LOCUS       sequence-391209-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Boost the expression of pnRFP in mammalian cells (e.g., macrophage 
            RAW 264.7 cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Pang Y, Huang M, Fan Y, Yeh HW, Xiong Y, Ng HL, Ai HW
  TITLE     Development, Characterization, and Structural Analysis of a 
            Genetically Encoded Red Fluorescent Peroxynitrite Biosensor.
  JOURNAL   ACS Chem Biol. 2023 Jun 16;18(6):1388-1397. doi: 
            10.1021/acschembio.3c00139. Epub 2023 May 15.
  PUBMED    37185019
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.3c00139"; journalName: "ACS Chem Biol"; date: 
            "2023-06-16- 16"; volume: "18"; issue: "6"; pages: "1388-1397"
COMMENT     Sequence Label: pAcBAC3-POLY-pnRFP
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     714..733
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             763..780
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ataggtcggc tgggctcacc cgtagtttcc gagcggatgt accccgagga cggcgccctg
       61 aagagcgaga tcaagtaggg gctgaggctg aaggacggcg gccactacgc cgccgaggtc
      121 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc
      181 aagttggaca tcgtgtccca caacgaggac tacaccatcg tggaacagtg cgaacgcgcc
      241 gagggccgcc actccactgg cggcatggac gagctgtaca agggaggtac aggcgggagt
      301 ctggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      361 gtgcacatgg agggctccgt gaacggccac aagttcgaga tcgagggcga gggcgagggc
      421 cgcccctacg aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc
      481 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cattaagcac
      541 ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc
      601 gtggtgaact tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac
      661 ggcgtattca tctacaaggt gaagctgcgc ggcaccaact tcccccccga cggccccgta
      721 atgcagaaga agaccatggg ctgggaggct acgcgtgacc aacaccatca ccaccatcat
      781 taa
//
