ctcaaggtat ggtaagttcc ggggggcgat gtgcaacttt tacgacggat
     2821 gccgacggct atgtaccctc tgaaggtgct gtggcattta tcctcaaaac ccgagaagca
     2881 gctatgagag ataaagatac tatcttggca actatcaaag caacgcagat tagtcataat
     2941 ggtcgttccc aaggtttagt cgcaccaaac gtcaattcac aagctgattt acacagaagc
     3001 ttattgcaga aagctggttt gagtccagcc gacattcact tcatcgaagc tcacgggaca
     3061 ggaacgtcac tcggtgacct tagtgaaata caagcaatta atgatgccta cacgtcatct
     3121 cagcctcgaa cagccggacc cttaatagta agcgcaagta aaaccgtcat tggtcacaca
     3181 gagcctgctg gaccgttggt aggcatgctc tcagttctca actcctttaa agagggcgca
     3241 gttcccggct tagctcacct gacagcagat aacctcaatc cggctttaga ctgctcctcc
     3301 gtccctttac ttatccccta ccagcctgtc cacttggcag ctcctaagcc acacagggct
     3361 gctgtgatga gttatggctt ctccggcact cttggaggta tagttcttga agcaccggat
     3421 gaggaaaggc ttgaggaaga accacccaac gataagccta tgttatttgt tgttagcgcc
     3481 aaaactcata ctgcattgat tgagtacctt ggacgatacc ttgaatttct gctccaggcc
     3541 aatccccaag acttctgcga tatctgttac actagctgcg ttggacgtga gcattacagg
     3601 taccgttttg catgtgtggc aaacgatatg gaagatttaa ttggccaact tcagaagagg
     3661 cttgggtcta aggttccacc taaaccgtcc tataaaagag gagccctcgc ttttgctttc
     3721 agtggacaag ggacccagtt tcgtggaatg gcaacagaat tggctaaggc atatagtggt
     3781 tttcgtaaga tagtcagtga tcttgctaaa agagcatcag aactgtccgg ccatgcaatc
     3841 gacaggtttc tgcttgcata tgacattggg gctgagaatg ttgcaccgga tagtgaagct
     3901 gatcaaatct gcatattcgt ttaccaatgt agtgttctca gatggctcca aacaatgggg
     3961 attcgtccgt cagccgtgat cggtcacagc ctcggtgaga ttagcgcatc cgtagctgct
     4021 ggagcattgt ccttggactc tgcccttgac ctcgtaataa gtagagccag gttactgagg
     4081 tcatctacga atgccccagc tggcatggca gctatgtctg catcccagga tgaagtagtc
     4141 gaactcatag gaaagctcga tttagataaa gctaatagcc tcagcgtgtc cgtaataaac
     4201 ggcccccaga acaccgttgt ttcaggatct tctgcagcaa tagaaagtat cgttgctctc
     4261 gcaaagggac gaaaaatcaa ggccagcgca ctcaacatta accaggcatt ccatagtcct
     4321 tacgtcgatt ctgcagtacc cggcttacga gcttggagtg agaaacatat atcatcagct
     4381 cgaccattgc aaatcccttt gtactctacc ctcttgggcg ctcaagtttc tgagggtcaa
     4441 atgttaaatc ccgatcactg ggtagaccat gctaggaaac ccgttcagtt cgcacaagca
     4501 gccactataa tgaaggaatc tttcaccgga gtaataatcg atattggacc tcaagttgta
     4561 gcatggtccc tgctcttaag caatggtctt acttccgtga ccgcactcgc cgcaaagcgt
     4621 ggccgttcac agcaagtggc cttcctctcc gccttagctg atctttatca ggactacggc
     4681 gtcgtaccag actttgttgg actgtacgca caacaggagg acgcttccag actgaaaaag
     4741 accgatatct taacttaccc ctttcagagg gttcgacgtt acccttcctt tataccctct
     4801 agaagggcac ccacccacgc tcatgtccaa gacgaggaaa ctctgtcatc aggatcatca
     4861 actccaacct tagaaaatac tgacctggac agtgggaagg aatctctgat gggaccaaca
     4921 agagggcttt tgcgagtcga tgatcttagg gactcaatag tcagttcagt caaagatgtc
     4981 ctggaattga agagtaacga agatctggac ctcagcgagt cccttaatgc tcttggcatg
     5041 gactctataa tgttcgctca attacgaaaa aggatagggg agggcctcgg cctgtccgtt
     5101 ccaatggttt ttctcagcga tgccttcagt attggagaaa tggtcagtaa cctcgtggaa
     5161 caggccgaag cttccgagga caattgagct ttgagaccac gaagtggctc ttcagtggac
     5221 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5281 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5341 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5401 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     5461 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
     5521 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     5581 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     5641 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     5701 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     5761 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     5821 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     5881 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     5941 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     6001 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     6061 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     6121 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     6181 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     6241 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     6301 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     6361 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     6421 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6481 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6721 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6781 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6841 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6901 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6961 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7021 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7081 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7141 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7201 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7261 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7321 ccgcagccga acgaccgagc gcagcga
//
